In:
Acta Crystallographica Section F Structural Biology and Crystallization Communications, International Union of Crystallography (IUCr), Vol. 68, No. 11 ( 2012-11-01), p. 1367-1370
Abstract:
Proliferating cell nuclear antigen (PCNA), a member of the sliding clamp family of proteins, interacts specifically with DNA replication and repair proteins through a small peptide motif called the PCNA-interacting protein or PIP box. PCNA is recognized as one of the key proteins involved in DNA metabolism. In the present study, the recombinant PCNA from Litopenaeus vannamei ( Lv PCNA) was heterologously overexpressed and purified using metal ion-affinity chromatography. Crystals suitable for diffraction grew overnight using the hanging-drop vapour-diffusion method. Lv PCNA crystals belong to space group C 2 with unit-cell parameters a = 144.6, b = 83.4, c = 74.3 Å, β = 117.6°. One data set was processed to 3 Å resolution, with an overall R meas of 0.09 and a completeness of 93.3%. Initial phases were obtained by molecular replacement using a homology model of Lv PCNA as the search model. Refinement and structural analysis are underway. This report is the first successful crystallographic analysis of a marine crustacean decapod shrimp ( L. vannamei ) proliferating cell nuclear antigen.
Type of Medium:
Online Resource
ISSN:
1744-3091
DOI:
10.1107/S1744309112040444
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2012
detail.hit.zdb_id:
2175956-X
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