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  • 1
    Online Resource
    Online Resource
    Wiley ; 2023
    In:  Medical and Veterinary Entomology Vol. 37, No. 1 ( 2023-03), p. 132-142
    In: Medical and Veterinary Entomology, Wiley, Vol. 37, No. 1 ( 2023-03), p. 132-142
    Abstract: As a widespread vector of disease with an expanding range, the mosquito Aedes albopictus Skuse (Diptera: Culicidae) is a high priority for research and management. A. albopictus has a complex life history with aquatic egg, larval and pupal stages, and a terrestrial adult stage. This requires targeted management strategies for each life stage, coordinated across time and space. Population genetics can aid in A. albopictus control by evaluating patterns of genetic diversity and dispersal. However, how life stage impacts population genetic characteristics is unknown. We examined whether patterns of A. albopictus genetic diversity and differentiation changed with life stage at a spatial scale relevant to management efforts. We first conducted a literature review of field‐caught A. albopictus population genetic papers and identified 101 peer‐reviewed publications, none of which compared results between life stages. Our study uniquely examines population genomic patterns of egg and adult A. albopictus at five sites in Wake County, North Carolina, USA, using 8425 single nucleotide polymorphisms. We found that the level of genetic diversity and connectivity between sites varied between adults and eggs. This warrants further study and is critical for research aimed at informing local management.
    Type of Medium: Online Resource
    ISSN: 0269-283X , 1365-2915
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2008073-6
    SSG: 22
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  • 2
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2019
    In:  Parasites & Vectors Vol. 12, No. 1 ( 2019-12)
    In: Parasites & Vectors, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2019-12)
    Abstract: The yellow fever mosquito, Aedes aegypti is a public health concern in the USA, especially in the wake of emergent diseases such as Zika and chikungunya. Aedes aegypti populations dwindled after the invasion of Aedes albopictus in the 1980s and many populations were extirpated. However, in some areas Ae. aegypti persisted in small populations and there are reports of recent resurgences of Ae. aegypti in Florida, Louisiana, Nevada and California. We assessed the population genetic structure of Ae. aegypti in Florida and Georgia, which has concomitant consequences related to mosquito dispersal, pesticide resistance and vectorial capacity. Methods We collected Ae. aegypti across Florida and in Georgia using ovitraps. We hatched the eggs and reared them to adults, and after sacrifice we extracted their DNA. We then probed each individual for variation in 6 microsatellite markers, which we used to address population genetic characteristics. Results We collected Ae. aegypti and genotyped seven Florida populations and one Georgia population using microsatellite markers. We found evidence of isolation by distance model of gene flow supported by driving distance among cities within Florida and two theoretic genetic clusters. Conclusions Significant genetic structure between some populations with substantial gene flow between geographically distant cities suggests regional genetic structuring of Ae. aegypti in Florida. This study provides information on the genetic exchange between populations of Ae. aegypti in the southeastern USA and suggests potential routes of spread of this species.
    Type of Medium: Online Resource
    ISSN: 1756-3305
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2409480-8
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  • 3
    Online Resource
    Online Resource
    Wiley ; 2018
    In:  Molecular Ecology Vol. 27, No. 18 ( 2018-09), p. 3641-3654
    In: Molecular Ecology, Wiley, Vol. 27, No. 18 ( 2018-09), p. 3641-3654
    Abstract: While few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. Aedes albopictus (the Asian tiger mosquito) was introduced into the naturalized range of Aedes aegypti (the yellow fever mosquito) in the United States in the mid‐1980s, resulting in the displacement of A. aegypti in much of the south‐eastern United States. The rapid displacement was likely due to the superior competitive ability of A. albopictus as larvae and asymmetric mating interference competition, in which male A. albopictus mate with and sterilize A. aegypti females, a process called “satyrization.” The goal of this study was to examine the genomic responses of a resident species to an invasive species in which the mechanism of character displacement is understood. We used double‐digest restriction enzyme DNA sequencing (dd RAD seq) to analyse outlier loci between selected and control lines of laboratory‐reared A. aegypti females from two populations (Tucson, AZ and Key West, Florida, USA ), and individual females classified as either “resisted” or “mated with” A. albopictus males via mating trials of wild‐derived females from four populations in Florida. We found significant outlier loci in comparing selected and control lines and between mated and nonmated A. aegypti females in the laboratory and wild‐derived populations, respectively. We found overlap in specific outlier loci between different source populations that support consistent genomic signatures of selection within A. aegypti . Our results point to regions of the A. aegypti genome and potential candidate genes that may be involved in mating behaviour, and specifically in avoiding interspecific mating choices.
