In:
European Journal of Biochemistry, Wiley, Vol. 235, No. 1-2 ( 1996-01), p. 275-280
Abstract:
Chromogranin A is a protein contained in the secretory granules of many neuroendocrine cells. The linear antigenic sites of human chromogranin A were studied by examining the cross‐reaction of polyclonal and monoclonal anti‐chromogranin A antibodies with native chromogranin A and with synthetic peptides encompassing most of the chromogranin A sequence. Chromogranin A residues 1–20, 47–67, 107–158, 254–297, 331–375, and 395–419 were found to be poorly or not antigenic, while residues 25–46, 163–210, 231–253, 298–314 and 68–106, 222–230, 315–330, 376–394 were found to contain weak and strong antigenic sites, respectively. Residues 68–70 (GAK) and 81–90 (GFEDELSEVL) were strongly recognized by two mouse mAbs (B4E11 and A11, respectively). Since mAb A11 has been previously used for immunohistochemical analysis of chromogranin‐A‐producing tissues from different species and for in vivo imaging of chromogranin‐A‐positive endocrine tumors, these results imply that at least part of the 81–90 region is surface‐exposed in cryostat tissue sections as well as in vivo. The results may help in selecting new antibodies with improved affinity and immunogenicity for in vivo targeting of chromogranin‐A‐producing tumors.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1996.235.issue-1-2
DOI:
10.1111/j.1432-1033.1996.00275.x
Language:
English
Publisher:
Wiley
Publication Date:
1996
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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