In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_10 ( 2014-10-01), p. 3427-3433
Abstract:
A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6 T , was isolated from the inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6 T grew at high salt concentrations [10 % (w/v) NaCl], temperatures of 15–41 °C and a pH range of 5–12. It showed highest (99.7 %) 16S rRNA gene sequence similarity with Micrococcus yunnanensis YIM 65004 T followed by Micrococcus luteus NCTC 2665 T (99.6 %) and Micrococcus endophyticus YIM 56238 T (99.0 %). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to M. yunnanensis YIM 65004 T and M. luteus NCTC 2665 T . Like other members of the genus Micrococcus , strain AE-6 T had a high content of branched chain fatty acids (iso-C15 : 0 and anteiso-C15 : 0). MK-8(H 2 ) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an ‘A2 l -Lys-peptide subunit’ type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA–DNA hybridization of AE-6 T with its closest relatives from the genus Micrococcus produced a value of less than 70%. Based on the results of this study, strain AE-6 T could be clearly differentiated from other members of the genus Micrococcus . We propose that it represents a novel species of the genus Micrococcus and suggest the name Micrococcus aloeverae sp. nov., with strain AE-6 T ( = MCC 2184 T = DSM 27472 T ) as the type strain of the species.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.063339-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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