    Type of Medium: Online Resource
    ISSN: 0962-1083 , 1365-294X
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
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  • 4
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-12-30)
    Abstract: The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher ( P 〈 0.05) on external body surfaces. At the genus level, Dysgonomonas , Ignatzschineria , Acinetobacter , Vagococcus , Myroides , and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2615211-3
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  • 5
    Online Resource
    Online Resource
    Oxford University Press (OUP) ; 2019
    In:  Journal of Medical Entomology Vol. 56, No. 3 ( 2019-04-16), p. 761-773
    In: Journal of Medical Entomology, Oxford University Press (OUP), Vol. 56, No. 3 ( 2019-04-16), p. 761-773
    Type of Medium: Online Resource
    ISSN: 0022-2585 , 1938-2928
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2019
    detail.hit.zdb_id: 2031006-7
    SSG: 12
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  • 6
    In: Pest Management Science, Wiley, Vol. 78, No. 7 ( 2022-07), p. 2931-2939
    Abstract: Aedes albopictus is a nuisance pest mosquito of public health importance commonly managed with adulticides and larvicides. We investigated whether adding Gravid Aedes Traps (GATs), Autocidal Gravid Ovitraps (AGOs) or In2Care traps would extend the effectiveness of chemical control methods in Wake County, North Carolina, USA, by combining barrier sprays and larval habitat management (LHM) with each trap type at suburban households. We compared these three treatment groups to untreated controls and to backyards treated only with barrier sprays and LHM. Once a week, for ten weeks, we collected adult mosquitoes at each house using lure‐baited surveillance traps and dissected a portion of Ae. albopictus females to determine parity. Results Barrier sprays and LHM alone or combined with any supplemental autocidal ovitrap significantly reduced female Ae. albopictus through Week 3 post‐treatment. GATs significantly extended chemical control effectiveness for the duration of the study. Compared to the untreated control, the AGO and GAT treatment groups had significant overall female Ae. albopictus reductions of 74% and 80.4%, respectively, with populations aging significantly slower at houses treated with AGOs. Conclusion This household‐level study, though limited in size, observed significant reductions in nuisance Ae. albopictus when combining AGOs and GATs with chemical controls for an eight‐week period. Delayed population aging in AGO‐treated yards suggests that traps also could mitigate disease transmission risk. Future studies should test these control methods at the neighborhood level to evaluate large‐scale effectiveness as well as assess the effect of autocidal ovitraps without chemical intervention. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 1526-498X , 1526-4998
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2003455-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2021
    In:  EcoHealth Vol. 18, No. 4 ( 2021-12), p. 475-486
    In: EcoHealth, Springer Science and Business Media LLC, Vol. 18, No. 4 ( 2021-12), p. 475-486
    Type of Medium: Online Resource
    ISSN: 1612-9202 , 1612-9210
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2146881-3
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  • 8
    Online Resource
    Online Resource
    American Mosquito Control Association ; 2020
    In:  Journal of the American Mosquito Control Association Vol. 36, No. 2 ( 2020-06-01), p. 74-80
    In: Journal of the American Mosquito Control Association, American Mosquito Control Association, Vol. 36, No. 2 ( 2020-06-01), p. 74-80
    Abstract: There is a clear need for improved vector surveillance approaches that are affordable, labor efficient, and safer than traditional methods. The BG-Counter (Biogents USA, Moorefield, WV) is a device for remotely monitoring mosquito activity in combination with the BG-Sentinel (Biogents USA), a widely used trap for the collection of host-seeking mosquitoes. The BG-Counter uses a wireless connection to provide real-time counts of mosquitoes captured by the BG-Sentinel, allowing users to remotely monitor mosquito populations. This study tested the effectiveness of the BG-Counter in 5 North Carolina counties. A total of 96 trap-days resulted in the collection of & gt;45,000 individual mosquitoes representing 35 species. Aedes albopictus was the most common species collected in all counties, except for New Hanover County where Culex nigripalpus was the most common. The mean daily accuracy ranged from 80.1% (New Hanover County) to 9.4% (Jackson County). There was a significant linear relationship between the actual number of mosquitoes collected and the device counts at all sites except Jackson County, the site with the lowest relative mosquito abundance compared with nontarget organisms. A linear regression of daily BG-Counter accuracy and the daily proportion of mosquitoes to the total number of arthropods collected revealed a significant positive linear relationship, supporting the premise that the BG-Counter is less effective when the relative abundance of mosquitoes is low. Mosquito surveillance programs using the BG-Counter should recognize its context-dependent accuracy and routinely evaluate the accuracy of the device based on local conditions.
