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PCR Detection of Epstein-Barr Virus (EBV) DNA in Patients with Head and Neck Squamous Cell Carcinoma, in Patients with Chronic Tonsillitis, and in Healthy Individuals

Strzelczyk, Joanna Katarzyna ; Świętek, Agata ; Biernacki, Krzysztof ; [et al.]

Hindawi Limited ; 2022

In: BioMed Research International Vol. 2022 ( 2022-8-8), p. 1-8

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In: BioMed Research International, Hindawi Limited, Vol. 2022 ( 2022-8-8), p. 1-8

Abstract: Epstein-Barr virus (EBV) is a common virus worldwide that is an etiologic agent in the development of many diseases, including cancer. Recent reports have shown the association of EBV with tumorigenesis in head and neck squamous cell carcinoma (HNSCC). Moreover, EBV has been reported to be present in tonsillar tissues, which suggests a close relationship between viral infections and tonsillar diseases, including chronic tonsillitis. The aim of the study was to analyze the prevalence of EBV DNA in 86 patients with HNSCC, in 70 patients with chronic tonsillitis, and in 144 healthy individuals (control group) and the associations between EBV infection and clinicopathological and demographic characteristics and the use of stimulants in all study groups. The objective of this study was also to analyze the prevalence of coinfection with human papillomavirus (HPV). After prior DNA isolation, EBV detection was performed using an EBV kit by real-time polymerase chain reaction. The prevalence of EBV infection in patients with HNSCC, patients with chronic tonsillitis, and the control group was 47.7%, 60%, and 24.3%, respectively. Compared to controls, a significantly higher prevalence of EBV in patients with chronic tonsillitis and HNSCC may suggest that EBV is a potential risk factor. No association was found between EBV infection and demographic or clinical data. Further studies are warranted due to inconclusive reports that were mainly related to geographic distribution, sample type, and detection technique. Considering the prevalence of the virus and the risk of serious diseases, attention should be paid to screening diagnosis and prevention of the infection.

Type of Medium: Online Resource

ISSN: 2314-6141 , 2314-6133

URL: Article

DOI: 10.1155/2022/8506242

Language: English

Publisher: Hindawi Limited

Publication Date: 2022

detail.hit.zdb_id: 2698540-8

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2

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Low Prevalence of HSV-1 and Helicobacter pylori in HNSCC and Chronic Tonsillitis Patients Compared to Healthy Individuals

Strzelczyk, Joanna Katarzyna ; Świętek, Agata ; Hudy, Dorota ; [et al.]

MDPI AG ; 2023

In: Diagnostics Vol. 13, No. 10 ( 2023-05-19), p. 1798-

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In: Diagnostics, MDPI AG, Vol. 13, No. 10 ( 2023-05-19), p. 1798-

Abstract: Recent studies identified viral and bacterial factors, including HSV-1 and H. pylori, as possible factors associated with diseases such as chronic tonsillitis and cancers, including head and neck squamous cell carcinoma (HNSCC). We assessed the prevalence of HSV-1/2 and H. pylori in patients with HNSCC, chronic tonsillitis, and healthy individuals using PCR after DNA isolation. Associations were sought between the presence of HSV-1, H. pylori, and clinicopathological and demographic characteristics and stimulant use. HSV-1 and H. pylori were most frequently identified in controls (HSV-1: 12.5% and H. pylori: 6.3%). There were 7 (7.8%) and 8 (8.6%) patients with positive HSV-1 in HNSCC and chronic tonsillitis patients, respectively, while the prevalence of H. pylori was 0/90 (0%) and 3/93 (3.2%), respectively. More cases of HSV-1 were observed in older individuals in the control group. All positive HSV-1 cases in the HNSCC group were associated with advanced tumor stage (T3/T4). The prevalence of HSV-1 and H. pylori was highest in the controls compared to HNSCC and chronic tonsillitis patients, which indicates that the pathogens were not risk factors. However, since all positive HSV-1 cases in the HNSCC group were observed only in patients with advanced tumor stage, we suggested a possible link between HSV-1 and tumor progression. Further follow-up of the study groups is planned.

