In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-13), p. e0275860-
Abstract:
Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0275860
DOI:
10.1371/journal.pone.0275860.g001
DOI:
10.1371/journal.pone.0275860.g002
DOI:
10.1371/journal.pone.0275860.g003
DOI:
10.1371/journal.pone.0275860.g004
DOI:
10.1371/journal.pone.0275860.g005
DOI:
10.1371/journal.pone.0275860.g006
DOI:
10.1371/journal.pone.0275860.g007
DOI:
10.1371/journal.pone.0275860.t001
DOI:
10.1371/journal.pone.0275860.t002
DOI:
10.1371/journal.pone.0275860.s001
DOI:
10.1371/journal.pone.0275860.s002
DOI:
10.1371/journal.pone.0275860.s003
DOI:
10.1371/journal.pone.0275860.s004
DOI:
10.1371/journal.pone.0275860.s005
DOI:
10.1371/journal.pone.0275860.s006
DOI:
10.1371/journal.pone.0275860.s007
DOI:
10.1371/journal.pone.0275860.s008
DOI:
10.1371/journal.pone.0275860.s009
DOI:
10.1371/journal.pone.0275860.r001
DOI:
10.1371/journal.pone.0275860.r002
DOI:
10.1371/journal.pone.0275860.r003
DOI:
10.1371/journal.pone.0275860.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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