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Moist inhalation in children : Importance of the aerodynamic diameter

Scherließ, Regina ; Neustock, Anna ; Struzek, Anna-Maria ; [et al.]

Springer Science and Business Media LLC ; 2023

In: Zeitschrift für Pneumologie

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In: Zeitschrift für Pneumologie, Springer Science and Business Media LLC

Abstract: Diseases of the respiratory tract are among the most common diseases in childhood. For inhalation treatment to be successful, nebulized droplets within a certain size range are required, which must be particularly small ( 〈  3 µm) for children. Aerodynamic characterization of droplet size, here using the MicroDrop® Calimero 2 nebulizer (MPV Medical, Parsdorf, Germany) as an example, is used to assess the dispersion capacity of a particular nebulizer-product combination. Objective The characterization of a nebulizer according to pharmacopeial methods is demonstrated exemplarily for one drug product and different nebulizer settings in order to investigate the influence on the deposition profile and the mass-related median aerodynamic diameter of nebulized droplets. Material and methods Aerodynamic characterization of SalbuHEXAL® 5 mg/ml nebulized with the MicroDrop® Calimero 2 nebulizer and MicroDrop® Calimero 2 compressor, in a cooled next generation pharmaceutical impactor (NGI). Active substance analysis was carried out by HPLC/Copley inhaler testing data analysis software. Results and conclusion The MicroDrop® Calimero 2 successfully nebulizes drug solutions in an aerodynamic droplet size well suited for inhalation into the lungs. By changing the speed controller setting to minimum/maximum air supply the aerosol output is varied but the resulting droplet size is not significantly affected.

Type of Medium: Online Resource

ISSN: 2731-7404 , 2731-7412

URL: Article

DOI: 10.1007/s10405-023-00524-x

Language: German

Publisher: Springer Science and Business Media LLC

Publication Date: 2023

detail.hit.zdb_id: 3120281-0

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Induction of protective immunity against H1N1 influenza A(H1N1)pdm09 with spray-dried and electron-beam sterilised vaccines in non-human primates

Scherließ, Regina ; Ajmera, Ankur ; Dennis, Mike ; [et al.]

Elsevier BV ; 2014

In: Vaccine Vol. 32, No. 19 ( 2014-04), p. 2231-2240

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In: Vaccine, Elsevier BV, Vol. 32, No. 19 ( 2014-04), p. 2231-2240

Type of Medium: Online Resource

ISSN: 0264-410X

URL: Article

DOI: 10.1016/j.vaccine.2014.01.077

Language: English

Publisher: Elsevier BV

Publication Date: 2014

detail.hit.zdb_id: 1468474-3

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Quantitative Analysis of Glassy State Relaxation and Ostwald Ripening during Annealing Using Freeze-Drying Microscopy

Kharatyan, Tigran ; Gopireddy, Srikanth R. ; Ogawa, Toru ; [et al.]

MDPI AG ; 2022

In: Pharmaceutics Vol. 14, No. 6 ( 2022-05-30), p. 1176-

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In: Pharmaceutics, MDPI AG, Vol. 14, No. 6 ( 2022-05-30), p. 1176-

Abstract: Supercooling during the freezing of pharmaceutical solutions often leads to suboptimal freeze-drying results, such as long primary drying times or a collapse in the cake structure. Thermal treatment of the frozen solution, known as annealing, can improve those issues by influencing properties such as the pore size and collapse temperature of the lyophilisate. In this study we aimed to show that annealing causes a rearrangement of water molecules between ice crystals, as well as between the freeze-concentrated amorphous matrix and the crystalline ice phase in a frozen binary aqueous solution. Ice crystal sizes, as well as volume fractions of the crystalline and amorphous phases of 10% (w/w) sucrose and trehalose solutions, were quantified after annealing using freeze-drying microscopy and image labelling. Depending on the annealing time and temperature, the amorphous phase was shown to decrease its volume due to the crystallisation of vitreous water (i.e., glassy state relaxation) while the crystalline phase was undergoing coarsening (i.e., Ostwald ripening). These results allow, for the first time, a quantitative comparison of the two phenomena. It was demonstrated that glassy state relaxation and Ostwald ripening, although occurring simultaneously, are distinct processes that follow different kinetics.

