In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 52, No. 3 ( 2008-03), p. 837-845
Abstract:
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, which often produce Panton-Valentine leucocidin (PVL), are increasingly noted worldwide. In this study, we examined 42 MRSA strains (25 PVL-positive [PVL + ] strains and 17 PVL-negative [PVL − ] strains) isolated in Taiwan for their molecular characteristics. The PVL + MRSA strains included CA-MRSA strains with multilocus sequence type (ST) 59 (major PVL + MRSA in Taiwan), its variants, and worldwide CA-MRSA ST30 strains. The PVL − MRSA strains included the pandemic Hungarian MRSA ST239 strain, the Hungarian MRSA ST239 variant, MRSA ST59 (largely hospital-acquired MRSA strains) and its variants, the pandemic New York/Japan MRSA ST5 strain (Japanese type), and the MRSA ST8 strain. The major PVL + CA-MRSA ST59 strain possessed a tetracycline resistance-conferring ( tetK positive) penicillinase plasmid and a drug resistance gene cluster (a possible composite transposon) for multidrug resistance. Moreover, it carried a novel staphylococcal cassette chromosome mec (SCC mec ) with two distinct ccrC genes ( ccrC2 - C8 ). This SCC mec (previously named SCC mec type V T ) was tentatively designated SCC mec type VII. Sequencing of the PVL genes revealed the polymorphisms, and the PVL + CA-MRSA ST59 strain possessed the ST59-specific PVL gene sequence. The data suggest that a significant amount of clonal spread is occurring in Taiwan and that the major PVL + CA-MRSA ST59 Taiwan strain exhibits unique genetic characteristics, such as a novel SCC mec type and an ST59-specific PVL gene sequence.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.01001-07
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2008
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
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