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  • 1
    Online Resource
    Online Resource
    Curcumin Supplementation in Semen Extender Improves Post-Thaw Quality and Antioxidant Capacity of Gir Bull Spermatozoa
    ACS Publisher ; 2022
    In:  Indian Journal of Veterinary Sciences & Biotechnology Vol. 18, No. 5 ( 2022-11-07), p. 9-13
    Details
    In: Indian Journal of Veterinary Sciences & Biotechnology, ACS Publisher, Vol. 18, No. 5 ( 2022-11-07), p. 9-13
    Abstract: This investigation was carried out on semen of three Gir bulls at Cattle Breeding Farm, Junagadh (India) for a period of 6 months. The aim was to assess the effect of different concentrations of Curcumin, viz., 0 µM (Control), 5 µM, 10 µM, and 15 µM in Andromed extender on sperm quality along with oxidative stress parameters of cryopreserved semen. Semen ejaculates (n=30, 10/bull) with 〉 70% initial motility were included in the study. Soon after initial evaluation, the semen was extended in Andromed extender @ 80 million sperm per ml and was divided into four equal aliquots. The Curcumin stock solution (10 mM) was added in these aliquots to get a final concentration of 0, 5, 10, and 15 µM and sperm motility was checked. It was soon filled and sealed in medium French straws (0.5 ml), equilibrated at 4°C for 4 h and frozen in LN2 vapour using conventional wide mouth freezer. Thawing of straws was done next day in water bath at 37°C for 30 seconds, and various sperm quality parameters including oxidative markers were assessed. The results showed that supplementation of Curcumin to freezing extender improved semen quality and antioxidant capacity. The post-thaw sperm motility, sperm viability, HOST reactive sperm and acrosomal integrity in semen extended with 15 µM Curcumin concentration (57.33±0.67, 73.20±1.05, 64.77±1.56 and 72.43±1.38 %, respectively) were significantly (p 〈 0.05) higher and sperm abnormality (18.00±0.41%) was significantly (p 〈 0.05) lower as compared to those of control extender and the 5 µM Curcumin group. Mean malondialdehyde (MDA) level in post-thaw seminal plasma did not differ significantly (p 〉 0.05) among the groups, while total antioxidants activity (TAC) with 15 µM Curcumin (476.36±16.52 μmol/L) was significantly (p 〈 0.05) higher as compared to control and 5 µM Curcumin group. It is concluded that Curcumin @ 15 µM in semen extender improves post-thaw sperm quality and antioxidant capacity of cryopreserved Gir bull semen.
    Type of Medium: Online Resource
    ISSN: 2395-1176 , 2394-0247
    URL: Article
    DOI: 10.48165/ijvsbt.18.5.02
    Language: English
    Publisher: ACS Publisher
    Publication Date: 2022
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  • 2
    Online Resource
    Online Resource
    Physico-Morphological Characteristics and Oxidative Markers of Fresh Semen of Gir Bulls
    ACS Publisher ; 2022
    In:  Indian Journal of Veterinary Sciences & Biotechnology Vol. 18, No. 4 ( 2022-09-15), p. 104-108
    Details
    In: Indian Journal of Veterinary Sciences & Biotechnology, ACS Publisher, Vol. 18, No. 4 ( 2022-09-15), p. 104-108
    Abstract: The present investigation was undertaken on four mature Gir bulls (4-6 years) maintained at University farm,  Junagadh for 4 months during hot summer season. Semen (n=6 ejaculate/bull) was collected using artificial  vagina once a week from each bull. The fresh ejaculates were evaluated for physical attributes and oxidative  stress markers in the seminal plasma. The overall mean values recorded based on 24 ejaculates for ejaculate  volume, sperm concentration, mass motility (0-5 scale), individual motility, sperm viability, sperm abnormality,  plasma membrane integrity (HOST reactive sperm), and acrosome integrity were 6.20 ± 1.27 mL, 1316.83 ±  155.07 million/ mL, 3.75 ± 0.20, 84.83 ± 0.94 %, 87.58 ± 1.07 %, 9.63 ± 0.83 %, 79.58 ± 1.01 % and 91.21 ± 0.52  %, respectively. There was a significant P 〈 0.001) difference in ejaculate volume, sperm concentration and sperm  abnormality between bulls. The levels of oxidative stress parameters, viz. lipid peroxidation and glutathione S-transferase (GST) activity in the seminal plasma were 5.51 ± 0.26 μmol/mL and 16.30 ± 2.14 nmol/ mL/min, respectively. Individual motility, viability, HOST reactive spermatozoa, and acrosomal integrity were positively correlated, suggesting that any one or two attributeslike motility or HOS test can predict the semen quality. The  semen quality generally was in normal physiological range including oxidative markers even during heat stress. 
