In:
Angewandte Chemie, Wiley, Vol. 134, No. 22 ( 2022-05-23)
Abstract:
In the FeGP cofactor of [Fe]‐hydrogenase, low‐spin Fe II is in complex with two CO ligands and a pyridinol derivative; the latter ligates the iron with a 6‐acylmethyl substituent and the pyridinol nitrogen. A guanylylpyridinol derivative, 6‐carboxymethyl‐3,5‐dimethyl‐4‐guanylyl‐2‐pyridinol ( 3 ), is produced by the decomposition of the FeGP cofactor under irradiation with UV‐A/blue light and is also postulated to be a precursor of FeGP cofactor biosynthesis. HcgC and HcgB catalyze consecutive biosynthesis steps leading to 3 . Here, we report an in vitro biosynthesis assay of the FeGP cofactor using the cell extract of the Δ hcgB Δ hcgC strain of Methanococcus maripaludis , which does not biosynthesize 3 . We chemically synthesized pyridinol precursors 1 and 2 , and detected the production of the FeGP cofactor from 1 , 2 and 3 . These results indicated that 1 , 2 and 3 are the precursors of the FeGP cofactor, and the carboxy group of 3 is converted to the acyl ligand.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v134.22
DOI:
10.1002/ange.202200994
Language:
English
Publisher:
Wiley
Publication Date:
2022
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