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  • 1
    Online Resource
    Online Resource
    REVIEWS
    Informa UK Limited ; 1991
    In:  The Mariner's Mirror Vol. 77, No. 1 ( 1991-01), p. 82-96
    Details
    In: The Mariner's Mirror, Informa UK Limited, Vol. 77, No. 1 ( 1991-01), p. 82-96
    Type of Medium: Online Resource
    ISSN: 0025-3359 , 2049-680X
    URL: Article
    DOI: 10.1080/00253359.1991.10656339
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 1991
    detail.hit.zdb_id: 2710690-1
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  • 2
    Online Resource
    Online Resource
    Improvement of maternal vitamin D status with 25-hydroxycholecalciferol positively impacts porcine fetal skeletal muscle development and myoblast activity1,2
    Oxford University Press (OUP) ; 2013
    In:  Journal of Animal Science Vol. 91, No. 9 ( 2013-09-01), p. 4116-4122
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 91, No. 9 ( 2013-09-01), p. 4116-4122
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.2527/jas.2013-6565
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2013
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Feeding 25-hydroxycholecalciferol improves gilt reproductive performance and fetal vitamin D status1
    Oxford University Press (OUP) ; 2012
    In:  Journal of Animal Science Vol. 90, No. 11 ( 2012-11-01), p. 3783-3788
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 90, No. 11 ( 2012-11-01), p. 3783-3788
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.2527/jas.2011-5023
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2012
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Efficacy and safety of a novel source of dietary 25-hydroxycholecalciferol in growing pigs
    Oxford University Press (OUP) ; 2022
    In:  Journal of Animal Science Vol. 100, No. 9 ( 2022-09-01)
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. 9 ( 2022-09-01)
    Abstract: A randomized complete block design experiment was conducted to determine the safety and efficacy of supplementation of increasing concentrations of a novel, bacterial fermentation-derived vitamin D source on growth performance and tissue deposition of 25-hydroxycholecalciferol (25OHD3) in growing swine. Dietary treatments were as follows: commercial control with vitamin D3 (CON) at NRC recommended concentrations and three diets composed of CON + increasing inclusions (25, 50, and 250 µg/kg equivalent) of 25OHD3 from a novel source (CON + 25; CON + 50; and CON + 250, respectively). Pigs (n = 144) were assigned to 24 pens which were allotted to one of the four dietary treatments and fed for 42 d. Blood samples were collected for 25OHD3 concentration determination and individual body weights (BW) were measured on experimental day 0, 39, and 63. On day 42, tissues from 48 pigs (12 pigs per dietary treatment) were analyzed for 25OHD3 concentration. No differences were observed in growth performance. Day 39 serum 25OHD3 concentrations were greatest in CON + 250-fed pigs and linearly decreased as dietary 25OHD3 inclusion decreased (P  & lt; 0.0001). On day 42, tissue 25OHD3 concentrations increased linearly as 25OHD3 increased in the diet (P  & lt; 0.0001). On day 63, 21 d after dietary 25OHD3 withdrawal, serum 25OHD3 concentrations of all 25OHD3-fed pigs decreased to that of or within 2.76 ± 0.89 ng/mL of CON-fed pigs which demonstrates that feeding 250 µg/kg 25OHD3 is well tolerated by growing pigs and will clear the body within 21 d.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skac260
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    383 Scientific Training Programs for Undergraduate and Graduate Students: Applied Animal and Food Science
    Oxford University Press (OUP) ; 2022
    In:  Journal of Animal Science Vol. 100, No. Supplement_3 ( 2022-09-22), p. 187-188
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. Supplement_3 ( 2022-09-22), p. 187-188
