In:
Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 49, No. 11 ( 2003-11-01), p. 723-726
Abstract:
Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase.Key words: Metarhizium anisopliae, chitinases, chit genes, recombinant protein, enthomopathogenic fungi.
Type of Medium:
Online Resource
ISSN:
0008-4166
,
1480-3275
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2003
detail.hit.zdb_id:
280534-0
detail.hit.zdb_id:
1481972-7
SSG:
12
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