In:
Journal of Cell Science, The Company of Biologists
Kurzfassung:
Chromatin function is involved in many cellular processes, its visualization or modification being essential in many developmental or cellular studies. Here, we present the characterization of chromatibody, a chromatin-binding single-domain, and explore its use in living cells. This non-intercalating tool specifically binds the heterodimer of H2A-H2B histones and displays a versatile reactivity, specifically labeling chromatin from yeast to mammals. We show that this genetically-encoded probe, when fused to fluorescent proteins, allows non–invasive real-time chromatin imaging. Chromatibody is a dynamic chromatin probe that can be modulated. Finally, chromatibody is an efficient tool to target an enzymatic activity to the nucleosome, such as the DNA damage-dependent H2A ubiquitination, modifying this epigenetic mark at the scale of the genome and resulting in DNA damage signaling and repair defects. Altogether, these results identify chromatibody as a universal non-invasive tool for either in vivo chromatin imaging or to manipulate the chromatin landscape.
Materialart:
Online-Ressource
ISSN:
1477-9137
,
0021-9533
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
2016
ZDB Id:
219171-4
ZDB Id:
1483099-1
SSG:
12
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