    Abstract: Training in animal sciences can be difficult to cover in a comprehensive manner. So many different areas are necessary to prepare students for the multiple employment opportunities afforded them upon graduation. This is an approach where students are trained in both experiential animal husbandry techniques as well as some areas of fundamental sciences. A farm to fork approach is being taken to demonstrate the varied areas of the industry and assist students in finding their preferred discipline. Initially, students are taught the basics of nutrition and animal food manufacturing to expose them to this aspect of the allied industry. Practical interactive methodology for raising both poultry and swine are developed through the daily welfare checks conducted by students twice daily at our research facilities. Through these activities students are taught to properly observe and conduct practices of feeding, welfare needs of animals, and health observations. Husbandry techniques are taught and demonstrated and then students are allowed to develop their competency through both participatory and observational methodologies during animal management observations with experienced students and advisor input including questions and answers. Exposure of animal performance objectives and management are delivered to better prepare students for the live animal aspect of the production industry. Additionally, students are involved in actual protein conversion and production of food for human nutritional needs. Students may not fully comprehend opportunities and applications of the protein conversion side of industry. Practical experiential learning is achieved through first-hand experience in the aspects of the protein production industry. Using food science technologies and interactive examples, students also learn about production of pet foods derived from low value co-products from both mammalian and poultry protein conversion. Exposure to both initial meat production and further processing unlocks opportunities for students and their employment success indicates the success of this approach.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skac247.345
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    384 Scientific Training Programs for Undergraduate and Graduate Students: Exposure to Fundamental Science
    Oxford University Press (OUP) ; 2022
    In:  Journal of Animal Science Vol. 100, No. Supplement_3 ( 2022-09-22), p. 188-188
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. Supplement_3 ( 2022-09-22), p. 188-188
    Abstract: Training students in animal science can be difficult to achieve in a comprehensive manner. It is a multidisciplinary field where many different areas are necessary to successfully prepare students at multiple levels for the multiple employment opportunities they can choose from as they near graduation and enter the workforce. The approach we have taken with our teaching program is one where students are trained in both experiential animal husbandry techniques as well as areas of fundamental sciences such as cellular and molecular biology. A farm to fork systems approach is being taken to demonstrate the varied areas of study available and aid students in finding their niche. Students first spend time acquiring practical and hands-on experience in animal husbandry, animal food manufacturing, and meat processing and food science research. Most of the projects that students participate in while acquiring animal husbandry skills involve several collections of various types of samples over the animal rearing period for cell isolation and in vitro culture, proteomics, cell-labeling technologies, cryohistology, immunofluorescence staining, and digital microscopy. Our research program focuses primarily on the influence of management and nutrition on the development of the local intestinal immune system, development and growth of the intestine and skeletal muscle and how those interact to impact the overall growth and health of livestock. Exposing students to some of the different types of analyses conducted in research involving fundamental science allows them to gain skills required to determine the mechanisms behind the more applied research results they obtain. Integrating applied and fundamental science in a research setting helps prepare students for several different avenues of employment upon completion of the program. The overall success of this training program has been demonstrated over the last 5 years based on student and employer feedback.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skac247.346
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Data_Sheet_1.PDF
    Frontiers Media SA ; 2022
    In:  Frontiers in Veterinary Science Vol. 9 ( 2022-7-11)
    Details
    In: Frontiers in Veterinary Science, Frontiers Media SA, Vol. 9 ( 2022-7-11)
