In:
Journal of Cellular and Molecular Medicine, Wiley, Vol. 18, No. 8 ( 2014-08), p. 1631-1643
Abstract:
Cystic fibrosis ( CF ) is caused by mutations in the CF transmembrane conductance regulator ( CFTR ) gene, with most of the mortality given by the lung disease. Human amniotic mesenchymal stromal (stem) cells (h AMSC s) hold great promise for regenerative medicine in the field of lung disease; however, their potential as therapeutics for CF lung disease has not been fully explored. In the present study, h AMSC s were analysed in co‐cultures on Transwell filters with CF immortalized airway epithelial cells ( CFBE 41o‐ line) at different ratios to exploit their potency to resume basic defects associated with CF . The results show that F‐actin content was increased in co‐cultures as compared with CF cells and actin was reorganized to form stress fibres. Confocal microscopy studies revealed that co‐cultures had a tendency of increased expression of occludin and ZO ‐1 at the intercellular borders, paralleled by a decrease in dextran permeability, suggestive of more organized tight junctions ( TJ s). Spectrofluorometric analysis of CFTR function demonstrated that h AMSC ‐ CFBE co‐cultures resumed chloride transport, in line with the appearance of the mature Band C of CFTR protein by W estern blotting. Moreover, hAMSC ‐ CFBE co‐cultures, at a 1:5 ratio, showed a decrease in fluid absorption, as opposed to CFBE cell monolayers that displayed a great rate of fluid resorption from the apical side. Our data show that human amniotic MSC s can be used in co‐culture with CF respiratory epithelial cells to model their engraftment into the airways and have the potential to resume a tight epithelium with partial correction of the CF phenotype.
Type of Medium:
Online Resource
ISSN:
1582-1838
,
1582-4934
DOI:
10.1111/jcmm.2014.18.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2076114-4
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