In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 20, No. 2 ( 2024-2-26), p. e1011942-
Abstract:
Highly pathogenic avian influenza viruses (HPAIVs) cause severe hemorrhagic disease in terrestrial poultry and are a threat to the poultry industry, wild life, and human health. HPAIVs arise from low pathogenic avian influenza viruses (LPAIVs), which circulate in wild aquatic birds. HPAIV emergence is thought to occur in poultry and not wild aquatic birds, but the reason for this species-restriction is not known. We hypothesized that, due to species-specific tropism and replication, intrahost HPAIV selection is favored in poultry and disfavored in wild aquatic birds. We tested this hypothesis by co-inoculating chickens, representative of poultry, and ducks, representative of wild aquatic birds, with a mixture of H7N7 HPAIV and LPAIV, mimicking HPAIV emergence in an experimental setting. Virus selection was monitored in swabs and tissues by RT-qPCR and immunostaining of differential N-terminal epitope tags that were added to the hemagglutinin protein. HPAIV was selected in four of six co-inoculated chickens, whereas LPAIV remained the major population in co-inoculated ducks on the long-term, despite detection of infectious HPAIV in tissues at early time points. Collectively, our data support the hypothesis that HPAIVs are more likely to be selected at the intrahost level in poultry than in wild aquatic birds and point towards species-specific differences in HPAIV and LPAIV tropism and replication levels as possible explanations.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011942
DOI:
10.1371/journal.ppat.1011942.g001
DOI:
10.1371/journal.ppat.1011942.g002
DOI:
10.1371/journal.ppat.1011942.g003
DOI:
10.1371/journal.ppat.1011942.g004
DOI:
10.1371/journal.ppat.1011942.g005
DOI:
10.1371/journal.ppat.1011942.g006
DOI:
10.1371/journal.ppat.1011942.g007
DOI:
10.1371/journal.ppat.1011942.s001
DOI:
10.1371/journal.ppat.1011942.s002
DOI:
10.1371/journal.ppat.1011942.s003
DOI:
10.1371/journal.ppat.1011942.s004
DOI:
10.1371/journal.ppat.1011942.s005
DOI:
10.1371/journal.ppat.1011942.s006
DOI:
10.1371/journal.ppat.1011942.s007
DOI:
10.1371/journal.ppat.1011942.s008
DOI:
10.1371/journal.ppat.1011942.s009
DOI:
10.1371/journal.ppat.1011942.s010
DOI:
10.1371/journal.ppat.1011942.s011
DOI:
10.1371/journal.ppat.1011942.s012
DOI:
10.1371/journal.ppat.1011942.s013
DOI:
10.1371/journal.ppat.1011942.s014
DOI:
10.1371/journal.ppat.1011942.s015
DOI:
10.1371/journal.ppat.1011942.s016
DOI:
10.1371/journal.ppat.1011942.r001
DOI:
10.1371/journal.ppat.1011942.r002
DOI:
10.1371/journal.ppat.1011942.r003
DOI:
10.1371/journal.ppat.1011942.r004
DOI:
10.1371/journal.ppat.1011942.r005
DOI:
10.1371/journal.ppat.1011942.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2024
detail.hit.zdb_id:
2205412-1
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