    Type of Medium: Online Resource
    ISSN: 8756-971X , 1943-6270
    Language: English
    Publisher: American Mosquito Control Association
    Publication Date: 2020
    detail.hit.zdb_id: 2322636-5
    SSG: 12
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  • 9
    In: Journal of Medical Entomology, Oxford University Press (OUP), Vol. 56, No. 6 ( 2019-10-28), p. 1704-1714
    Abstract: Filth flies live in close proximity to humans and livestock and transmit pathogens. Current control relies on chemical insecticides, and flies can develop resistance to these insecticides. The public is also interested in natural and safer insecticides. Therefore, alternative pesticides compatible with the synanthropic nature of flies are needed. Four plant aliphatic methyl ketones were evaluated for control of adult house flies, Musca domestica L., blow flies, Cochliomyia macellaria (F.), and gray flesh flies, Sarcophaga bullata (Parker). In sealed petri dish assays, 2-heptanone, 2-octanone, 2-nonanone, and 2-undecanone exhibited fumigant activity against house flies with 24-h LC50s of 6.9, 7.5, 8.0, and 9.2 µg/cm3, respectively. Further research focused on undecanone (a U.S. EPA-registered biopesticide). When tested in larger enclosures at 1.7, 2.3, and 2.8 µg/cm3, undecanone provided 60.4, 82.2, and 94.4% house fly mortality; 56.9, 75.6, and 92.5% flesh fly mortality; and 62.1, 84.5, and 97.9% blow fly mortality, respectively, after a 2-h exposure. In a two-choice behavioral assay with 194.6 µg/cm2 of the test compound on the treatment versus an untreated surface of the same area, the overall mean repellencies for blow flies were 84.7% for undecanone versus 87.6% for N,N-diethyl-meta-toluamide (DEET). For house flies, mean repellencies were 80.7% for undecanone and 84.9% for DEET. The house fly topical LD50 for undecanone was 58.1 µg per fly. Undecanone was far less expensive for filth fly control than the gold standard for insect fumigation, methyl bromide.
    Type of Medium: Online Resource
    ISSN: 0022-2585 , 1938-2928
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2019
    detail.hit.zdb_id: 2031006-7
    SSG: 12
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  • 10
    In: Environmental Health Insights, SAGE Publications, Vol. 14 ( 2020-01), p. 117863022095280-
    Abstract: Container Aedes mosquitoes are responsible for the transmission of anthroponotic and zoonotic viruses to people. The surveillance and control of these mosquitoes is an important part of public health protection and prevention of mosquito-borne disease. In this study, we surveyed 327 sites over 2 weeks in late June and early July in 2017 in North Carolina, USA for the presence and abundance of Aedes spp. eggs in an effort to better target potential Ae. aegypti collections. We examined the ability of 2 types of landscape data, Light Detection And Ranging (LIDAR) and National Land Cover Database (NLCD) to explain the presence and abundance of eggs using principal component analysis to deal with collinearity, followed by generalized linear regression. We explained variation of both egg presence and abundance for Aedes albopictus (Skuse) and Aedes triseriatus (Say) using both NLCD and LIDAR data. However, the ability to make robust predictions was limited by variation in the data. Increased sampling time and better landscape data would likely improve the predictive ability of our models, as would a better understanding of oviposition behavior.
    Type of Medium: Online Resource
    ISSN: 1178-6302 , 1178-6302
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2452946-1
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