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  • 1
    In: Cell Transplantation, SAGE Publications, Vol. 19, No. 10 ( 2010-10), p. 1281-1290
    Abstract: Stem cell (SC) therapy represents a promising approach to treat a wide variety of injuries, inherited diseases, or acquired SC deficiencies. One of the major problems associated with SC therapy remains the absence of a suitable matrix for SC growth and transfer. We describe here the growth and metabolic characteristics of mouse limbal stem cells (LSCs) and mesenchymal stem cells (MSCs) growing on 3D nanofiber scaffolds fabricated from polyamide 6/12 (PA6/12). The nanofibers were prepared by the original needleless electrospun Nanospider technology, which enables to create nanofibers of defined diameter, porosity, and a basis weight. Copolymer PA6/12 was selected on the basis of the stability of its nanofibers in aqueous solutions, its biocompatibility, and its superior properties as a matrix for the growth of LSCs, MSCs, and corneal epithelial and endothelial cell lines. The morphology, growth properties, and viability of cells grown on PA6/12 nanofibers were comparable with those grown on plastic. LSCs labeled with the fluorescent dye PKH26 and grown on PA6/12 nanofibers were transferred onto the damaged ocular surface, where their seeding and survival were monitored. Cotransfer of LSCs with MSCs, which have immunosuppressive properties, significantly inhibited local inflammatory reactions and supported the healing process. The results thus show that nanofibers prepared from copolymer PA6/12 represent a convenient scaffold for growth of LSCs and MSCs and transfer to treat SC deficiencies and various ocular surface injuries.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2010
    detail.hit.zdb_id: 1135816-6
    detail.hit.zdb_id: 2020466-8
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  • 2
    In: Chemosphere, Elsevier BV, Vol. 280 ( 2021-10), p. 130749-
    Type of Medium: Online Resource
    ISSN: 0045-6535
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 120089-6
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  • 3
    In: Environmental and Molecular Mutagenesis, Wiley, Vol. 47, No. 7 ( 2006-08), p. 533-540
    Abstract: Synthetic dyes are released in wastewater from textile manufacturing plants, and many of these dyes are genotoxic. In the present study, the mutagenicity of azo, anthraquinone, and triphenyl methane dyes was investigated before and after successive biodegradation with activated sludge and the ligninolytic fungus, Irpex lacteus. Two biodegradation systems were used to reduce the genotoxicity of dyes that were not efficiently inactivated by activated sludge alone. Mutagenicity was monitored with the Salmonella reversion assay conducted with the base‐pair substitution detector strains, TA100 and YG1042, and the frame‐shift detector strains, TA98 and YG1041, with and without rat liver S9. All dyes except for Congo Red (CR) were mutagenic with S9 activation. Assays conducted with the dyes indicated that only the azo dye Reactive Orange 16 (RO16) was mutagenic in both TA98 and TA100. Methyl Red and Disperse Blue 3 (DB3) were mutagenic in TA98, YG1041 and YG1042, while Reactive Black 5 was mutagenic in YG1041 and YG1042. Remazol Brilliant Blue R (RBBR), Crystal violet (CV) and Bromophenol Blue (BPB) were mutagenic only in TA98, but the toxicity of the latter two dyes complicated the evaluation of their mutagenicity. CR was not mutagenic in any of the tester strains. Biodegradation studies conducted with RO16 and DB3 indicated that the two‐step biodegradation process reduced the mutagenic potential of RO16 and DB3 to a greater extent than activated sludge alone; the mutagenicity of the two dyes was reduced by 95.2% and 77.8%, respectively, by the two‐step process. These data indicate that the combined biodegradation process may be useful for reducing the mutagenicity associated with wastewater from textile factories that contain recalcitrant dyes. Environ. Mol. Mutagen., 2006. © 2006 Wiley‐Liss, Inc.
