In:
ChemBioChem, Wiley, Vol. 20, No. 20 ( 2019-10-15), p. 2593-2596
Abstract:
The regeneration of enzymatic cofactors by cell‐free synthetic modules is a key step towards producing a purely synthetic cell. Herein, we demonstrate the regeneration of the enzyme cofactor NAD + by photo‐oxidation of NADH under visible‐light irradiation by using metal‐free conjugated polymer nanoparticles. Encapsulation of the light‐active nanoparticles in the lumen of polymeric vesicles produced a fully organic module able to regenerate NAD + in an enzyme‐free system. The polymer compartment conferred physical and chemical autonomy to the module, allowing the regeneration of NAD + to occur efficiently, even in harsh chemical environments. Moreover, we show that regeneration of NAD + by the photocatalyst nanoparticles can oxidize a model substrate, in conjunction with the enzyme glycerol dehydrogenase. To ensure the longevity of the enzyme, we immobilized it within a protective silica matrix; this yielded enzymatic silica nanoparticles with enhanced long‐term performance and compatibility with the NAD + ‐regeneration system.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.201900093
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2020469-3
SSG:
12
Bookmarklink