In:
Otolaryngology–Head and Neck Surgery, Wiley, Vol. 121, No. 1 ( 1999-07), p. 7-12
Abstract:
This study addresses the interaction of bacterial antigens, specifically peptidoglycan‐polysaccharide (PG‐PS) and lipopolysaccharide (LPS), in the induction and reactivation of mucoid middle ear effusions. METHODS Twenty‐seven rats underwent eustachian tube obstruction before inoculation of the middle ear bulla with PG‐PS. Three weeks later, after resolution of all middle ear effusions, 6 rats were randomly selected and euthanized as the first control group (control I). The remaining 21 animals were randomly assigned to 3 groups that received intravenous injections of Krebs Ringer (control II), PG‐PS, and LPS, respectively. These rats were euthanized 2 days after intravenous injection. Middle ear mucin production and histologic changes were measured in all animals. RESULTS The mean concentrations of mucin were 0.94 ± 0.52 mg/mL, 0.41 ± 0.87 mg/mL, 16.33 ± 3.67 mg/mL, and 1.15 ± 0.41 mg/mL in the control I, control II, PG‐PS, and LPS groups, respectively. Thus the mean concentration of mucin in the middle ear lavage samples was significantly greater in rats that were injected intravenously with PG‐PS than in rats in other groups ( P 〈 0.05). Histologic analyses demonstrated a greater degree of goblet cell hyperplasia in the PG‐PS group than in other groups. CONCLUSIONS This is the first animal model of recurring otitis media with effusion in which a systemic injection of PG‐PS was used to reactivate a middle ear effusion in rats previously primed with a transtympanic injection of PG‐PS. This study suggests that after otitis media with effusion has resolved, it may be reactivated by the presence of bacterial antigens and/or cytokines in the systemic circulation.
Type of Medium:
Online Resource
ISSN:
0194-5998
,
1097-6817
DOI:
10.1016/S0194-5998(99)70114-2
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
2008453-5
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