In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-4), p. e0246313-
Abstract:
Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a liver cell line expressing HBV RNA at a low frequency. These genes included DOCK11 and DENND2A , which encode small GTPase regulators. In primary human hepatocytes infected with HBV, knockdown of these two genes decreased the amount of both HBV DNA and covalently closed circular DNA to below the limit of detection. Our findings reveal a role for DOCK11 and DENND2A in the maintenance of HBV.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0246313
DOI:
10.1371/journal.pone.0246313.g001
DOI:
10.1371/journal.pone.0246313.g002
DOI:
10.1371/journal.pone.0246313.g003
DOI:
10.1371/journal.pone.0246313.g004
DOI:
10.1371/journal.pone.0246313.g005
DOI:
10.1371/journal.pone.0246313.g006
DOI:
10.1371/journal.pone.0246313.t001
DOI:
10.1371/journal.pone.0246313.s001
DOI:
10.1371/journal.pone.0246313.s002
DOI:
10.1371/journal.pone.0246313.s003
DOI:
10.1371/journal.pone.0246313.s004
DOI:
10.1371/journal.pone.0246313.s005
DOI:
10.1371/journal.pone.0246313.s006
DOI:
10.1371/journal.pone.0246313.s007
DOI:
10.1371/journal.pone.0246313.s008
DOI:
10.1371/journal.pone.0246313.s009
DOI:
10.1371/journal.pone.0246313.s010
DOI:
10.1371/journal.pone.0246313.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Bookmarklink