In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 9 ( 2023-9-25), p. e3002292-
Abstract:
Sulfate-coupled anaerobic oxidation of methane (AOM) is performed by multicellular consortia of anaerobic methanotrophic archaea (ANME) in obligate syntrophic partnership with sulfate-reducing bacteria (SRB). Diverse ANME and SRB clades co-associate but the physiological basis for their adaptation and diversification is not well understood. In this work, we used comparative metagenomics and phylogenetics to investigate the metabolic adaptation among the 4 main syntrophic SRB clades (HotSeep-1, Seep-SRB2, Seep-SRB1a, and Seep-SRB1g) and identified features associated with their syntrophic lifestyle that distinguish them from their non-syntrophic evolutionary neighbors in the phylum Desulfobacterota. We show that the protein complexes involved in direct interspecies electron transfer (DIET) from ANME to the SRB outer membrane are conserved between the syntrophic lineages. In contrast, the proteins involved in electron transfer within the SRB inner membrane differ between clades, indicative of convergent evolution in the adaptation to a syntrophic lifestyle. Our analysis suggests that in most cases, this adaptation likely occurred after the acquisition of the DIET complexes in an ancestral clade and involve horizontal gene transfers within pathways for electron transfer (CbcBA) and biofilm formation (Pel). We also provide evidence for unique adaptations within syntrophic SRB clades, which vary depending on the archaeal partner. Among the most widespread syntrophic SRB, Seep-SRB1a, subclades that specifically partner ANME-2a are missing the cobalamin synthesis pathway, suggestive of nutritional dependency on its partner, while closely related Seep-SRB1a partners of ANME-2c lack nutritional auxotrophies. Our work provides insight into the features associated with DIET-based syntrophy and the adaptation of SRB towards it.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002292
DOI:
10.1371/journal.pbio.3002292.g001
DOI:
10.1371/journal.pbio.3002292.g002
DOI:
10.1371/journal.pbio.3002292.g003
DOI:
10.1371/journal.pbio.3002292.g004
DOI:
10.1371/journal.pbio.3002292.g005
DOI:
10.1371/journal.pbio.3002292.g006
DOI:
10.1371/journal.pbio.3002292.g007
DOI:
10.1371/journal.pbio.3002292.t001
DOI:
10.1371/journal.pbio.3002292.s001
DOI:
10.1371/journal.pbio.3002292.s002
DOI:
10.1371/journal.pbio.3002292.s003
DOI:
10.1371/journal.pbio.3002292.s004
DOI:
10.1371/journal.pbio.3002292.s005
DOI:
10.1371/journal.pbio.3002292.s006
DOI:
10.1371/journal.pbio.3002292.s007
DOI:
10.1371/journal.pbio.3002292.s008
DOI:
10.1371/journal.pbio.3002292.s009
DOI:
10.1371/journal.pbio.3002292.s010
DOI:
10.1371/journal.pbio.3002292.s011
DOI:
10.1371/journal.pbio.3002292.s012
DOI:
10.1371/journal.pbio.3002292.s013
DOI:
10.1371/journal.pbio.3002292.s014
DOI:
10.1371/journal.pbio.3002292.s015
DOI:
10.1371/journal.pbio.3002292.s016
DOI:
10.1371/journal.pbio.3002292.s017
DOI:
10.1371/journal.pbio.3002292.s018
DOI:
10.1371/journal.pbio.3002292.s019
DOI:
10.1371/journal.pbio.3002292.s020
DOI:
10.1371/journal.pbio.3002292.s021
DOI:
10.1371/journal.pbio.3002292.s022
DOI:
10.1371/journal.pbio.3002292.s023
DOI:
10.1371/journal.pbio.3002292.s024
DOI:
10.1371/journal.pbio.3002292.s025
DOI:
10.1371/journal.pbio.3002292.s026
DOI:
10.1371/journal.pbio.3002292.s027
DOI:
10.1371/journal.pbio.3002292.s028
DOI:
10.1371/journal.pbio.3002292.s029
DOI:
10.1371/journal.pbio.3002292.s030
DOI:
10.1371/journal.pbio.3002292.s031
DOI:
10.1371/journal.pbio.3002292.s032
DOI:
10.1371/journal.pbio.3002292.s033
DOI:
10.1371/journal.pbio.3002292.s034
DOI:
10.1371/journal.pbio.3002292.s035
DOI:
10.1371/journal.pbio.3002292.s036
DOI:
10.1371/journal.pbio.3002292.s037
DOI:
10.1371/journal.pbio.3002292.s038
DOI:
10.1371/journal.pbio.3002292.r001
DOI:
10.1371/journal.pbio.3002292.r002
DOI:
10.1371/journal.pbio.3002292.r003
DOI:
10.1371/journal.pbio.3002292.r004
DOI:
10.1371/journal.pbio.3002292.r005
DOI:
10.1371/journal.pbio.3002292.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
Bookmarklink