In:
Annals of the New York Academy of Sciences, Wiley, Vol. 987, No. 1 ( 2003-04), p. 166-172
Abstract:
A bstract : The germinal center (GC) reaction in T cell dependent antibody responses is crucial for the generation of B cell memory and plays a critical role in B cell lymphomagenesis. To gain insight into the physiology of this reaction, we identified the transcriptional changes that occur in B cells during the GC‐transit (naïve B cells → CD77 + centroblasts (CBs) → CD77 − centrocytes (CCs) → memory B cells) by DNA microarray experiments and the subsequent data analysis employing unsupervised and supervised hierarchical clustering. The naïve B cell is characterized by a nonproliferative, anti‐apoptotic phenotype and the expression of various chemokine and cytokine receptors. The transition from naïve B cells to CBs is associated with (1) the up‐regulation of genes associated with cellular proliferation, DNA‐repair, and chromatin remodeling; (2) the acquisition of a pro‐apoptotic phenotype; (3) the down‐regulation of cytokine, chemokine, and adhesion receptors expressed in the naïve cells; and (4) the expression of a distinct adhesion repertoire. The CB and the CC revealed surprisingly few gene expression differences, suggesting that the CC is heterogeneous in its cellular composition. The CB/CC to memory B cell transition shows a general reversion to the profile characteristic for the naïve B cells, with the exception of the up‐regulation of several surface receptors, including CD27, CD80, and IL‐2Rβ, and the simultaneous expression of both anti‐ and pro‐apoptotic genes. These gene expression profiles of the normal B cell subpopulations are being used to identify the signals occurring during GC development, the cellular derivation of various types of B cell malignancies, and the genes deregulated in GC‐derived tumors.
Type of Medium:
Online Resource
ISSN:
0077-8923
,
1749-6632
DOI:
10.1111/nyas.2003.987.issue-1
DOI:
10.1111/j.1749-6632.2003.tb06045.x
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2834079-6
detail.hit.zdb_id:
211003-9
detail.hit.zdb_id:
2071584-5
SSG:
11
Bookmarklink