In:
FEBS Letters, Wiley, Vol. 591, No. 9 ( 2017-05), p. 1258-1265
Abstract:
The N‐terminal extension ( NTE ) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B‐type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C‐D ring methine bridge and the surrounding hydrogen bonding network in the physiologically active Pfr state. These changes are accompanied by a shift of the conformational equilibrium between two Pfr substates, which might affect the thermal isomerization rate of the C‐D double bond and, thus, account for the effect of the NTE on the dark reversion kinetics.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1002/feb2.2017.591.issue-9
DOI:
10.1002/1873-3468.12642
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
1460391-3
SSG:
12
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