Type of Medium: Online Resource

ISSN: 1999-4923

URL: Article

DOI: 10.3390/pharmaceutics14061176

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2527217-2

SSG: 15,3

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Spatial separation of the processing and MHC class I loading compartments for cross-presentation of the tumor-associated antigen HER2/ neu by human dendritic cells

Baleeiro, Renato B ; Rietscher, René ; Diedrich, Andrea ; [et al.]

Informa UK Limited ; 2015

In: OncoImmunology Vol. 4, No. 11 ( 2015-11-02), p. e1047585-

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In: OncoImmunology, Informa UK Limited, Vol. 4, No. 11 ( 2015-11-02), p. e1047585-

Type of Medium: Online Resource

ISSN: 2162-402X

URL: Article

DOI: 10.1080/2162402X.2015.1047585

Language: English

Publisher: Informa UK Limited

Publication Date: 2015

detail.hit.zdb_id: 2645309-5

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Utilizing ICG Spectroscopical Properties for Real-Time Nanoparticle Release Quantification In vitro and In vivo in Imaging Setups

Peñate-Medina, Tuula ; Kraas, Eike ; Luo, Kunliang ; [et al.]

Bentham Science Publishers Ltd. ; 2020

In: Current Pharmaceutical Design Vol. 26, No. 31 ( 2020-09-17), p. 3828-3833

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In: Current Pharmaceutical Design, Bentham Science Publishers Ltd., Vol. 26, No. 31 ( 2020-09-17), p. 3828-3833

Abstract: Nanoparticle imaging and tracking the release of the loaded material from the nanoparticle system have attracted significant attention in recent years. If the release of the loaded molecules could be monitored reliably in vivo, it would speed up the development of drug delivery systems remarkably. Methods: Here, we test a system that uses indocyanine green (ICG) as a fluorescent agent for studying release kinetics in vitro and in vivo from the lipid iron nanoparticle delivery system. The ICG spectral properties like its concentration dependence, sensitivity and the fluctuation of the absorption and emission wavelengths can be utilized for gathering information about the change of the ICG surrounding. Results: We have found that the absorption, fluorescence, and photoacoustic spectra of ICG in lipid iron nanoparticles differ from the spectra of ICG in pure water and plasma. We followed the ICG containing liposomal nanoparticle uptake into squamous carcinoma cells (SCC) by fluorescence microscopy and the in vivo uptake into SCC tumors in an orthotopic xenograft nude mouse model under a surgical microscope. Conclusion: Absorption and emission properties of ICG in the different solvent environment, like in plasma and human serum albumin, differ from those in aqu eous solution. Photoacoustic spectral imaging confirmed a peak shift towards longer wavelengths and an intensity increase of ICG when bound to the lipids. The SCC cells showed that the ICG containing liposomes bind to the cell surface but are not internalized in the SCC-9 cells after 60 minutes of incubation. We also showed here that ICG containing liposomal nanoparticles can be traced under a surgical camera in vivo in orthotopic SCC xenografts in mice.

Type of Medium: Online Resource

ISSN: 1381-6128

URL: Article

DOI: 10.2174/1381612826666200318170849

Language: English

Publisher: Bentham Science Publishers Ltd.

Publication Date: 2020

SSG: 15,3

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Establishment of a Rodent Glioblastoma Partial Resection Model for Chemotherapy by Local Drug Carriers—Sharing Experience

Kubelt, Carolin ; Hellmold, Dana ; Peschke, Eva ; [et al.]

