In:
European Journal of Biochemistry, Wiley, Vol. 143, No. 3 ( 1984-09), p. 613-620
Abstract:
Procedures for the stepwise addition of one or more deoxyribonucleotide residues to the 3′ end of an oligodeoxyribonucleoside phosphate acceptor using commercially available terminal deoxynucleotidyl transferase is described. 2–80 nmol of acceptors with a chain length of four, five or nine monomer units were elongated with a single 2′‐deoxyribonucleoside 5′‐triphosphate in yields of 20–30%. The monomers carried no protecting groups and were used both radioactively labelled and unlabelled. The elongated oligodeoxynucleoside phosphates were isolated by reverse‐phase (Nucleosil C 18 ) high‐performance liquid chromatography or paper chromatography. The isolated products were sequenced by the fingerprint method. Advantages and disadvantages of this new methodology for the enzymatic synthesis of defined oligodeoxynucleotides are discussed.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1984.143.issue-3
DOI:
10.1111/j.1432-1033.1984.tb08414.x
Language:
English
Publisher:
Wiley
Publication Date:
1984
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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