    Type of Medium: Online Resource
    ISSN: 0893-6692 , 1098-2280
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2006
    detail.hit.zdb_id: 1497682-1
    detail.hit.zdb_id: 639145-X
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Wiley ; 2018
    In:  Journal of Plant Nutrition and Soil Science Vol. 181, No. 1 ( 2018-02), p. 31-35
    In: Journal of Plant Nutrition and Soil Science, Wiley, Vol. 181, No. 1 ( 2018-02), p. 31-35
    Abstract: Quantifying and understanding fluxes of methane (CH 4 ) and carbon dioxide (CO 2 ) in natural soil–plant–atmosphere systems are crucial to predict global climate change. Wetland herbaceous species or tree species at waterlogged sites are known to emit large amounts of CH 4 . Upland forest soils are regarded as CH 4 sinks and tree species like upland beech are not known to significantly emit CH 4 . Yet, data are scarce and this assumption needs to be tested. We combined measurements of soil–atmosphere and stem–atmosphere fluxes of CO 2 and CH 4, and soil gas profiles to assess the contribution of the different ecosystem compartments at two upland beech forest sites in Central Europe in a case study. Soil was a net CH 4 sink at both sites, though emissions were detected consistently from beech stems at one site. Although stem emissions from beech stems were high compared to known fluxes from other upland tree species, they were substantially lower compared to the strong CH 4 sink of the soil. Yet, we observed extraordinarily large CH 4 emissions from one beech tree that was 140% of the CH 4 sink of the soil. The soil gas profile at this tree indicated CH 4 production at a soil depth 〉 0.3 m, despite the net uptake of CH 4 consistently observed at the soil surface. Field soil assessment showed strong redoximorphic color patterns in the adjacent soil and supports this evaluation. We hypothesize that there is a transport link between the soil and stem via the root system representing a preferential transport mechanism for CH 4 despite the fact that beech roots usually do not bear aerenchyma. The high mobility of gases requires a holistic view on the soil–plant–atmosphere system. Therefore, we recommend including field soil assessment and soil gas profiles measurements when investigating soil–atmosphere and stem–atmosphere fluxes to better understand the sources of gases and their transport mechanisms.
    Type of Medium: Online Resource
    ISSN: 1436-8730 , 1522-2624
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1481142-X
    detail.hit.zdb_id: 1470765-2
    SSG: 12
    SSG: 13
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  • 5
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2017
    In:  Scientific Reports Vol. 7, No. 1 ( 2017-10-16)
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-10-16)
    Abstract: Naturally produced by microbial processes in soil, nitrous oxide (N 2 O) is an important greenhouse gas contributing to climate change. Accordingly, there is a need to accurately quantify the capability of forest ecosystems to exchange N 2 O with the atmosphere. While N 2 O emissions from soils have been well studied, trees have so far been overlooked in N 2 O inventories. Here, we show that stems of mature beech trees ( Fagus sylvatica ) may act as a substantial sink of N 2 O from the atmosphere under conditions of soils consuming N 2 O. Consistent consumption of N 2 O by all stems investigated (ranging between −2.4 and −3.8 µg m −2 h −1 ) is a novel finding in contrast to current studies presenting trees as N 2 O emitters. To understand these fluxes, N 2 O exchange of photoautotrophic organisms associated with beech bark (lichens, mosses and algae) was quantified under laboratory conditions. All these organisms were net N 2 O sinks at full rehydration and temperature of 25 °C. The consumption rates were comparable to stem consumption rates measured under field conditions. Cryptogamic stem covers could be a relevant sink of N 2 O in European beech forests.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2615211-3
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  • 6
    In: Processes, MDPI AG, Vol. 11, No. 1 ( 2023-01-13), p. 261-
    Abstract: The aldol condensation of furfural and acetone is considered a promising method for the production of liquid fuel intermediates. 4-(2-furyl)-3-buten-2-one (FAc) and 1,5-di-2-furanyl-1,4-pentadien-3-one (F2Ac) are the main products of the reaction, which can go through the hydrodeoxygenation process to convert to diesel and jet fuel range fuels. Considering the present situation at the fuel-market related to crude oil shortage, the above-mentioned process seems to be a convenient path to obtain fuels in the diesel and kerosene range. This research focuses on the effect of water on the furfural conversion and product distribution during the aldol condensation. The catalyst chosen for this research was MgAl mixed oxide in molar ratio 3:1. The reaction was performed at 40 °C and 1 MPa in a continuous-flow reactor with and without water in the feedstock. The physicochemical properties of the catalyst were evaluated using different techniques. The catalyst lifetime decreased and the catalyst deactivation started faster by the addition of 5 wt.% water to the feedstock with the furfural to acetone ratio (F:Ac) of 1:2.5. Selectivity to FAc increased by 10% in the presence of water. The catalyst lifetime enhanced by increasing the F:Ac ratio from 1:2.5 to 1:5, in the presence of 5 wt.% water. The furfural conversion was 100% after 28 h of reaction, and then decreased gradually to 40% after 94 h of reaction. At higher F:Ac ratio, the selectivity to FAc was 10% higher, while the F2Ac was about 8% lower.
