In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2022-8-11), p. e0272903-
Abstract:
Mycoplasma hyorhinis is an emerging swine pathogen bacterium causing polyserositis and polyarthritis in weaners and finishers. The pathogen is distributed world-wide, generating significant economic losses. No commercially available vaccine is available in Europe. Therefore, besides improving the housing conditions for prevention, antimicrobial therapy of the diseased animals is the only option to control the infection. Our aim was to determine the minimal inhibitory concentrations (MIC) of ten antimicrobials potentially used against M . hyorhinis infection. The antibiotic susceptibility of 76 M . hyorhinis isolates from Belgium, Germany, Hungary, Italy and Poland collected between 2019 and 2021 was determined by broth micro-dilution method and mismatch amplification mutation assay (MAMA). Low concentrations of tiamulin (MIC 90 0.312 μg/ml), doxycycline (MIC 90 0.078 μg/ml), oxytetracycline (MIC 90 0.25 μg/ml), florfenicol (MIC 90 2 μg/ml) and moderate concentrations of enrofloxacin (MIC 90 1.25 μg/ml) inhibited the growth of the isolates. For the tested macrolides and lincomycin, a bimodal MIC pattern was observed (MIC 90 〉 64 μg/ml for lincomycin, tulathromycin, tylosin and tilmicosin and 5 μg/ml for tylvalosin). The results of the MAMA assay were in line with the conventional method with three exceptions. Based on our statistical analyses, significant differences in MIC values of tiamulin and doxycycline were observed between certain countries. Our results show various levels of antimicrobial susceptibility among M . hyorhinis isolates to the tested antibiotics. The data underline the importance of susceptibility monitoring on pan-European level and provides essential information for proper antibiotic choice in therapy.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0272903
DOI:
10.1371/journal.pone.0272903.g001
DOI:
10.1371/journal.pone.0272903.g002
DOI:
10.1371/journal.pone.0272903.t001
DOI:
10.1371/journal.pone.0272903.t002
DOI:
10.1371/journal.pone.0272903.t003
DOI:
10.1371/journal.pone.0272903.t004
DOI:
10.1371/journal.pone.0272903.s001
DOI:
10.1371/journal.pone.0272903.s002
DOI:
10.1371/journal.pone.0272903.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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