In:
Acta Biochimica Polonica, Polskie Towarzystwo Biochemiczne (Polish Biochemical Society), Vol. 60, No. 4 ( 1970-01-01)
Abstract:
In the diadinoxanthin cycle the epoxy group is removed from diadinoxanthin and diatoxanthin is created. This conversion takes place e.g. in diatoms with the involvement of the enzyme diadinoxanthin de-epoxidase. In one of the diatom species, Phaeodactylum tricornutum (CCAP 1055/1 strain with genome sequenced) three de-epoxidase genes (PtVDE, PtVDL1, PtVDL2) have been identified, but only one of them (PtVDE) corresponds to violaxanthin de-epoxidase, an enzyme which is commonly found in higher plants. In these studies, the expression of two de-epoxidase genes of another Phaeodactylum tricornutum strain (UTEX 646), which is commonly used in diatom studies, were obtained in Origami b and BL21 E. coli strains. The molecular masses of the mature proteins are about 49 kDa and 60 kDa, respectively, for VDE and VDL2. Both enzymes are active with violaxanthin as a substrate.
Type of Medium:
Online Resource
ISSN:
1734-154X
,
0001-527X
DOI:
10.18388/abp.2013_2072
Language:
Unknown
Publisher:
Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)
Publication Date:
1970
detail.hit.zdb_id:
2086022-5
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