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  • 1
    Online Resource
    Online Resource
    CHANGES IN TURKEY SPERMATOZOZA MOTILITY PARAMETERS AFTER ADDITION OF COPPER SULPHATE IN VITRO
    Slovak University of Agriculture in Nitra ; 2015
    In:  Journal of microbiology, biotechnology and food sciences Vol. 4, No. special issue 2 (Biotechnology) ( 2015-02-02), p. 98-100
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 4, No. special issue 2 (Biotechnology) ( 2015-02-02), p. 98-100
    Abstract: Copper as essential trace element is obligatory in many biological processes. Nevertheless higher concentrations of this metal are highly toxic for organisms at different levels and of course for spermatozoa. The aim of present work was to examine impact of copper sulphate on turkey spermatozoa motility parameters (line Big 6) during in vitro incubation at 41°C. Four concentration of CuSO4: M1 – 6.25 μg.ml-1; M2 – 12.5 μg.ml-1; M3 – 25.0 μg.ml-1; M4 – 50.0 μg.ml-1 were investigated. The motility parameters were evaluated using the Computer Assisted Semen Analyzer system (program Sperm Vision®) at four time periods: 0, 30, 60 and 90 minutes. Immediately at the beginning of incubation significantly lower values of progressive motility were detected in samples M3 (p
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2015.4.special2.98-100
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2015
    detail.hit.zdb_id: 2641525-2
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  • 2
    Online Resource
    Online Resource
    ROLE OF NATURAL SUBSTANCES AND VITAMIN SUPPLEMENTATION IN TINNITUS PREVENTION AND TREATMENT
    Slovak University of Agriculture in Nitra ; 2016
    In:  Journal of microbiology, biotechnology and food sciences Vol. 6, No. 3 ( 2016-12-01), p. 987-994
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 6, No. 3 ( 2016-12-01), p. 987-994
    Abstract: The aim of this review is to refer a possibility of using natural substances for treating or reducing the symptoms of tinnitus. Tinnitus is a sensation of sound without an external source. It often manifests as a ringing in the ears, but it may also sound like a buzzing, hissing, whistling or even roaring in the head. Tinnitus is a symptom of an underlying condition. It can be linked to hearing loss, stress, ear damage, blood pressure, tumours and atherosclerosis. Exposure to loud noise is one major cause of tinnitus, since it wears down the delicate hair cells in inner ear that translate sounds into nerve impulses. Potential therapy of tinnitus is a pharmacological treatments. Fortunately, there are many effective natural alternatives to drugs that can bring considerable relief and help cope. The potential form of treatment is vitamins and natural flavonoids therapy. Low levels of melatonin and vitamin B12 in body have a significant correlation with the development of tinnitus. It was reported that melatonin is useful in the treatment of tinnitus, even in cases associated with sleep disturbance. There are relationship between vitamin B12 deficiency and dysfunction of the auditory pathway. Antioxidants are another substances which have a promising effect in the treatments of tinnitus. The constituents of G. biloba are potent scavengers of free radicals and has been prescribed their positive effect on treat of central nervous system disorders and cognitive deficits. Positive antioxidant effects have vitamin C, hesparidin and diosmin also.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2016/17.6.3.987-994
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2016
    detail.hit.zdb_id: 2641525-2
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  • 3
    Online Resource
    Online Resource
    IMPACT OF TILMICOSIN ON THE RABBIT SPERMATOZOA MOTILITY AND VIABILITY
    Slovak University of Agriculture in Nitra ; 2016
    In:  Journal of microbiology, biotechnology and food sciences Vol. 5, No. special 1 ( 2016-02-08), p. 53-56
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 5, No. special 1 ( 2016-02-08), p. 53-56
