In:
The Journal of Immunology, The American Association of Immunologists, Vol. 194, No. 1_Supplement ( 2015-05-01), p. 58.13-58.13
Abstract:
The balance between Th17 and Tregs influences the pathogenesis of autoimmune diseases. The active form of vitamin D, 1α,25-dihydroxyvitamin D3, was shown to exert anti-inflammatory effect. The aim of this study is to investigate the effect of vitamin D on the differentiation of naïve and total CD4+ T cells into pathogenic Th17 subset and regulatory T cells ex-vivo in healthy controls, and to characterize their phenotype and its modulation by vitamin D. Purified naïve and total CD4+ T cells were activated in Th17 and Treg polarizing conditions in the presence or absence of 1,25(OH)2D3 for 6 days. Cells were processed for multiparameter staining and flow cytometry, and cell culture supernatants were reserved for cytokine quantitation by cytometric bead array for IL-17A, IL-10, IL-6, TNF-α, IL-2, IFN-γ, and IL-4. In Th17 cell cultures, overall expression of CCR6 and the percentage of CCR6+IL-17A+CD4+ T cells is decreased with Vitamin D. Interestingly, IL-23R and IL-23R+IL-17A+ cells, as well as IL-17+IFN-γ+ cells associated with pathogenic Th17 cells are reduced, while Foxp3+ cells are increased. In the presence of vitamin D, Treg polarized CD4+ T cells upregulated CD25 and CTLA4 expression but not Foxp3. Foxp3 expression was reduced in both CD25hi and CD25lo cells. Cytokine analysis by CBA and Treg suppression assays are underway. Results obtained thus far point to an effect for Vitamin D in reducing pathogenic Th17 cells, and modulating the induction of regulatory T cells.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.194.Supp.58.13
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2015
detail.hit.zdb_id:
1475085-5
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