In:
European Journal of Clinical Investigation, Wiley, Vol. 20, No. s1 ( 1990-10), p. 540-545
Abstract:
Abstract. The pre‐S 2 protein of the hepatitis B virus envelope evokes anti‐pre‐S 2 antibodies and enhances the anti‐HBs response after vaccination in mice. In order to evaluate the immunogenicity of the pre‐S 2 Ag in man, 102 HBsAg, anti‐HBs, anti‐HBc‐negative haemodialysis patients were vaccinated at random according to one of four vaccination schedules: 5 μg plasma vaccine Pasteur (containing 1 % pre‐S 2 ) or 20 μg plasma vaccine MSD (no pre‐S 2 ) at 0, 1, 2, 4, 6 and 12 months; or 10 μg Pasteur or 40 μg MSD at 0, 1,2 and 6 months. Anti‐HBs levels were measured by RIA and expressed in IU 1 ‐1 ; anti‐pre‐S 2 response was evaluated by both EIA and Western blot analysis. Eighty‐four per cent (95% confidence interval: 75–93) of the patients exhibited an anti‐HBs response of 2 IU l ‐1 or more and 71% (95% confidence interval: 61–81) reached an anti‐HBs level of at least 10 IU l ‐1 within 13 months of the start of vaccination. Anti‐HBs response correlated with age (the response rate decreased with increasing age) but not with either the type of vaccine or dosage. An anti‐pre‐S 2 response was observed in 16% (EIA) and 46% (Western blot) of the patients immunized with the Pasteur vaccine and none (EIA, Western blot) of the MSD group. The level of anti‐pre‐S 2 antibodies correlated with high anti‐HBs titres; an anti‐pre‐S 2 response occurred in only one anti‐HBs‐negative patient. The addition of 1% pre‐S 2 to the HBsAg plasma hepatitis B vaccine had no significant effect on the anti‐HBs response in haemodialysis patients and did not markedly broaden protective immunity. Further studies of recombinant vaccines with a higher pre‐S 2 content are needed to document the possibility of enhanced immunogenicity of pre‐S 2 Ag‐containing hepatitis B vaccines in man.
Type of Medium:
Online Resource
ISSN:
0014-2972
,
1365-2362
DOI:
10.1111/eci.1990.20.issue-s1
DOI:
10.1111/j.1365-2362.1990.tb01924.x
Language:
English
Publisher:
Wiley
Publication Date:
1990
detail.hit.zdb_id:
2004971-7
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