In:
eLife, eLife Sciences Publications, Ltd, Vol. 5 ( 2016-07-07)
Abstract:
Neurons connect with each other to form complex circuits that underlie mental activities. Mapping these connections to obtain a so-called wiring diagram is an essential step in learning how the brain works. The only way to do this precisely enough is by using electron microscopy. However, this technique is so time-consuming that thousands of hours of work are typically required to image even the smallest of tissue samples. Electron microscopes fire beams of electrons at tissue samples, and detect the scattering of the electrons. Stains are used to make specific neurons less permeable to electrons, or more “electron dense”. Labeled cells scatter more electrons, which increases the contrast of the images. In an approach called serial-section electron microscopy, a tissue sample is first cut into extremely thin sections. These are imaged individually, and the images are then pieced together to reconstruct the sample. Joesch et al. have now developed a new procedure – named ARTEMIS – that uses a combination of multiple techniques to speed up the mapping of neurons and their connections. ARTEMIS makes use of genetic engineering, serial-scanning electron microscopy, an enhanced chemical staining procedure and a new image processing approach. First, gene technology is used to selectively stain specific types of neurons in mice and flies. Then, a tissue sample is collected and treated with a chemical that enhances the electron density of the stained neurons, without disrupting the tissue’s structure. Next, a labeled target neuron is imaged at relatively low resolution to reveal its overall structure. Small areas of that neuron are then re-imaged at higher resolution to map the connections between neurons. Lastly, an algorithm pieces together the individual images to produce a reconstruction of the cell. This pipeline of steps reduces the time required to map the shapes and connectivity of neurons with electron microscopy by some two orders of magnitude. This should enable neuroscientists to obtain more rapid insights into the roles of specific neural circuits in the brains of healthy animals, and to identify cases where this wiring goes awry and leads to disease.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.15015.001
DOI:
10.7554/eLife.15015.002
DOI:
10.7554/eLife.15015.003
DOI:
10.7554/eLife.15015.004
DOI:
10.7554/eLife.15015.005
DOI:
10.7554/eLife.15015.006
DOI:
10.7554/eLife.15015.007
DOI:
10.7554/eLife.15015.008
DOI:
10.7554/eLife.15015.009
DOI:
10.7554/eLife.15015.010
DOI:
10.7554/eLife.15015.011
DOI:
10.7554/eLife.15015.012
DOI:
10.7554/eLife.15015.013
DOI:
10.7554/eLife.15015.014
DOI:
10.7554/eLife.15015.015
DOI:
10.7554/eLife.15015.018
DOI:
10.7554/eLife.15015.019
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2016
detail.hit.zdb_id:
2687154-3
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