In:
The Journal of Immunology, The American Association of Immunologists, Vol. 175, No. 3 ( 2005-08-01), p. 1389-1398
Abstract:
Intestinal macrophages (IMAC) are a central component in the defense of the intestinal mucosa against luminal microbes. In normal mucosa, monocytes differentiate to immunologically tolerant IMAC with a typical phenotype lacking activation markers such as CD14 and TLRs 2 and 4. CD33+ IMAC were isolated from normal intestinal mucosa by immunomagnetic beads. A subtractive hybridization subtracting mRNA from normal IMAC from those of in vitro differentiated macrophages was performed. IMAC differentiation was studied in multicellular spheroids (MCS). Functional assays on migration of CD45R0+ T cells were performed in MCS coculture models. Of 76 clones, 3 obtained by subtractive mRNA hybridization showed & gt;99% homology to mRNA of MIP-3α, indicating that this chemokine is induced in IMAC compared with in vitro differentiated macrophages. MIP-3α protein expression was confirmed in cryostat sections of normal intestinal mucosa by immunohistochemistry. IMAC in the lamina propria stained positive for MIP-3α. FACS of purified IMAC clearly indicated expression of MIP-3α in these cells. In the MCS-in vitro differentiation model for IMAC, MIP-3α protein expression was absent on day 1 but detectable on day 7 of coculture, demonstrating the induction of MIP-3α during differentiation of IMAC. IMAC attracted CD45R0+ T cells to migrate into an MCS coculture model. In human mucosa, a close contact between IMAC and CD45R0+ T cells could be demonstrated. MIP-3α is induced during the differentiation of monocytes into IMAC. Our data suggest that MIP-3α expression could be involved in the recruitment of CD45R0+ cells into the lamina propria.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.175.3.1389
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2005
detail.hit.zdb_id:
1475085-5
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