Type of Medium: Online Resource

ISSN: 2075-4418

URL: Article

DOI: 10.3390/diagnostics13101798

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2662336-5

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The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

Strzelczyk, Joanna Katarzyna ; Biernacki, Krzysztof ; Gaździcka, Jadwiga ; [et al.]

MDPI AG ; 2021

In: Diagnostics Vol. 11, No. 12 ( 2021-11-24), p. 2180-

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In: Diagnostics, MDPI AG, Vol. 11, No. 12 ( 2021-11-24), p. 2180-

Abstract: Human papillomavirus (HPV) is a virus with the potential to infect human epithelial cells and an etiological agent of many types of cancer, including head and neck cancer. The aim of the study was to determine the prevalence of HPV infection in patients with head and neck squamous cell carcinoma (HNSCC), patients with chronic tonsillitis, and healthy individuals, and to establish high- and low-risk HPV genotypes in these groups. The objectives also comprised the delineation of the relationship between the infection with high- or low-risk HPV subtypes and clinicopathological and demographic characteristics of the study groups. This study was composed of 76 patients diagnosed with HNSCC, 71 patients with chronic tonsillitis, and 168 cases without either of these conditions (the control group). HPV detection and identification of subtypes were performed on isolated DNA using a test which allowed detection of 33 common high-risk and low-risk HPV subtypes. The prevalence of HPV infection was 42.1%, 25.4%, and 37.5% in HNSCC, chronic tonsillitis, and control groups, respectively. HPV 16 was the most prevalent genotype in all groups and the non-oncogenic HPV 43/44 was frequent in HNSCC patients. This analysis provides insight into the prevalence of oral oncogenic and non-oncogenic HPVs in patients with head and neck cancer, patients with chronic tonsillitis and healthy individuals, and leads to the conclusion that further investigations are warranted to examine a larger cohort of patients focusing on high- and low-risk HPV genotypes. Efforts should be focused on screening and prevention strategies, and therefore, it is important to introduce tools for effective detection of HPV genotypes. Furthermore, given the role of vaccines against oral HPV infection, our observations lead to the suggestion that HPV vaccination should be of considerable importance in public health strategies.

Type of Medium: Online Resource

ISSN: 2075-4418

URL: Article

DOI: 10.3390/diagnostics11122180

Language: English

Publisher: MDPI AG

Publication Date: 2021

detail.hit.zdb_id: 2662336-5

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Selected Parameters of Bone Turnover in Neuroendocrine Tumors—A Potential Clinical Use?

Strzelczyk, Janusz ; Wójcik-Giertuga, Monika ; Strzelczyk, Joanna Katarzyna ; [et al.]

MDPI AG ; 2023

In: Journal of Clinical Medicine Vol. 12, No. 14 ( 2023-07-11), p. 4608-

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In: Journal of Clinical Medicine, MDPI AG, Vol. 12, No. 14 ( 2023-07-11), p. 4608-

Abstract: Background: Currently, there are no effective markers to diagnose and monitor patients with neuroendocrine tumors (NETs). The aim of this study was to assess bone metabolism based on selected markers of bone turnover: OST, OPG, and IGFBP-3, in both the group of patients with NETs and the control group. Associations with selected sociodemographic, biochemical, and clinicopathological characteristics were examined. We also evaluated any potential associations between these markers and selected biochemical markers of NETs commonly used in clinical practice. Methods: The study group included 60 patients with GEP-NETs and BP-NETs, while the control group comprised 62 healthy individuals. The serum concentrations of OST, OPG and IGFBP-3 were assessed using ELISA. Results: OST and OPG levels were significantly higher in the study group compared to the control group. In the study group, we observed a significant correlation between OPG and the clinical stage and chromogranin A. Additionally, an association was found between OPG and histological grade, Ki-67, and metastasis in GEP-NET cases. Conclusions: Markers of bone turnover cannot be used in the routine diagnostics of neuroendocrine tumors. Nonetheless, these markers may help evaluate the skeletal system in patients with NETs. Further research is needed to determine the utility of osteocalcin (OST) and osteoprotegerin (OPG) as potential biomarkers for neuroendocrine tumors.