    Type of Medium: Online Resource
    ISSN: 2395-1176 , 2394-0247
    URL: Article
    DOI: 10.48165/ijvsbt.18.4.21
    Language: Unknown
    Publisher: ACS Publisher
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Caffeine Supplementation in Semen Extender Protects Buffalo Spermatozoa from Cryodamage
    ACS Publisher ; 2022
    In:  Indian Journal of Veterinary Sciences & Biotechnology Vol. 18, No. 4 ( 2022-09-14), p. 1-5
    Details
    In: Indian Journal of Veterinary Sciences & Biotechnology, ACS Publisher, Vol. 18, No. 4 ( 2022-09-14), p. 1-5
    Abstract: Caffeine is widely known for its phosphodiesterase enzymes inhibiting, and its powerful reactive oxygen species scavenging property. This study aimed to evaluate the effect of caffeine supplementation @ 0 mM, 1 mM, 3 mM and 5 mM in AndroMed® extender on cryopreservation of Jaffarabadi buffalo semen. Twenty-four semen ejaculates (6/bull) with 〉 70% initial sperm motility were obtained from four mature Jaffarabadi bulls using AV. The ejaculates were split-diluted in an egg-yolk-free AndroMed® extender supplemented with different concentrations of caffeine and were cryopreserved using a standard protocol. The semen samples were evaluated for sperm quality parameters as well as oxidative stress parameters, viz., lipid peroxidation, and Glutathione-S-transferase (GST) enzyme activity in seminal plasma at pre-freeze (after equilibration) and post-thaw stage. The levels of caffeine had a significant effect on all these parameters, except sperm abnormalities, at both pre-freeze and post-thaw stages. Supplementation of caffeine in semen extender at 1 mM and 3 mM concentration showed a significant (p 〈 0.05) increase in post-thawed sperm motility (62.04 ± 0.84, 61.25 ± 1.01%), viability (64.21 ± 0.88, 64.25 ± 0.84%), acrosome integrity (58.08 ± 1.08, 57.30 ± 0.93%) and plasma membrane integrity (52.75 ± 0.89, 52.71 ± 0.74%) and a significant (p 〈 0.05) decrease in oxidative damage as evident by lower lipid peroxidation (MDA 7.62 ± 0.41, 7.80 ± 0.70 μM) and GST enzyme activity (31.15 ± 1.36, 29.54 ± 0.54 nmol CDNB/mL/min) as compared to control and 5 mM caffeine. The study concludes that the post-thaw quality of frozen semen of Jaffarabadi buffalo bulls improves significantly with decreased oxidative stress if the AndroMed® extender is supplemented with 1 and 3 mM concentration of caffeine over control.