MDPI AG ; 2023

In: Biomedicines Vol. 11, No. 6 ( 2023-05-24), p. 1518-

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In: Biomedicines, MDPI AG, Vol. 11, No. 6 ( 2023-05-24), p. 1518-

Abstract: Local drug delivery systems (LDDS) represent a promising therapy strategy concerning the most common and malignant primary brain tumor glioblastoma (GBM). Nevertheless, to date, only a few systems have been clinically applied, and their success is very limited. Still, numerous new LDDS approaches are currently being developed. Here, (partial resection) GBM animal models play a key role, as such models are needed to evaluate the therapy prior to any human application. However, such models are complex to establish, and only a few reports detail the process. Here, we report our results of establishing a partial resection glioma model in rats suitable for evaluating LDDS. C6-bearing Wistar rats and U87MG-spheroids- and patient-derived glioma stem-like cells-bearing athymic rats underwent tumor resection followed by the implantation of an exemplary LDDS. Inoculation, tumor growth, residual tumor tissue, and GBM recurrence were reliably imaged using high-resolution Magnetic Resonance Imaging. The release from an exemplary LDDS was verified in vitro and in vivo using Fluorescence Molecular Tomography. The presented GBM partial resection model appears to be well suited to determine the efficiency of LDDS. By sharing our expertise, we intend to provide a powerful tool for the future testing of these very promising systems, paving their way into clinical application.

Type of Medium: Online Resource

ISSN: 2227-9059

URL: Article

DOI: 10.3390/biomedicines11061518

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2720867-9

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Autologous Co-culture of Primary Human Alveolar Macrophages and Epithelial Cells for Investigating Aerosol Medicines. Part I: Model Characterisation

Hittinger, Marius ; Janke, Julia ; Huwer, Hanno ; [et al.]

SAGE Publications ; 2016

In: Alternatives to Laboratory Animals Vol. 44, No. 4 ( 2016-09), p. 337-347

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In: Alternatives to Laboratory Animals, SAGE Publications, Vol. 44, No. 4 ( 2016-09), p. 337-347

Abstract: The development of new formulations for pulmonary drug delivery is a challenge on its own. New in vitro models which address the lung are aimed at predicting and optimising the quality, efficacy and safety of inhaled drugs, to facilitate the more rapid translation of such products into the clinic. Reducing the complexity of the in vivo situation requires that such models reproducibly reflect essential physiological factors in vitro. The choice of cell types, culture conditions and the experimental set-up, can affect the outcome and the relevance of a study. In the alveolar space of the lung, epithelial cells and alveolar macrophages are the most important cell types, forming an efficient cellular barrier to aerosols. Our aim was to mimic this barrier with primary human alveolar cells. Cell densities of alveolar macrophages and epithelial cells, isolated from the same human donor, were optimised, with a focus on barrier properties. The combination of 300,000 epithelial cells/cm 2 together with 100,000 macrophages/cm 2 showed a functional barrier (transepithelial electrical resistance 〉 500ω.cm 2 ). This cell model was combined with the Pharmaceutical Aerosol Deposition Device on Cell Cultures. The functionality of the in vitro system was investigated with spray-dried fluorescently labelled poly(lactic-co-glycolic) acid particles loaded with ovalbumin as a model drug.

Type of Medium: Online Resource

ISSN: 0261-1929 , 2632-3559

URL: Article

DOI: 10.1177/026119291604400404

Language: English

Publisher: SAGE Publications

Publication Date: 2016

detail.hit.zdb_id: 2390905-5

SSG: 12,22

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Lipobiotin-capture magnetic bead assay for isolation, enrichment and detection of Mycobacterium tuberculosis from saliva

Hansen, Julia ; Kolbe, Katharina ; König, Inke R. ; [et al.]

