In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-06-01)
Abstract:
Regulation of intracellular Ca 2+ signaling is a major determinant of CD8 + T cell responsiveness, but the mechanisms underlying this regulation of Ca 2+ levels, especially in naïve CD8 + T cells, are not fully defined. Here, we showed that microRNA-150 (miR-150) controls intracellular Ca 2+ levels in naïve CD8 + T cells required for activation by suppressing TMEM20, a negative regulator of Ca 2+ extrusion. miR-150 deficiency increased TMEM20 expression, which resulted in increased intracellular Ca 2+ levels in naïve CD8 + T cells. The subsequent increase in Ca 2+ levels induced expression of anergy-inducing genes, such as Cbl-b , Egr2 , and p27 , through activation of NFAT1, as well as reduced cell proliferation, cytokine production, and the antitumor activity of CD8 + T cells upon antigenic stimulation. The anergy-promoting molecular milieu and function induced by miR-150 deficiency were rescued by reinstatement of miR-150. Additionally, knockdown of TMEM20 in miR-150-deficient naïve CD8 + T cells reduced intracellular Ca 2+ levels. Our findings revealed that miR-150 play essential roles in controlling intracellular Ca 2+ level and activation in naïve CD8 + T cells, which suggest a mechanism to overcome anergy induction by the regulation of intracellular Ca 2+ levels.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-017-02697-x
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2017
detail.hit.zdb_id:
2615211-3
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