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Eintauchen in den Sequenzraum der Thiolacton‐Präzisionspolymere: eine kombinatorische Strategie zur Identifizierung funktionaler Domänen

Celasun, Sensu ; Remmler, Dario ; Schwaar, Timm ; [et al.]

Wiley ; 2019

In: Angewandte Chemie Vol. 131, No. 7 ( 2019-02-11), p. 1980-1984

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In: Angewandte Chemie, Wiley, Vol. 131, No. 7 ( 2019-02-11), p. 1980-1984

Abstract: Funktionale Sequenzen, die mit m ‐THPC, einem Photosensibilisator der zweiten Generation, spezifisch wechselwirken, werden gezielt aus einer kombinatorischen Thiolacton/Michael‐Oligomer‐Bibliothek selektiert. Die Sequenzierung erfolgt mittels MALDI‐TOF MS/MS und liefert Bindedomänen, die als Teil von Tla/Michael‐PEG‐Konjugaten m ‐THPC effektiv binden und solubilisieren. Die Beladungskapazitäten und die Wirkstofffreisetzungskinetiken können dabei durch Variation des Bindesegments justiert werden.

Type of Medium: Online Resource

ISSN: 0044-8249 , 1521-3757

URL: Issue

URL: Article

DOI: 10.1002/ange.v131.7

DOI: 10.1002/ange.201810393

RVK:

VA 2100

RVK:

VN 5020

Language: English

Publisher: Wiley

Publication Date: 2019

detail.hit.zdb_id: 505868-5

detail.hit.zdb_id: 506609-8

detail.hit.zdb_id: 514305-6

detail.hit.zdb_id: 505872-7

detail.hit.zdb_id: 1479266-7

detail.hit.zdb_id: 505867-3

detail.hit.zdb_id: 506259-7

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Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Tscheuschner, Georg ; Ponader, Marco ; Raab, Christopher ; [et al.]

MDPI AG ; 2023

In: Viruses Vol. 15, No. 3 ( 2023-03-07), p. 697-

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In: Viruses, MDPI AG, Vol. 15, No. 3 ( 2023-03-07), p. 697-

Abstract: The cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses are key steps. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of the CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. The protocol involves precipitation with PEG 8000, followed by affinity extraction using a novel peptide aptamer. The efficiency of the protocol was validated using size exclusion chromatography, MALDI-TOF mass spectrometry, reversed-phase HPLC, and sandwich immunoassay. Furthermore, it was demonstrated that the final eluate of the affinity column is of exceptional purity (98.4%) determined by HPLC and detection at 220 nm. The scale-up of our proposed method seems to be straightforward, which opens the way to the large-scale production of such nanomaterials. This highly improved protocol may facilitate the use and implementation of plant viruses as nanotechnological platforms for in vitro and in vivo applications.

Type of Medium: Online Resource

ISSN: 1999-4915

URL: Article

DOI: 10.3390/v15030697

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2516098-9

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Efficient Screening of Combinatorial Peptide Libraries by Spatially Ordered Beads Immobilized on Conventional Glass Slides

Schwaar, Timm ; Lettow, Maike ; Remmler, Dario ; [et al.]

MDPI AG ; 2019

In: High-Throughput Vol. 8, No. 2 ( 2019-04-30), p. 11-

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In: High-Throughput, MDPI AG, Vol. 8, No. 2 ( 2019-04-30), p. 11-

Abstract: Screening of one-bead-one-compound (OBOC) libraries is a proven procedure for the identification of protein-binding ligands. The demand for binders with high affinity and specificity towards various targets has surged in the biomedical and pharmaceutical field in recent years. The traditional peptide screening involves tedious steps such as affinity selection, bead picking, sequencing, and characterization. Herein, we present a high-throughput “all-on-one chip” system to avoid slow and technically complex bead picking steps. On a traditional glass slide provided with an electrically conductive tape, beads of a combinatorial peptide library are aligned and immobilized by application of a precision sieve. Subsequently, the chip is incubated with a fluorophore-labeled target protein. In a fluorescence scan followed by matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry, high-affinity binders are directly and unambiguously sequenced with high accuracy without picking of the positive beads. The use of an optimized ladder sequencing approach improved the accuracy of the de-novo sequencing step to nearly 100%. The new technique was validated by employing a FLAG-based model system, identifying new peptide binders for the monoclonal M2 anti-FLAG antibody, and was finally utilized to search for IgG-binding peptides. In the present format, more than 30,000 beads can be screened on one slide.

