In:
Plant Disease, Scientific Societies, Vol. 94, No. 7 ( 2010-07), p. 920-920
Abstract:
In 2008, in the framework of surveying for pospiviroids, nine symptomless clones of Celosia plumosa (Voss) Burv. (Amaranthaceae) from a Dutch breeding company were tested by reverse transcription (RT)-PCR with primer sets Pospi1-RE/FW and Vid-RE/FW (4). In four samples, amplicons of 227 nt were obtained with primers Pospi1-RE/FW. Sequencing of the amplicons showed identities of more than 99% to the partial sequence of Iresine viroid 1 (IrVd-1) from Alternanthera sessilis, NCBI GenBank Accession No DQ846886 (2). Subsequently, a set of primers was designed to amplify the complete viroid genome, i.e., IrVd-FW1 5′-GCG GAA GAA ACA GGA GCT CGW CT-3′ and IrVd-RE1 5′-CGC GWG GAG TTC TCC GGT CTT TA-3′ – identical to nt 168 to 190 and 145 to 167 of the complete IrVd-1 sequences in the NCBI GenBank (Nos. DQ094293, DQ094294, NC_003613, and X95734). One isolate from C. plumosa was amplified with this primer pair and amplicons were cloned into the pGEM-T Easy Vector System II. Sequencing of one individual cDNA clone (GenBank Accession No. GU911350) revealed a genome size of 370 nt and 98.1% sequence identity to the IrVd–1 isolate from Vinca major, GenBank Accession No. DQ094293 (1). Hence, the viroid was identified as IrVd-1. The isolate from C. plumosa was also mechanically inoculated to 10 healthy plants of C. plumosa, chrysanthemum (Chrysanthemum × morifolium) cv. White Delianne, potato (Solanum tuberosum) cv. Nicola, and tomato (Solanum lycopersicum) cv. Moneymaker. No symptoms were observed over a 6-week period, and RT-PCR with primers Pospi1-RE/FW on bulked samples of five plants per species only identified IrVd-1 in both samples of C. plumosa. For tomato, these results confirm those of Spieker (3). Therefore, in contrast to the other pospiviroids, it seems unlikely that IrVd-1 poses a threat to potato and tomato. References: (1) X. Nie et al. Can. J. Plant Pathol. 27:592, 2005. (2) R. P. Singh et al. Plant Dis. 90:1457, 2006. (3) R. L. Spieker. J. Gen. Virol. 77:2631, 1996. (4) J. Th. J. Verhoeven et al. Eur. J. Plant Pathol. 110:823, 2004.
Type of Medium:
Online Resource
ISSN:
0191-2917
,
1943-7692
DOI:
10.1094/PDIS-94-7-0920C
Language:
English
Publisher:
Scientific Societies
Publication Date:
2010
detail.hit.zdb_id:
2042679-3
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