In:
FEBS Letters, Wiley, Vol. 450, No. 1-2 ( 1999-04-30), p. 44-48
Abstract:
The steady state single electron reduction of polynitroaromatics by ferredoxin‐NADP + oxidoreductase (EC 1.18.1.2) from cyanobacterium Anabaena PCC 7119 has been studied and quantitative structure activity relationships are described. The solubility of the polynitroaromatics as well as their reactivity towards ferredoxin‐NADP + oxidoreductase are markedly higher than those for previously studied mononitroaromatics and this enabled the independent measurement of the kinetic parameters k cat and K m . Interestingly, the natural logarithm of the bimolecular rate constant, k cat / K m , and also the natural logarithm of k cat correlate with the calculated energy of the lowest unoccupied molecular orbital of the polynitroaromatic substrates. The minimal kinetic model in line with these quantitative structure activity relationships is a ping‐pong mechanism which includes substrate binding equilibria in the second half reaction.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(99)00464-0
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
212746-5
detail.hit.zdb_id:
1460391-3
SSG:
12
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