    Abstract: Vitamin D signaling is important for intestinal homeostasis. An increase in vitamin D receptors in immune cells can modulate cell phenotype and cytokine secretion. Cytokines regulate both pro- (interleukin 17; IL-17) and anti-inflammatory (IL-10) responses triggered by external stimuli. Inflammation in intestinal tissues can disrupt the structure and the remodeling of epithelial tight junction complexes, thus, compromising the protective barrier. The objective of the study was to determine the impact of dietary supplementation with 25-hydroxycholecalciferol ( 25 OHD 3 ), a hydroxylated metabolite of vitamin D, on intestinal cytokine abundance and epithelial barrier integrity over time in broilers. A randomized complete block design experiment was conducted to evaluate the effect of dietary 25 OHD 3 inclusion on relative protein expression of the cytokines, IL-17 and IL-10, and tight junction proteins, Zona Occludens 1 (ZO-1), and Claudin-1 (CLD-1), in broiler chicken duodenum and ileum from 3 to 21 days post-hatch. On day 0, male chicks ( n = 168) were randomly assigned to raised floor pens. Experimental corn–soybean meal-based treatments were as follows: (1) a common starter diet containing 5,000 IU of D 3 per kg of feed (VITD 3 ) and (2) a common starter diet containing 2,240 IU of D 3 + 2,760 IU of 25 OHD 3 per kg of feed ( 25 OHD 3 ) fed from days 0 to 21. On days 3, 6, 9, 12, 15, 18, and 21, 12 birds per treatment were euthanized to collect tissue samples for quantitative, multiplex, and fluorescent Western blot analysis. Target proteins were quantified using Image Quant TL 8.1 and expressed relative to total protein. Feeding 25 OHD 3 post-hatch decreased ileal IL-10 (anti-inflammatory) protein expression in 21-day-old broilers compared with VITD 3 only ( P = 0.0190). Broilers fed only VITD 3 post-hatch had greater IL-17 (pro-inflammatory) protein expression in the ileum at 18 and 21 days-of-age ( P = 0.0412) than those that fed 25 OHD 3 . Dietary inclusion of 25 OHD 3 lowered the abundance of key inflammatory cytokines in the ileum of young broilers.
    Type of Medium: Online Resource
    ISSN: 2297-1769
    URL: Article
    URL: Article
    DOI: 10.3389/fvets.2022.947276
    DOI: 10.3389/fvets.2022.947276.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2834243-4
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  • 8
    Online Resource
    Online Resource
    Combining Maternal and Post-Hatch Dietary 25-Hydroxycholecalciferol Supplementation on Broiler Chicken Growth Performance and Carcass Characteristics
    MDPI AG ; 2022
    In:  Poultry Vol. 1, No. 2 ( 2022-05-24), p. 111-124
    Details
    In: Poultry, MDPI AG, Vol. 1, No. 2 ( 2022-05-24), p. 111-124
    Abstract: Dietary inclusion of the vitamin D3 (D3) metabolite, 25-hydroxycholecalciferol (25OHD3), was demonstrated to improve broiler growth performance and breast meat yield. To assess the effect of combined maternal (MDIET) and post-hatch (PDIET) dietary 25OHD3 inclusion on broiler growth performance and carcass characteristics, a randomized complete block design experiment with a 2 × 2 factorial treatment structure was conducted. From 25 to 38 weeks of age, broiler breeder hens were provided with 1 of 2 MDIET formulated to contain: 5000 IU D3 (MCTL), or 2240 IU of D3 + 2760 IU of 25OHD3 per kg of feed (M25OHD3). Their chick offspring (n = 448; 224 per MDIET) hatched from eggs collected from 37 to 38 weeks of age were reared in 16 replicate pens with 7 birds per pen and fed 1 of 2 PDIET in 3 phases up to day 40 formulated to contain: 5000 IU of D3 per kg of feed (PCTL), or 2240 IU of D3 + 2760 IU of 25OHD3 per kg of feed (P25OHD3). No additive or synergistic effects of combining 25OHD3 inclusion in MDIET and PDIET were observed. Broilers from 25OHD3-fed hens (M25OHD3) were heavier on day 40 than those from hens fed only D3 (MCTL; 2.911 vs. 2.834 kg; p = 0.040). Tender weight (123 vs. 117 g) and yield (5.63 vs. 5.44%) were greater in the M25OHD3 broilers than the MCTL broilers (p = 0.006). Broilers fed 25OHD3 (P25OHD3) tended to have heavier breasts (637 vs. 615 g; p = 0.050), bone-in wings (215 vs. 210 g; p = 0.070), and boneless thighs (279 vs. 270 g; p = 0.078) compared with those fed only D3 (PCTL). Neither MDIET nor PDIET altered the severity of Wooden Breast and White Striping (p ≥ 0.106). Overall, including 25OHD3 in either the maternal or broiler diet increased broiler meat yield.