    Abstract: The aim of this study was to examine the effects of tilmicosin on rabbit spermatozoa viability and motility parameters during short-term in vitro incubation at 37°C. Semen samples were collected from adult New Zealand White rabbits and diluted with eight concentrations of tilmicosin: 0.300, 0.350, 0.400, 0.466, 0.500, 0.583, 0.600 and 0.700 mg.ml–1 (TIL 1 – 8) diluted in the physiological solution. The motility parameters were evaluated using the Computer Assisted Semen Analyzer system (Sperm Vision®) at five time periods: 0, 1, 2, 3 and 4 hours. Cell viability was measured using the metabolic activity MTT assay. Immediately at the beginning of incubation significantly higher values of motility (MOT) and progressive motility (PRO) were detected in samples TIL2, TIL5 and TIL7 compared to the control. Significantly lower values of tested motility parameters were observed after 1 and 3 hours of incubation. All concentrations of tilmicosin have no significantly negative effect on the parameters of the MOT, PRO and velocity curved line (VCL) after 4 hours of incubation in vitro at 37°C. Concentration of tilmicosin 0.350 mg.ml-1 has a positive impact on MOT (72.39 ± 15.62%, p
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2016.5.special1.53-56
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2016
    detail.hit.zdb_id: 2641525-2
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  • 4
    Online Resource
    Online Resource
    LOW TAURINE CONCENTRATIONS POSSITIVELY AFFECT RABBIT SPERMATOZOA PROPERTIES IN LATER TIME INTERVALS
    Slovak University of Agriculture in Nitra ; 2017
    In:  Journal of microbiology, biotechnology and food sciences Vol. 7, No. 2 ( 2017-10-01), p. 128-131
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 7, No. 2 ( 2017-10-01), p. 128-131
    Abstract: The aim of this study was to determinate the effect of various taurine concentrations on rabbit spermatozoa in in vitro conditions with the potential use in routine artificial insemination. Taurine, amino acid distinguished by its strong antioxidant properties, has been widely tested on spermatozoa of all kinds of farm animals except for the rabbits. This study investigated differences in rabbit spermatozoa motility and viability between the control sample and samples enriched with taurine solutions: TAU3 (3.125 mM), TAU6 (6.250 mM) and TAU12 (12.5 mM). Ejaculates of nine adult rabbits were subjected to this study and observed at time intervals 0, 90, 180 and 270 minutes while cultivated at 39°C. For detection of viability the MTT test was used and motility parameters were carried out using CASA analysis. Experimental samples were compared against the control. Assessment of the mitochondrial toxicity revealed that the addition of taurine ensured the higher viability in supplemented samples however with no statistical significance. Monitored motility parameters (motility, progressive motility and velocity curved line of spermatozoa) showed that taurine treated spermatozoa have lower activity at the beginning of the study but along with increasing time the positive effect of taurine was induced. Significant (P
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2017.7.2.128-131
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2017
    detail.hit.zdb_id: 2641525-2
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  • 5
    Online Resource
    Online Resource
    VISCUM ALBUM PINI EFFECT ON RABBIT SPERMATOZOA MOTILITY, VIABILITY, MEMBRANE AND ACROSOME INTEGRITY IN VITRO
    Slovak University of Agriculture in Nitra ; 2019
    In:  Journal of microbiology, biotechnology and food sciences Vol. 9, No. 1 ( 2019-08-01), p. 144-150
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 9, No. 1 ( 2019-08-01), p. 144-150
    Abstract: Mistletoe (Viscum album L.) was previously described as an effective additive in the therapy of patients with cancer after medical therapy of the primary neoplasia. The defence activity and also direct cytotoxic stimulation was found to be upstanding for the anti-carcinogenic action. The main target of this study was to detect the effect of Viscum album pini L. at various concentration 1.0 (PA); 0.66 (PB); 0.33 (PC); 0.1 (PD); 0.066 (PE) and 0.033 (PF) mg/mL on the fine rabbit spermatozoa motility parameters at various time periods (0; 1; 2 and 3 h) during the cultivation in vitro. The CASA methodology was applied to estimate the spermatozoa motility parameters. The spermatozoa motility at the beginning of the experiment (Time 0) found decreased rate for examined doses of Viscum album pini compared to the control, but the statistical significant reduction was observed (p
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2019.9.1.144-150
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2019
    detail.hit.zdb_id: 2641525-2
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