Type of Medium: Online Resource

ISSN: 2077-0383

URL: Article

DOI: 10.3390/jcm12144608

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2662592-1

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Assessment of the Concentration of Endogenous Factors Regulating Angiogenesis, VASH-1 and VEGF-A, in the Blood Serum of Patients with Neuroendocrine Neoplasms

Strzelczyk, Janusz ; Wójcik-Giertuga, Monika ; Cuber, Piotr ; [et al.]

Hindawi Limited ; 2022

In: BioMed Research International Vol. 2022 ( 2022-3-10), p. 1-8

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In: BioMed Research International, Hindawi Limited, Vol. 2022 ( 2022-3-10), p. 1-8

Abstract: Neuroendocrine neoplasms (NENs) constitute about 2% of all malignant neoplasms, and the angiogenesis process in these tumors is still of a great interest. Vasohibin-1 (VASH-1) is an angiogenesis inhibitor, while vascular endothelial growth factor A (VEGF-A) is one of the main factors promoting vascular formation. The subject of this study was to assess serum concentration of these factors in patients with diagnosed NEN and in control group. Methods. The study group consisted of 120 patients with diagnosed NENs, while the control group consisted of 69 healthy volunteers. The concentrations of VASH-1 and VEGF-A in serum were tested using the ELISA. We also analyzed the association of the concentration of these factors with demographic data (e.g., age and gender), body mass index (BMI), primary tumor location, histological grade, metastasis, clinical staging, selected biochemical parameters and markers of NENs, and information on smoking habits. Results. The mean concentration of VASH-1 was 218.8 ± 359.8 pg/ml in the study group and 973.1 ± 1239.4 pg/ml in the control group, that difference was statistically significant ( p 〈 0.05 ). In the NEN group, the highest concentration of VASH-1 was in patients with pancreatic NENs in relation to NENs with different location of the primary tumor ( p 〈 0.05 ). Negative correlation was found between the concentration of VASH-1 and serotonin ( r S = − 0.19 , p 〈 0.05 ). No statistically significant differences were observed for VEGF-A ( p = 0.658 ). Conclusions. Patients with NENs showed lower serum level of VASH-1 in comparison to healthy volunteers. The highest level of VASH-1 was observed in tumors localized in pancreas. This might reflect the relevant function of VASH-1 in NENs and requires further evaluation to further knowledge of angiogenesis in NENs. Furthermore, the serum concentration of VEGF-A showed no statistical differences and probably does not have diagnostic value in this group of patients.

Type of Medium: Online Resource

ISSN: 2314-6141 , 2314-6133

URL: Article

DOI: 10.1155/2022/9084393

Language: English

Publisher: Hindawi Limited

Publication Date: 2022

detail.hit.zdb_id: 2698540-8

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Sequencing Analysis of MUC6 and MUC16 Gene Fragments in Patients with Oropharyngeal Squamous Cell Carcinoma Reveals Novel Mutations: A Preliminary Study

Gaździcka, Jadwiga ; Biernacki, Krzysztof ; Salatino, Silvia ; [et al.]

