In:
Pigment Cell & Melanoma Research, Wiley, Vol. 29, No. 3 ( 2016-05), p. 309-316
Abstract:
Rhododendrol ( RD ) is a potent tyrosinase inhibitor that is metabolized to RD ‐quinone by tyrosinase, which may underlie the cytotoxicity of RD and leukoderma of the skin that may result. We have examined how forced expression of the NAD (P)H quinone dehydrogenase, quinone 1 ( NQO 1), a major quinone‐reducing enzyme in cytosol, affects the survival of RD ‐treated cells. We found that treatment of the mouse melanoma cell line B16 BL 6 or normal human melanocytes with carnosic acid, a transcriptional inducer of the NQO 1 gene, notably suppressed the cell killing effect of RD . This effect was mostly abolished by ES 936, a highly specific NQO 1 inhibitor. Moreover, conditional overexpression of the human NQO 1 transgene in B16 BL 6 led to an expression‐dependent increase of cell survival after RD treatment. Our results suggest that NQO 1 attenuates the cytotoxicity of RD and/or its metabolites.
Type of Medium:
Online Resource
ISSN:
1755-1471
,
1755-148X
DOI:
10.1111/pcmr.2016.29.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2425880-5
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