In:
FEBS Letters, Wiley, Vol. 398, No. 1 ( 1996-11-25), p. 120-124
Abstract:
Activins are members of the TGF‐β superfamily and are classified into 3 types: activin A, which consists of a homodimer of β A , activin B, which consists of a homodimer of β B , and activin AB, which consists of a heterodimer of β A β B . We studied the expression of activin mRNAs by RT‐PCR in normal human epidermis, cultured keratinocytes, and DJM‐1 cells (a squamous cell carcinoma line). We could detect only activin A mRNA ( β A ) in normal human epidermis. In cultured keratinocyte and DJM‐1 cells, activin β A mRNA was observed at 4 h but not at 96 h after plating. Activin A activity was detected in the conditioned medium of DJM‐1 cells within 48 h. In addition, although follistatin mRNA was not observed in human epidermis in situ, it was transiently expressed in cultured cells at 4 h after plating. These findings suggest that the expression of these molecules in keratinocytes is associated with cell proliferation. In an in vitro tissue injury model, activin A was observed at the wound edge, where cell migration and proliferation may be activated. In DJM‐1 cells cultured for 92 h, β A mRNA was observed 4 h after injury treatment. These findings suggest that activin A acts as a potent inducer of proliferation in vitro, at least in keratinocytes.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(96)01221-5
Language:
English
Publisher:
Wiley
Publication Date:
1996
detail.hit.zdb_id:
1460391-3
SSG:
12
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