In:
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, SAGE Publications, Vol. 54, No. 5 ( 2017-09), p. 539-547
Abstract:
Inflammation and hypercholesterolaemia contribute to atherosclerotic changes which can start in childhood. Children with hyperlipidaemias are at high risk for early coronary atherosclerosis. This study evaluates the relationship between lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ), carotid intima-media thickness (CIMT) and flow-mediated dilatation in hypercholesterolaemic dyslipidaemic children. Methods We performed a case–control study consisting of 43 cases, aged 2 to 17 years, and 24 age-matched controls. Fasting blood samples were obtained from both groups for the measurement of a lipid profile (total cholesterol, LDL-C, HDL-C and triglycerides) and Lp-PLA 2 in mass units. The latter was determined with a turbidimetric immunoassay method (PlacTest, DiaDexus Inc.) applied to an automated analyser. CIMT and flow-mediated dilatation measurements were undertaken by a paediatric cardiologist, using high-resolution B-mode ultrasonography. Results Total cholesterol, LDL-C and Lp-PLA 2 concentrations were significantly higher in the cases than in the controls ( p 〈 0.001 for all three parameters). While CIMT values were also significantly higher in the patients compared to the controls ( P = 0.001), flow-mediated dilatation values were significantly lower ( P = 0.001). We found positive correlations between Lp-PLA 2 and total cholesterol ( r = 0.41, P = 0.001), Lp-PLA 2 and LDL-C ( r = 0.36, P = 0.004), Lp-PLA 2 and CIMT ( r = 0.44, P = 0.019) and LDL-C and CIMT ( r = 0.41, P = 0.032); there were negative correlations between Lp-PLA 2 and flow-mediated dilatation ( r = −0.15, P = 0.045), total cholesterol and flow-mediated dilatation ( r = −0.45, P = 0.017), LDL-C and flow-mediated dilatation ( r = −0.51, P = 0.006) and CIMT and flow-mediated dilatation ( r = −0.45, P = 0.016). Conclusion Lp-PLA 2 concentrations are significantly elevated in hypercholesterolaemic dyslipidaemic children. Given the association of Lp-PLA 2 with markers of atherosclerosis (total cholesterol, LDL-C, CIMT and flow-mediated dilatation), the finding of increased concentrations of Lp-PLA 2 could be used to identify early atherosclerotic changes in hypercholesterolaemic dyslipidaemic children and may inform their clinical management.
Type of Medium:
Online Resource
ISSN:
0004-5632
,
1758-1001
DOI:
10.1177/0004563216671338
Language:
English
Publisher:
SAGE Publications
Publication Date:
2017
detail.hit.zdb_id:
2041298-8
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