In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_1 ( 2014-01-01), p. 16-20
Abstract:
A taxonomic study was carried out on strain 22II14-10F7 T , which was isolated from the deep-sea water of the Atlantic Ocean with oil-degrading enrichment. The bacterium was Gram-stain-negative, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinities from 0.5 to 15 % and at temperatures from 4 to 37 °C; it was unable to hydrolyse Tween 40, 80 or gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II14-10F7 T represented a member of the genus Zunongwangia , with highest sequence similarity of 97.3 % to Zunongwangia profunda SM-A87 T , while the similarities to other species were all below 94.0 %. The DNA–DNA hybridization estimate of the similarity between strain 22II14-10F7 T and Z. profunda SM-A87 T was 27.20±2.43 % according to their genome sequences. The principal fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 15 : 1 G, iso-C 17 : 0 3-OH, summed feature 3 (C 16 : 1 ω7 c /ω6 c ) and summed feature 9 (iso-C 17 : 1 ω9 c or C 16 : 0 10-methyl). The G+C content of the chromosomal DNA was 35.5 mol%. The major respiratory quinone was determined to be MK-6. Phosphatidylethanolamine (PE), two aminolipids (AL1 and AL2) and five unknown lipids (L1–L5) were present. The combined genotypic and phenotypic data show that strain 22II14-10F7 T represents a novel species of the genus Zunongwangia , for which the name Zunongwangia atlantica sp. nov. is proposed, with the type strain 22II14-10F7 T ( = CGMCC1.12470 T = LMG 27421 T = MCCC 1A06481 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.054007-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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