In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 7 ( 2023-7-6), p. e3002182-
Abstract:
The viral hemagglutinins of conventional influenza A viruses (IAVs) bind to sialylated glycans on host cell surfaces for attachment and subsequent infection. In contrast, hemagglutinins of bat-derived IAVs target major histocompatibility complex class II (MHC-II) for cell entry. MHC-II proteins from various vertebrate species can facilitate infection with the bat IAV H18N11. Yet, it has been difficult to biochemically determine the H18:MHC-II binding. Here, we followed a different approach and generated MHC-II chimeras from the human leukocyte antigen DR (HLA-DR), which supports H18-mediated entry, and the nonclassical MHC-II molecule HLA-DM, which does not. In this context, viral entry was supported only by a chimera containing the HLA-DR α1, α2, and β1 domains. Subsequent modeling of the H18:HLA-DR interaction identified the α2 domain as central for this interaction. Further mutational analyses revealed highly conserved amino acids within loop 4 (N149) and β-sheet 6 (V190) of the α2 domain as critical for virus entry. This suggests that conserved residues in the α1, α2, and β1 domains of MHC-II mediate H18-binding and virus propagation. The conservation of MHC-II amino acids, which are critical for H18N11 binding, may explain the broad species specificity of this virus.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002182
DOI:
10.1371/journal.pbio.3002182.g001
DOI:
10.1371/journal.pbio.3002182.g002
DOI:
10.1371/journal.pbio.3002182.g003
DOI:
10.1371/journal.pbio.3002182.g004
DOI:
10.1371/journal.pbio.3002182.s001
DOI:
10.1371/journal.pbio.3002182.s002
DOI:
10.1371/journal.pbio.3002182.s003
DOI:
10.1371/journal.pbio.3002182.s004
DOI:
10.1371/journal.pbio.3002182.s005
DOI:
10.1371/journal.pbio.3002182.s006
DOI:
10.1371/journal.pbio.3002182.s007
DOI:
10.1371/journal.pbio.3002182.s008
DOI:
10.1371/journal.pbio.3002182.s009
DOI:
10.1371/journal.pbio.3002182.s010
DOI:
10.1371/journal.pbio.3002182.s011
DOI:
10.1371/journal.pbio.3002182.s012
DOI:
10.1371/journal.pbio.3002182.s013
DOI:
10.1371/journal.pbio.3002182.s014
DOI:
10.1371/journal.pbio.3002182.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
Bookmarklink