    Type of Medium: Online Resource
    ISSN: 2227-9717
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2720994-5
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  • 7
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 78, No. 13_Supplement ( 2018-07-01), p. 1012-1012
    Abstract: The hallmark of chronic lymphocytic leukemia (CLL) cells is their re-circulation between peripheral blood and immune niches to obtain pro-proliferative and pro-survival signals. CLL cells that have recently exited the immune niches to the peripheral blood are characterized by low cell-surface levels of chemokine receptor CXCR4 and high levels of activation molecule CD5. These CXCR4dimCD5bright CLL cells have a ~2-fold higher CD20 expression due to the activation of the CXCR4/SDF-1 axis (Pavlasova et al., Blood, 2016). We hypothesized that CD20 up-regulation in the context of a microenvironment is required for some functional regulation. We hypothesized that CD20 expression is of importance for B-cell receptor (BCR) signaling as we observed that CXCR4dimCD5brightCD20bright CLL cells have also ~2-fold higher surface IgM levels (P & lt;0.0001). This was coupled with higher responsiveness to BCR-crosslinking with anti-IgM (P=0.0015). CD20 levels directly affect BCR-induced calcium flux and the phosphorylation of BCR/PI3K-associated molecules (LYN, SYK, ERK, GAB1) after BCR-crosslinking. The CXCR4dimCD5brightCD20bright subpopulation contains more proliferative (Ki67+) cells, higher levels of pAKT/pERK/pCD79a (P & lt;0.001), and their gene expression signature (NGS Illumina) is significantly enriched for genes involved in BCR and MAPK signaling, migration, and actin cytoskeleton organization (P & lt;0.0001). Finally, we have shown that rituximab primarily and potently eliminates the CXCR4dimCD5brightCD20bright CLL cells (P & lt;0.0001). Overall, rituximab was ~9-fold more efficient in eliminating CXCR4dimCD5brightCD20bright CLL cells than CXCR4brightCD5dimCD20dim cells (P=0.03) during FCR therapy in vivo. Altogether, we described that higher CD20 expression supports BCR signaling and contributes to the activated phenotype and aggressiveness of an intra-clonal subpopulation of CXCR4dimCD5brightCD20bright cells. This is a first mechanistic explanation of CD20 function in CLL cells. Additionally, it is tempting to speculate that rituximab's clinical success is at least partially attributed to the preferential elimination of the intra-clonal proliferative subpopulation of BCR-proficient CLL cells. Supported by: the Ministry of Education, Youth and Sports of the Czech Rep. under the project CEITEC 2020 (LQ1601); Czech Science Foundation (project No. 16-13334Y); the Ministry of Health of the Czech Rep., grant No. 16-29622A. All rights reserved. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 692298. This study reflects only the author's view and the Research Executive Agency is not responsible for any use that may be made of the information it contains. MH CZ-DRO (FNBr, 65269705); MUNI/H/0865/2016; MUNI/A/0968/2017. Citation Format: Gabriela Pavlasova, Marek Borsky, Veronika Svobodova, Jan Oppelt, Katerina Cerna, Jitka Novotna, Katerina Musilova, Vaclav Seda, Eva Vojackova, Yvona Brychtova, Michael Doubek, Sarka Pospisilova, Jiri Mayer, Marek Mraz. CD20 supports BCR signaling in an intra-clonal aggressive chronic lymphocytic leukemia subpopulation of cells and rituximab primarily targets these BCR-proficient B cells in vivo [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1012.