MDPI AG ; 2023

In: Current Issues in Molecular Biology Vol. 45, No. 7 ( 2023-07-04), p. 5645-5661

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In: Current Issues in Molecular Biology, MDPI AG, Vol. 45, No. 7 ( 2023-07-04), p. 5645-5661

Abstract: The growing incidence of oropharyngeal squamous cell carcinoma (OPSCC) calls for better understanding of the mutational landscape of such cases. Mucins (MUCs) are multifunctional glycoproteins expressed by the epithelial cells and may be associated with the epithelial tumour invasion and progression. The present study aimed at the analysis of the sequence of selected MUC6 and MUC16 gene fragments in the tumour, as well as the margin, samples obtained from 18 OPSCC patients. Possible associations between the detected mutations and the clinicopathological and demographic characteristics of the study group were analysed. Sanger sequencing and bioinformatic data analysis of the selected MUC6 and MUC16 cDNA fragments were performed. Our study found 13 and 3 mutations in MUC6 and MUC16, respectively. In particular, one novelty variant found that the MUC6 gene (chr11:1018257 A 〉 T) was the most frequent across our cohort, in both the tumour and the margin samples, and was then classified as a high impact, stop-gain mutation. The current study found novel mutations in MUC6 and MUC16 providing new insight into the genetic alternation in mucin genes among the OPSCC patients. Further studies, including larger cohorts, are recommended to recognise the pattern in which the mutations affect oropharyngeal carcinogenesis.

Type of Medium: Online Resource

ISSN: 1467-3045

URL: Article

DOI: 10.3390/cimb45070356

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2090836-2

SSG: 12

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Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma

Gołąbek, Karolina ; Rączka, Grzegorz ; Gaździcka, Jadwiga ; [et al.]

MDPI AG ; 2022

In: Biomedicines Vol. 10, No. 12 ( 2022-11-23), p. 3011-

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In: Biomedicines, MDPI AG, Vol. 10, No. 12 ( 2022-11-23), p. 3011-

Abstract: Background: Oral squamous cell carcinoma (OSCC) is one of the most commonly detected neoplasms worldwide. Not all mechanisms associated with cell cycle disturbances are known in OSCC. Examples of genes involved in the control of the cell cycle are CDKN2A, MDM2, E2F2 and LTF. The aim of this study was to examine the possible association between CDKN2A, MDM2, E2F2 and LTF mRNA expression and influence on clinical variables. Methods: The study group consisted of 88 Polish patients. The gene expression levels were assessed by quantitative reverse transcription PCR. Results: We found no statistically significant differences in the expression level of CDKN2A, MDM2, E2F2 and LTF genes in tumour samples compared to margin samples. No association was found between the gene expression levels and clinical parameters, except E2F2. The patients with G2 tumours had a significantly higher gene expression level of E2F2 than patients with low-grade G1 tumours. Conclusions: We have not demonstrated that a change in expression profiles of genes has a significant impact on the pathogenesis of OSCC. It may also be useful to conduct further studies on the use of E2F2 expression profile changes as a factor to describe the invasiveness and dynamics of OSCC development.

Type of Medium: Online Resource

ISSN: 2227-9059

URL: Article

DOI: 10.3390/biomedicines10123011

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2720867-9

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Evaluation of Malondialdehyde Level, Total Oxidant/Antioxidant Status and Oxidative Stress Index in Colorectal Cancer Patients

Janion, Karolina ; Strzelczyk, Joanna Katarzyna ; Walkiewicz, Katarzyna Weronika ; [et al.]

MDPI AG ; 2022

In: Metabolites Vol. 12, No. 11 ( 2022-11-15), p. 1118-

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In: Metabolites, MDPI AG, Vol. 12, No. 11 ( 2022-11-15), p. 1118-