Public Library of Science (PLoS) ; 2022

In: PLOS ONE Vol. 17, No. 7 ( 2022-7-15), p. e0265554-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-15), p. e0265554-

Abstract: Pulmonary Tuberculosis (TB) is diagnosed through sputum samples. As sputum sampling is challenging in children and cachexic patients, the development of diagnostic tests using saliva appears promising but has been discouraged due to low bacterial load and poor sensitivity. Here, we present a novel and rapid method to enrich Mycobacterium tuberculosis (Mtb) from saliva, which may serve as a basis for a diagnostic saliva test. Methods Lipobiotin-functionalized magnetic beads (LMBs) were incubated with Mtb-spiked PBS and saliva from healthy donors as well as with saliva from TB patients. Flow cytometry was used to evaluate the capacity of the beads to bind Mtb, while real-time quantitative polymerase chain reaction (qPCR) was utilized to detect Mtb and determine the amount of mycobacterial DNA in different sample types. Results We found that LMBs bind Mtb efficiently when compared to non-functionalized beads. The development of an qPCR assay based on the use of LMBs (LMB assay) allowed us to enrich mycobacterial DNA in spiked sample types, including PBS and saliva from healthy donors (enrichment of up to ~8.7 fold). In Mtb-spiked saliva samples, we found that the LMB assay improved the detection rate of 10 2 bacteria in a volume of 5 ml from 0 out of 15 (0%) to 6 out of 15 (40%). Consistent with that, the LMB assay increased the rate of correctly identified saliva samples from TB patients in two independent cohorts. Conclusions Implementation of the principle of the LMB-based assay may improve the sensitivity of existing diagnostic techniques, e.g. by functionalizing materials that facilitate Mtb sampling from the oral cavity.

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0265554

DOI: 10.1371/journal.pone.0265554.g001

DOI: 10.1371/journal.pone.0265554.g002

DOI: 10.1371/journal.pone.0265554.g003

DOI: 10.1371/journal.pone.0265554.g004

DOI: 10.1371/journal.pone.0265554.g005

DOI: 10.1371/journal.pone.0265554.g006

DOI: 10.1371/journal.pone.0265554.t001

DOI: 10.1371/journal.pone.0265554.s001

DOI: 10.1371/journal.pone.0265554.s002

DOI: 10.1371/journal.pone.0265554.s003

DOI: 10.1371/journal.pone.0265554.s004

DOI: 10.1371/journal.pone.0265554.s005

DOI: 10.1371/journal.pone.0265554.s006

DOI: 10.1371/journal.pone.0265554.s007

DOI: 10.1371/journal.pone.0265554.s008

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2022

detail.hit.zdb_id: 2267670-3

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Nasal delivery as a strategy for the prevention and treatment of COVID-19

Sonvico, Fabio ; Colombo, Gaia ; Quarta, Eride ; [et al.]

Informa UK Limited ; 2023

In: Expert Opinion on Drug Delivery

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In: Expert Opinion on Drug Delivery, Informa UK Limited

Type of Medium: Online Resource

ISSN: 1742-5247 , 1744-7593

URL: Article

DOI: 10.1080/17425247.2023.2263363

Language: English

Publisher: Informa UK Limited

Publication Date: 2023

detail.hit.zdb_id: 2232223-1

SSG: 15,3

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Intrathecal application of ethosuximide is highly efficient in suppressing seizures in a genetic model of absence epilepsy

Buschhoff, Anna-Sophia ; Scherließ, Regina ; de Mooij-van Malsen, Johanne G. ; [et al.]

Elsevier BV ; 2022

In: Epilepsy Research Vol. 184 ( 2022-08), p. 106967-

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In: Epilepsy Research, Elsevier BV, Vol. 184 ( 2022-08), p. 106967-

Type of Medium: Online Resource

ISSN: 0920-1211

URL: Article

DOI: 10.1016/j.eplepsyres.2022.106967

Language: English

Publisher: Elsevier BV

Publication Date: 2022

detail.hit.zdb_id: 2013048-X

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