    Type of Medium: Online Resource
    ISSN: 2674-1164
    URL: Article
    DOI: 10.3390/poultry1020010
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 3136491-3
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  • 9
    Online Resource
    Online Resource
    263 Efficacy and Safety of a Novel Source of 25-Hydroxycholecalciferol in Performance and Tissue Deposition in Growing Pigs
    Oxford University Press (OUP) ; 2022
    In:  Journal of Animal Science Vol. 100, No. Supplement_3 ( 2022-09-22), p. 112-113
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. Supplement_3 ( 2022-09-22), p. 112-113
    Abstract: This study was conducted to determine the efficacy and safety of a dietary vitamin D source on growth performance and tissue deposition of 25-hydroxycholecalciferol (25OHD3) in growing swine. Dietary treatments were a control with vitamin D3 at NRC recommended concentrations (C) and 3 diets composed of C + increasing inclusions (25, 50, and 250 µg/kg BioD, Huvepharma) of 25OHD3 (C+25; C+50; and C+250 respectively). Pigs (n = 144) were assigned to 24 pens which were allotted to 1 of 4 dietary treatments and fed for 42 d. On d 0, 39, and 63, serum was collected for 25OHD3 concentration determination and individual BW were measured. At d 42, tissues from 48 pigs (12 pigs per treatment) were analyzed for 25OHD3 concentration, blood chemistry and hematology (BCH) analysis was conducted, and 1 femur from each pig was collected for dual-energy X-ray absorptiometry scanning. Data were analyzed with SAS PROC GLIMMIX and means separated at P ≤ 0.05. No differences among treatments were observed for growth performance (P ≥ 0.2239). Serum and tissue 25OHD3 concentrations increased linearly as dietary 25OHD3 inclusion increased (P & lt; 0.0001). Following a 21-d withdrawal period, 25OHD3 serum concentrations decreased to that of or within 2.76 ± 0.89 ng/mL of C-fed pigs (P & gt; 0.0005). All BCH variables measured were similar among treatments (P = 0.0967). At day 42, femurs from C+50 and C+250-fed pigs had the greatest bone mineral content (BMC) and density (BMD; P & lt; 0.0326). Concentrations of 25OHD3 in serum and tissue increased and BMC and BMD were improved as dietary vitamin D supplementation increased, but pig growth performance was unaltered. Absence of gross abnormalities and changes in BCH as well return of circulating 25OHD3 concentrations to that of C-fed pigs following withdrawal indicate this source can safely improve vitamin D status in growing pigs.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skac247.217
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation
    MDPI AG ; 2022
    In:  Animals Vol. 12, No. 11 ( 2022-05-31), p. 1425-
    Details
    In: Animals, MDPI AG, Vol. 12, No. 11 ( 2022-05-31), p. 1425-
    Abstract: The objective of this experiment was to access primary satellite cell (SC) proliferation and differentiation when cultured in different combinations of basal media and sera due to little consistency being published on the optimal culture media for primary broiler chicken satellite cells. Cells were cultured in one of three different basal media: McCoy’s 5A, high glucose Dulbecco’s Modified Eagle’s medium (DMEM), and low glucose DMEM. Media were supplemented with 15% chicken serum (CS) or a combination of 5% horse serum (HS) + 10% CS during proliferation while 3% HS or 3% CS were added to the media during differentiation. Cultures were immunofluorescence stained for myogenic regulatory factors (MRF) at 48, 72, and 96 h post-plating for proliferation (Pax7, MyoD, and Myf-5) and 96 h post-proliferation during differentiation (Pax7 and MyoD), including MF20 to assess fusion. Cells cultured in Dulbecco’s Modified Eagle’s medium tended to have higher proportions of myogenic cells expressing MRF during proliferation and promoted fusion into myotubes compared with McCoy’s 5A during differentiation. Culturing primary SC in low glucose media, glucose concentrations similar to circulating glucose concentrations in broilers, HSCS during proliferation and CS during differentiation, appears to be optimal for promoting broiler chicken satellite cell proliferation and differentiation.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani12111425
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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