In:
Annals of the New York Academy of Sciences, Wiley, Vol. 865, No. 1 ( 1998-12), p. 141-146
Abstract:
A bstract : In this study, the secretory effects of PACAP and PACAP analogues on [ 3 H]serotonin‐loaded purified rat peritoneal mast cells (RPMCs) were investigated. PACAP(1‐27) and PACAP(6‐27) stimulated [ 3 H]serotonin release with low potency (ED 50 : 2 × 10 −6 M) but high efficacy. The N‐terminally truncated PACAP form, PACAP(6‐27), stimulated tracer release with an ED 50 of 0.2 × 10 −6 M, indicating a high‐affinity PACAP receptor‐independent mechanism of action. The secretory response to PACAP(1‐27) could be inhibited by 60‐min preincubation with pertussis toxin (ptx), which inhibits G proteins. U73122, a cell‐permeable phospholipase C inhibitor, dose‐dependently inhibited the secretory effect of 5 μM PACAP(1‐27) with an IC 50 value of 4 μM ( N = 4; p 〉 0.006 ). We conclude that PACAP exerts a secretory effect in RPMCs by high‐affinity PACAP receptor‐independent direct activation of one or more G proteins, which may then activate the PLC‐dependent signal‐transduction pathway.
Type of Medium:
Online Resource
ISSN:
0077-8923
,
1749-6632
DOI:
10.1111/nyas.1998.865.issue-1
DOI:
10.1111/j.1749-6632.1998.tb11172.x
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
2834079-6
detail.hit.zdb_id:
211003-9
detail.hit.zdb_id:
2071584-5
SSG:
11
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