Abstract: Oxidative stress may play an important role in colorectal cancer (CRC). The present study included 94 adult patients with CRC (52 men and 42 women) and 26 hospitalized patients (12 men and 14 women) in whom CRC was excluded (control group). During hospitalization, blood serum samples were collected from both groups. Apart from that, anthropometric measurements were taken and other clinical data were analyzed. Serum malondialdehyde (MDA) level, total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) were assayed. Subsequently, the relationship between the analyzed oxidative stress markers and selected clinical characteristics was investigated in both groups. The evaluation of oxidative stress marker values demonstrated that MDA and TAS levels were significantly higher in the control group than the CRC group (p 〈 0.001 and p = 0.019, respectively), while TOS levels were significantly higher in the CRC group than the control group (p = 0.005). Significantly lower OSI levels were found in the control group than in the CRC group (p 〈 0.001). Similar results can be observed when performing ROC analysis (receiver operating characteristic curve). Preliminary statistical analysis demonstrated that MDA levels in the study group depend on the location of the primary tumour (p = 0.035). Based on the post hoc Tukey test, a relationship was demonstrated between the MDA level and the left and right side of the colon (p = 0.040). The results may be evidence for a higher level of oxidative stress, including a compromised antioxidative defence system, in patients with CRC.

Type of Medium: Online Resource

ISSN: 2218-1989

URL: Article

DOI: 10.3390/metabo12111118

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2662251-8

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Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer

Gaździcka, Jadwiga ; Gołąbek, Karolina ; Strzelczyk, Joanna Katarzyna ; [et al.]

Polskie Towarzystwo Biochemiczne (Polish Biochemical Society) ; 2020

In: Acta Biochimica Polonica ( 2020-05-07)

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In: Acta Biochimica Polonica, Polskie Towarzystwo Biochemiczne (Polish Biochemical Society), ( 2020-05-07)

Abstract: The head and neck squamous cell carcinoma (HNSCC) is an aggressive human malignancy diagnosed in more than 800 000 new cases worldwide, and mostly arises in the oral cavity, oropharynx, larynx, hypopharynx, and nasopharynx. The study presented here aimed to determine a possible association of rs11515 and rs3088440 gene polymorphisms in the CDKN2A gene (cyclin dependent kinase inhibitor 2A), as well as rs769412 and rs937283 in the MDM2 gene (murine double minute 2), with oral cavity cancer in a sample of Polish population; CDKN2A is crucial in regulating the cell cycle while MDM2 is an oncogene involved in regulating cell proliferation and apoptosis. The study included 95 primary tumor samples following surgical resection from patients, and the control group consisted of 100 healthy individuals. DNA samples were genotyped by employing the 5’ nuclease assay for allelic discrimination using TaqMan SNP Genotyping Assays (Applied Biosystems, USA). There was no significant association between any of the polymorphism (rs11515, rs3088440, rs769412 and rs937283) and the oral cavity cancer risk. We found that the AA homozygotes for rs3088440 were significantly more frequent in the control group (OR=0.046, p 〈 0.0001). In addition, the GG genotype of rs769412 was not found in any group. We found no influence of the examined genotypes on clinicopathological parameters, such as T, N and grading values in patients with oral cavity cancer. The results of this study indicate that none of the investigated polymorphisms were associated with the risk of oral cavity cancer in the examined sample of the Polish population.

Type of Medium: Online Resource

ISSN: 1734-154X , 0001-527X

URL: Article

DOI: 10.18388/abp.2020_5195

Language: Unknown

Publisher: Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)

Publication Date: 2020

detail.hit.zdb_id: 2086022-5

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Assessment of CMKLR1 level in colorectal cancer and its correlation with angiogenic markers

Kiczmer, Paweł ; Seńkowska, Alicja Prawdzic ; Kula, Agnieszka ; [et al.]

Elsevier BV ; 2020

In: Experimental and Molecular Pathology Vol. 113 ( 2020-04), p. 104377-

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In: Experimental and Molecular Pathology, Elsevier BV, Vol. 113 ( 2020-04), p. 104377-

Type of Medium: Online Resource

ISSN: 0014-4800

URL: Article

DOI: 10.1016/j.yexmp.2020.104377

Language: English

Publisher: Elsevier BV

Publication Date: 2020

detail.hit.zdb_id: 1466769-1

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