    Type of Medium: Online Resource
    ISSN: 2395-1176 , 2394-0247
    URL: Article
    DOI: 10.48165/ijvsbt.18.4.01
    Language: Unknown
    Publisher: ACS Publisher
    Publication Date: 2022
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  • 4
    Online Resource
    Online Resource
    Seasonal influence on expression of heat shock proteins (HSP70 and HSP90) vis-à-vis functional competence of Gir bull semen
    Informa UK Limited ; 2023
    In:  Animal Biotechnology
    Details
    In: Animal Biotechnology, Informa UK Limited
    Type of Medium: Online Resource
    ISSN: 1049-5398 , 1532-2378
    URL: Article
    DOI: 10.1080/10495398.2023.2218453
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2043243-4
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Enriched Catalytic Activity of TiO2 Nanoparticles Supported by Activated Carbon for Noxious Pollutant Elimination
    MDPI AG ; 2021
    In:  Nanomaterials Vol. 11, No. 11 ( 2021-10-22), p. 2808-
    Details
    In: Nanomaterials, MDPI AG, Vol. 11, No. 11 ( 2021-10-22), p. 2808-
    Abstract: Cleaning wastewater has become one of the most serious issues for a number of scientists and researchers in recent years, as water is the most basic need for the daily life of humans. There has been a focus on the removal of noxious pollutants from wastewater effluents by using nanocatalysts owing to their unique physicochemical actions and stability. Herein we manufactured TiO2 nanoparticles supported by activated carbon (AC-TiO2) using a cost-effective sonochemical method. The band structures of the AC-TiO2 and TiO2 were modified from 3.2 to 3.1 eV, thus increasing the catalytic activity. The structural, optical and anatase crystal phase properties, with morphological confirmation, were studied by applying UV-DRS, PL, FESEM, XRD, along with HRTEM, respectively. The specific surface area, calculated by BET analysis, was found to be ~241 m2/gm and ~46 m2/gm for AC-TiO2 and TiO2. The degradation efficiency of the as-prepared nanocatalysts against the very toxic but rarely studied organic textile dye pollutant RO 84 was investigated and 97% efficiency were found for the AC-TiO2 as compared to pure TiO2, which is a highly appreciated finding in the catalytic dye degradation application domain. Such surface-modified nanocatalysts could be further implemented for the treatment of wastewaters/waste effluents released from chemical industries, laboratories and other sources.
    Type of Medium: Online Resource
    ISSN: 2079-4991
    URL: Article
    DOI: 10.3390/nano11112808
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2662255-5
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  • 6
    Online Resource
    Online Resource
    Experimental and Computational Approaches for the Structural Study of Novel Ca-Rich Zeolites from Incense Stick Ash and Their Application for Wastewater Treatment
    Hindawi Limited ; 2021
    In:  Adsorption Science & Technology Vol. 2021 ( 2021-11-20), p. 1-12
    Details
    In: Adsorption Science & Technology, Hindawi Limited, Vol. 2021 ( 2021-11-20), p. 1-12
    Abstract: At present, chemical Si/Al sources are mainly used as precursor materials for the manufacturing of zeolites. Such precursor materials are quite expensive for commercial synthesis. Here, we have reported the synthesis of Ca-based zeolite from incense stick ash waste by the alkali-treatment method for the first time. Incense stick ash (ISA) was used as a precursor material for the synthesis of low Si zeolites by the alkali-treatment method. The as-synthesized zeolites were characterized by various instruments like particle size analyzer (PSA), Fourier transform infrared (FTIR), X-ray diffraction (XRD), field emission scanning electron microscope (FESEM), electron diffraction spectroscopy (EDS), transmission electron microscopy (TEM), and X-ray fluorescence (XRF). FTIR and XRD helped in the identification of the microstructure and crystalline nature of the zeolites and also confirmed the synthesis of Ca-based zeolite with two thetas at 25.7°. The microscopic analysis by FESEM and TEM exhibited that the size of synthesized Ca-rich zeolites varies from 200 to 700 nm and they are aggregated and cuboidal in shape. Additionally, structural, electronic, and density of states’ characteristics of gismondine (Ca2Al4Si4O16·9H2O) structures were evaluated by computational simulations (first principle, density functional theorem). The structural optimization of structures was carried out in the first stage under the lowest condition of total energy and forces acting on atoms for the lattice constant, as well as the available experimental and theoretical findings. The present research approach predicted the transformation of ISA waste into a value-added mineral, i.e., zeolite, which was further used for the removal of both heavy metals and alkali metals from fly ash-based wastewater using inductively coupled plasma-optical emission spectroscopy (ICP-OES).
    Type of Medium: Online Resource
    ISSN: 2048-4038 , 0263-6174
    URL: Article
    DOI: 10.1155/2021/6066906
    RVK:
    VA 1500
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2017917-0
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