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    RVK:
    RVK:
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2018
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
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  • 8
    In: Bioresource Technology, Elsevier BV, Vol. 114 ( 2012-06), p. 241-246
    Type of Medium: Online Resource
    ISSN: 0960-8524
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
    detail.hit.zdb_id: 1065195-0
    SSG: 12
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  • 9
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 77, No. 13_Supplement ( 2017-07-01), p. 1479-1479
    Abstract: MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression, and are frequently aberrantly expressed in cancer. We aimed to understand their role in the transformation of indolent follicular lymphoma (FL) into an aggressive diffuse large B cell lymphoma. This happens in ~3% of cases per year during the course of the disease, and is associated with median survival of only 2 years. The NGS revealed number of aberrations associated with transformed FL (tFL), including frequent high-level activity of MYC (amplifications, translocations, and mutations) or loss of DNA damage regulators (p53, CDKN2A/B). Firstly, we performed a miRNA profiling (TaqMan miRNA Arrays) in paired FL and tFL samples (N=8 pairs). This revealed a relatively small group of 5 miRNAs that are consistently differentially expressed in tFL (P & lt;0.05, fold-change & gt;1.5). Since the most frequently acquired aberration in tFL is the high-level activity of MYC we performed a correlation analysis of MYC levels and expression of these miRNAs in additional samples of FL, tFL, and CLL samples with/without MYC duplication (N=40 FL/tFL, N=39 CLL). This revealed that at least one of these miRNAs is significantly down-modulated (P & lt;0.05) in cases with high-levels of MYC. The MYC-mediated repression of miRNA levels was also observed (P & lt;0.05) in B cells from transgenic MYC over-expressing mice (MYC controlled by an Ig-alpha enhancer) in comparison to wild-type animals (samples obtained from young animals before occurrence of any malignancy). We have further shown that the levels of this miRNA affect B cell proliferation in vitro, and its low-levels associate with percentage of Ki67 positive cells in FL samples (P & lt;0.005). Moreover, low levels of tFL-associated miRNA were present in FL cases with a shorter overall survival (P & lt;0.01), and its expression directly affected BCR signalling (calcium flux assay after anti-IgM). We have shown that the expression of this miRNA is not only down-modulated by high-level MYC expression, but also by B cell adhesion to stromal cells in co-culture in vitro (HS-5 stromal cells). This suggests that its normal physiological function might be related to regulation of B cell functions in the context of immune niches, and this might play a role in FL progression and transformation. It remains to be elucidated what other molecular mechanisms ensure low-level expression of the studied miRNA in cases that do not harbor MYC over-expression, and what pool of target mRNAs is regulated by this miRNA in FL cells.This work was supported by: the Ministry of Health of the Czech Republic, grant nr. 16-29622A. All rights reserved. contact: marek.mraz@email.cz Citation Format: Katerina Musilova, Gabriela Pavlasova, Vaclav Seda, Eva Vojackova, Katerina Cerna, Veronika Svobodova, Robert Pytlik, Vit Prochazka, Zuzana Prouzova, Sarka Pospisilova, Lenka Zlamalikova, Heidi Mocikova, Lenka Kruzova, Marie Jarosova, Andrew Evans, Clive Zent, Leos Kren, Marek Trneny, Jiri Mayer, Andrea Janikova, Marek Mraz. Differential expression of microRNAs in transformation of follicular lymphoma to diffuse large B cell lymphoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1479. doi:10.1158/1538-7445.AM2017-1479
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    RVK:
    RVK:
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2017
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
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  • 10
    Online Resource
    Online Resource
    MDPI AG ; 2024
    In:  Materials Vol. 17, No. 3 ( 2024-01-31), p. 685-
    In: Materials, MDPI AG, Vol. 17, No. 3 ( 2024-01-31), p. 685-
    Abstract: Elevated concentrations of heavy metals in natural waters can cause significant ecological problems. It is therefore essential to ensure their removal from any water discharged into the environment immediately, especially in case of an accident, where there is a risk of releasing large quantities or high concentrations. The aim of this paper is to test a newly developed adsorbent for the removal of heavy metals from aqueous solutions—in particular, it is very fast adsorption, and thus efficiency, during clean-ups. The alkali-activated foamed zeolite adsorbent was laboratory-prepared and -tested in both batch and flow-through arrangements on single and multi-component solutions and compared with natural zeolite. The experimental setup for batch adsorption consisted of a set of samples and solutions containing iron, cobalt, manganese, zinc and nickel. The samples were put on a horizontal shaker with a 500 mg adsorbent loading in a 50 mL solution. The column adsorption experimental setup consisted of a glass column with an inside diameter of 15 mm and a bed length of 165 mm. A measured amount of each adsorbent was added to the column to achieve a filter fixed-bed height of 160 mm. The high efficiency of the tested adsorbent on various heavy metals was confirmed. The adsorbent has a high potential for use in decontamination processes, water protection and landscape revitalization. Due to its rapid precipitation and subsequent fixation of metal cations in the form of insoluble oxide or hydroxide, it can be used as an emergency adsorbent, the great advantage of which is its low production cost and natural origin.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    Language: English
    Publisher: MDPI AG
    Publication Date: 2024
    detail.hit.zdb_id: 2487261-1
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