In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 9 ( 2021-9-8), p. e3001385-
Abstract:
Intrauterine infection/inflammation (IUI) is a major contributor to preterm labor (PTL). However, IUI does not invariably cause PTL. We hypothesized that quantitative and qualitative differences in immune response exist in subjects with or without PTL. To define the triggers for PTL, we developed rhesus macaque models of IUI driven by lipopolysaccharide (LPS) or live Escherichia coli . PTL did not occur in LPS challenged rhesus macaques, while E . coli– infected animals frequently delivered preterm. Although LPS and live E . coli both caused immune cell infiltration, E . coli– infected animals showed higher levels of inflammatory mediators, particularly interleukin 6 (IL-6) and prostaglandins, in the chorioamnion-decidua and amniotic fluid (AF). Neutrophil infiltration in the chorio-decidua was a common feature to both LPS and E . coli . However, neutrophilic infiltration and IL6 and PTGS2 expression in the amnion was specifically induced by live E . coli . RNA sequencing (RNA-seq) analysis of fetal membranes revealed that specific pathways involved in augmentation of inflammation including type I interferon (IFN) response, chemotaxis, sumoylation, and iron homeostasis were up-regulated in the E . coli group compared to the LPS group. Our data suggest that the intensity of the host immune response to IUI may determine susceptibility to PTL.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001385
DOI:
10.1371/journal.pbio.3001385.g001
DOI:
10.1371/journal.pbio.3001385.g002
DOI:
10.1371/journal.pbio.3001385.g003
DOI:
10.1371/journal.pbio.3001385.g004
DOI:
10.1371/journal.pbio.3001385.g005
DOI:
10.1371/journal.pbio.3001385.g006
DOI:
10.1371/journal.pbio.3001385.g007
DOI:
10.1371/journal.pbio.3001385.g008
DOI:
10.1371/journal.pbio.3001385.t001
DOI:
10.1371/journal.pbio.3001385.t002
DOI:
10.1371/journal.pbio.3001385.s001
DOI:
10.1371/journal.pbio.3001385.s002
DOI:
10.1371/journal.pbio.3001385.s003
DOI:
10.1371/journal.pbio.3001385.s004
DOI:
10.1371/journal.pbio.3001385.s005
DOI:
10.1371/journal.pbio.3001385.s006
DOI:
10.1371/journal.pbio.3001385.s007
DOI:
10.1371/journal.pbio.3001385.s008
DOI:
10.1371/journal.pbio.3001385.s009
DOI:
10.1371/journal.pbio.3001385.s010
DOI:
10.1371/journal.pbio.3001385.s011
DOI:
10.1371/journal.pbio.3001385.s012
DOI:
10.1371/journal.pbio.3001385.s013
DOI:
10.1371/journal.pbio.3001385.s014
DOI:
10.1371/journal.pbio.3001385.r001
DOI:
10.1371/journal.pbio.3001385.r002
DOI:
10.1371/journal.pbio.3001385.r003
DOI:
10.1371/journal.pbio.3001385.r004
DOI:
10.1371/journal.pbio.3001385.r005
DOI:
10.1371/journal.pbio.3001385.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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