In:
Journal of Veterinary Medicine Series A, Wiley, Vol. 40, No. 1-10 ( 1993-02-12), p. 283-291
Abstract:
The in vitro cation‐induced aggregation properties of cat Tamm‐Horsfall protein (cTHP), a urinary glycoprotein, were examined and related to the potential role of cTHP in feline urolithiasis. The aggregation assay involved adding either CaCl 2 , MgCl 2 , or NaCl to solutions containing purified cTHP, and then separating the aggregated cTHP by centrifugation. The concentration of cTHP remaining in the supernatant was quantified using a previously developed enzyme‐linked immunosorbent assay (ELISA). The effect that buffer pH, cTHP concentration, and urea concentration had on cTHP aggregation also were examined. Of the three salts, CaCl 2 consistently was most efficient at precipitating cTHP, while MgCl 2 was slightly less efficient. At least ten times more NaCl than CaCl 2 or MgCl 2 was required for comparable cTHP aggregation. As the pH decreased, increasing concentrations of the salts were required to aggregate cTHP. Increased amounts of CaCl 2 and MgCl 2 also were required to aggregate cTHP when the urea concentration was increased. As cTHP concentration increased within the physiological range, lower concentrations of CaCl 2 and MgCl 2 were required to precipitate 50% of the cTHP. Several aspects of the in vitro aggregation properties of cTHP correlate closely with previously identified risk factors for feline urolithiasis, strengthening the theory that cTHP aggregation may be important in this disease.
Type of Medium:
Online Resource
ISSN:
0931-184X
DOI:
10.1111/tbed.1993.40.issue-1-10
DOI:
10.1111/j.1439-0442.1993.tb00629.x
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
2414822-2
SSG:
22
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