In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2023-2-24), p. e0279652-
Abstract:
Extracellular vesicles (EVs) are cell-derived membrane vesicles which play an important role in cell-to-cell communication and physiology. EVs deliver biological information from producing to recipient cells by transport of different cargo such as proteins, mRNAs, microRNAs, non-coding RNAs and lipids. Adipose tissue EVs could regulate metabolic and inflammatory interactions inside adipose tissue depots as well as distal tissues. Thus, adipose tissue EVs are assumed to be implicated in obesity-associated pathologies, notably in insulin resistance and type 2 diabetes mellitus (T2DM). In this study we for the first time characterize EVs secreted by visceral (VAT) and subcutaneous adipose tissue (SAT) of patients with obesity and T2DM with standard methods as well as analyze their morphology with cryo-electron microscopy. Cryo-electron microscopy allowed us to visualize heterogeneous population of EVs of various size and morphology including single EVs and EVs with internal membrane structures in samples from obese patients as well from the control group. Single vesicles prevailed (up to 85% for SAT, up to 75% for VAT) and higher proportion of EVs with internal membrane structures compared to SAT was typical for VAT. Decreased size of single and double SAT EVs compared to VAT EVs, large proportion of multilayered EVs and all EVs with internal membrane structures secreted by VAT distinguished obese patients with/without T2DM from the control group. These findings could support the idea of modified biogenesis of EVs during obesity and T2DM.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0279652
DOI:
10.1371/journal.pone.0279652.g001
DOI:
10.1371/journal.pone.0279652.g002
DOI:
10.1371/journal.pone.0279652.g003
DOI:
10.1371/journal.pone.0279652.g004
DOI:
10.1371/journal.pone.0279652.g005
DOI:
10.1371/journal.pone.0279652.t001
DOI:
10.1371/journal.pone.0279652.t002
DOI:
10.1371/journal.pone.0279652.t003
DOI:
10.1371/journal.pone.0279652.s001
DOI:
10.1371/journal.pone.0279652.s002
DOI:
10.1371/journal.pone.0279652.s003
DOI:
10.1371/journal.pone.0279652.s004
DOI:
10.1371/journal.pone.0279652.s005
DOI:
10.1371/journal.pone.0279652.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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