Type of Medium: Online Resource

ISSN: 2571-5135

URL: Article

DOI: 10.3390/ht8020011

Language: English

Publisher: MDPI AG

Publication Date: 2019

detail.hit.zdb_id: 3056390-2

detail.hit.zdb_id: 3029030-2

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Comparison of the fragmentation behavior of DNA and LNA single strands and duplexes

Ickert, Stefanie ; Schwaar, Timm ; Springer, Andreas ; [et al.]

Wiley ; 2019

In: Journal of Mass Spectrometry Vol. 54, No. 5 ( 2019-05), p. 402-411

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In: Journal of Mass Spectrometry, Wiley, Vol. 54, No. 5 ( 2019-05), p. 402-411

Abstract: DNA and locked nucleic acid (LNA) were characterized as single strands, as well as double stranded DNA‐DNA duplexes and DNA‐LNA hybrids using tandem mass spectrometry with collision‐induced dissociation. Additionally, ion mobility spectrometry was carried out on selected species. Oligonucleotide duplexes of different sequences — bearing mismatch positions and abasic sites of complementary DNA 15‐mers — were investigated to unravel general trends in their stability in the gas phase. Single‐stranded LNA oligonucleotides were also investigated with respect to their gas phase behavior and fragmentation upon collision‐induced dissociation. In contrast to the collision‐induced dissociation of DNA, almost no base loss was observed for LNAs. Here, backbone cleavages were the dominant dissociation pathways. This finding was further underlined by the need for higher activation energies. Base losses from the LNA strand were also absent in fragmentation experiments of the investigated DNA‐LNA hybrid duplexes. While DNA‐DNA duplexes dissociated easily into single stranded fragments, the high stability of DNA‐LNA hybrids resulted in predominant fragmentation of the DNA part rather than the LNA, while base losses were only observed from the DNA single strand of the hybrid.

Type of Medium: Online Resource

ISSN: 1076-5174 , 1096-9888

URL: Issue

URL: Article

DOI: 10.1002/jms.v54.5

DOI: 10.1002/jms.4344

Language: English

Publisher: Wiley

Publication Date: 2019

detail.hit.zdb_id: 2197367-2

detail.hit.zdb_id: 1472468-6

detail.hit.zdb_id: 7414-7

SSG: 11

SSG: 12

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Fast Confirmation of Antibody Identity by MALDI-TOF MS Fingerprints

Tscheuschner, Georg ; Schwaar, Timm ; Weller, Michael G.

MDPI AG ; 2020

In: Antibodies Vol. 9, No. 2 ( 2020-03-26), p. 8-

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In: Antibodies, MDPI AG, Vol. 9, No. 2 ( 2020-03-26), p. 8-

Abstract: Thousands of antibodies for diagnostic and other analytical purposes are on the market. However, it is often difficult to identify duplicates, reagent changes, and to assign the correct original publications to an antibody. This slows down scientific progress and might even be a cause of irreproducible research and a waste of resources. Recently, activities were started to suggest the sole use of recombinant antibodies in combination with the open communication of their sequence. In this case, such uncertainties should be eliminated. Unfortunately, this approach seems to be rather a long-term vision since the development and manufacturing of recombinant antibodies remain quite expensive in the foreseeable future. Nearly all commercial antibody suppliers also may be reluctant to publish the sequence of their antibodies, since they fear counterfeiting. De novo sequencing of antibodies is also not feasible today for a reagent user without access to the hybridoma clone. Nevertheless, it seems to be crucial for any scientist to have the opportunity to identify an antibody undoubtedly to guarantee the traceability of any research activity using antibodies from a third party as a tool. For this purpose, we developed a method for the identification of antibodies based on a MALDI-TOF MS fingerprint. To circumvent lengthy denaturation, reduction, alkylation, and enzymatic digestion steps, the fragmentation was performed with a simple formic acid hydrolysis step. Eighty-nine unknown monoclonal antibodies were used for this study to examine the feasibility of this approach. Although the molecular assignment of peaks was rarely possible, antibodies could be easily recognized in a blinded test, simply from their mass-spectral fingerprint. A general protocol is given, which could be used without any optimization to generate fingerprints for a database. We want to propose that, in most scientific projects relying critically on antibody reagents, such a fingerprint should be established to prove and document the identity of the used antibodies, as well as to assign a specific reagent to a datasheet of a commercial supplier, public database record, or antibody ID.

Type of Medium: Online Resource

ISSN: 2073-4468

URL: Article

DOI: 10.3390/antib9020008

Language: English

Publisher: MDPI AG

Publication Date: 2020

detail.hit.zdb_id: 2661514-9

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A photoswitchable catalyst system for remote-controlled (co)polymerization in situ

Eisenreich, Fabian ; Kathan, Michael ; Dallmann, Andre ; [et al.]

Springer Science and Business Media LLC ; 2018

In: Nature Catalysis Vol. 1, No. 7 ( 2018-07-02), p. 516-522

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In: Nature Catalysis, Springer Science and Business Media LLC, Vol. 1, No. 7 ( 2018-07-02), p. 516-522

Type of Medium: Online Resource

ISSN: 2520-1158

URL: Article

DOI: 10.1038/s41929-018-0091-8

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2018

detail.hit.zdb_id: 2916779-6

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Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies

Dippong, Martin ; Carl, Peter ; Lenz, Christine ; [et al.]

American Chemical Society (ACS) ; 2017

In: Analytical Chemistry Vol. 89, No. 7 ( 2017-04-04), p. 4007-4012

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In: Analytical Chemistry, American Chemical Society (ACS), Vol. 89, No. 7 ( 2017-04-04), p. 4007-4012

Type of Medium: Online Resource

ISSN: 0003-2700 , 1520-6882

URL: Article

DOI: 10.1021/acs.analchem.6b04569

DOI: 10.1021/acs.analchem.6b04569.s001

Language: English

Publisher: American Chemical Society (ACS)

Publication Date: 2017

detail.hit.zdb_id: 1483443-1

detail.hit.zdb_id: 1508-8

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Predictable Peptide Conjugation Ratios by Activation of Proteins with Succinimidyl Iodoacetate (SIA)

Abbas, Ioana ; Schwaar, Timm ; Bienwald, Frank ; [et al.]

MDPI AG ; 2017

In: Methods and Protocols Vol. 1, No. 1 ( 2017-09-25), p. 2-

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In: Methods and Protocols, MDPI AG, Vol. 1, No. 1 ( 2017-09-25), p. 2-

Type of Medium: Online Resource

ISSN: 2409-9279

URL: Article

DOI: 10.3390/mps1010002

Language: English

Publisher: MDPI AG

Publication Date: 2017

detail.hit.zdb_id: 2934611-3

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Specific Drug Formulation Additives: Revealing the Impact of Architecture and Block Length Ratio

Wieczorek, Sebastian ; Schwaar, Timm ; Senge, Mathias O. ; [et al.]

American Chemical Society (ACS) ; 2015

In: Biomacromolecules Vol. 16, No. 10 ( 2015-10-12), p. 3308-3312

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In: Biomacromolecules, American Chemical Society (ACS), Vol. 16, No. 10 ( 2015-10-12), p. 3308-3312

Type of Medium: Online Resource

ISSN: 1525-7797 , 1526-4602

URL: Article

DOI: 10.1021/acs.biomac.5b00961

Language: English

Publisher: American Chemical Society (ACS)

Publication Date: 2015

detail.hit.zdb_id: 2006291-6

SSG: 12

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Digging into the Sequential Space of Thiolactone Precision Polymers: A Combinatorial Strategy to Identify Functional Domains

Celasun, Sensu ; Remmler, Dario ; Schwaar, Timm ; [et al.]

Wiley ; 2019

In: Angewandte Chemie International Edition Vol. 58, No. 7 ( 2019-02-11), p. 1960-1964

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In: Angewandte Chemie International Edition, Wiley, Vol. 58, No. 7 ( 2019-02-11), p. 1960-1964

Abstract: Functional sequences of precision polymers based on thiolactone/Michael chemistry are identified from a large one‐bead one‐compound library. Single‐bead readout by MALDI‐TOF MS/MS identifies sequences that host m ‐THPC that is a second generation photo‐sensitizer drug. The corresponding Tla/Michael‐PEG conjugates make m ‐THPC available in solution and drug payload as well as drug release kinetics can be fine‐tuned by the precision segment.

Type of Medium: Online Resource

ISSN: 1433-7851 , 1521-3773

URL: Issue

URL: Article

DOI: 10.1002/anie.v58.7

DOI: 10.1002/anie.201810393

RVK:

VA 2100

Language: English

Publisher: Wiley

Publication Date: 2019

detail.hit.zdb_id: 2011836-3

detail.hit.zdb_id: 123227-7

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