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  • 1
    Online Resource
    Online Resource
    Transcriptome provides potential insights into how calcium affects the formation of stone cell in Pyrus
    Springer Science and Business Media LLC ; 2021
    In:  BMC Genomics Vol. 22, No. 1 ( 2021-12)
    Details
    In: BMC Genomics, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2021-12)
    Abstract: The content of stone cells in pears has a great influence on taste. Stone cells are formed by the accumulation of lignin. The treatment of exogenous calcium can affect the lignin synthesis, but this Ca-mediated mechanism is still unclear. In this study, the author performed a comparative transcriptomic analysis of callus of pears ( Pyrus x bretschneideri ) treated with calcium nitrate Ca (NO 3 ) 2 to investigate the role of calcium in lignin synthesis. Results There were 2889 differentially expressed genes (DEGs) detected between the Control and Ca (NO 3 ) 2 treatment in total. Among these 2889 DEGs, not only a large number of genes related to Ca single were found, but also many genes were enriched in secondary metabolic pathway, especially in lignin synthesis. Most of them were up-regulated during the development of callus after Ca (NO 3 ) 2 treatment. In order to further explore how calcium nitrate treatment affects lignin synthesis, the author screened genes associated with transduction of calcium signal in DEGs, and finally found CAM, CML, CDPK, CBL and CIPK. Then the author identified the PbCML3 in pears and conducted relevant experiments finding the overexpression of PbCML3 would increase the content of pear stone cells, providing potential insights into how Ca treatment enhances the stone cell in pears. Conclusions Our deep analysis reveals the effects of exogenous calcium on calcium signal and lignin biosynthesis pathway. The function of PbCML3 on stone cells formation was verified in pear.
    Type of Medium: Online Resource
    ISSN: 1471-2164
    URL: Article
    DOI: 10.1186/s12864-021-08161-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2041499-7
    SSG: 12
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  • 2
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    Online Resource
    Study of the Distribution Characteristics of the Airflow Field in Tree Canopies Based on the CFD Model
    MDPI AG ; 2022
    In:  Agronomy Vol. 12, No. 12 ( 2022-12-04), p. 3072-
    Details
    In: Agronomy, MDPI AG, Vol. 12, No. 12 ( 2022-12-04), p. 3072-
    Abstract: Air-assisted sprayers are the mainstream orchard plant protection machinery in China. During the usage of sprayers, the pesticide droplets carried by strong air jets from sprayers enter into the target canopy. Therefore, the distribution of airflow field in the canopy has significant influence on the spatial movement of the droplets and the adhesion and penetration of the droplets inside the canopy. To enhance the working performance of sprayers, it is imperative to study their use in tree canopies. Based on computational fluid dynamics (CFD), the k-ε turbulence model, and the SIMPLE algorithm, a 3D simulated model of the spatial distribution of the airflow field in and around the tree canopy was established based on the porous model in this paper. The model was used to simulate and calculate the air field distribution of an air-assisted orchard sprayer under different operating parameters. The results showed that the optimal operation effect was achieved when the driving speed and the air speed of the fan outlet were 1 m/s and 20 m/s, respectively, while the air speed in the canopy was not less than 2 m/s. The 36 points measured in the canopy were compared with the simulated results through field experiments. It showed that average relative error between the measured and simulated values was 13.85%, and the overall goodness-of-fit was 0.97656. The model accurately simulated the airflow distribution in the canopy and provided a basis for optimizing the operating parameters of the air-assisted sprayers in orchards.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    URL: Article
    DOI: 10.3390/agronomy12123072
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 3
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    Online Resource
    Ornithine and breast cancer: a matched case–control study
    Springer Science and Business Media LLC ; 2020
    In:  Scientific Reports Vol. 10, No. 1 ( 2020-09-23)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-09-23)
    Abstract: In vivo and vitro evidence indicates that ornithine and its related metabolic products play a role in tumor development. Whether ornithine is associated with breast cancer in humans is still unclear. We examined the association between circulating ornithine levels and breast cancer in females. This 1:1 age-matched case–control study identified 735 female breast cancer cases and 735 female controls without breast cancer. All cases had a pathological test to ascertain a breast cancer diagnosis. The controls were ascertained using pathologic testing, clinical examinations, and/or other tests. Fasting blood samples were used to measure ornithine levels. The average age for cases and controls were 49.6 years (standard deviation [SD] 8.7 years) and 48.9 years (SD 8.7 years), respectively. Each SD increase in ornithine levels was associated with a 12% reduction of breast cancer risk (adjusted odds ratio [OR] 0.88; 95% confidence interval [CI] 0.79–0.97). The association between ornithine and breast cancer did not differ by pathological stages of diagnosis or tumor grades (all P for trend  〉  0.1). We observed no effect measure modification by molecular subtypes ( P for interaction = 0.889). In conclusion, higher ornithine levels were associated with lower breast cancer risk in females.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-020-72699-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2615211-3
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  • 4
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    Online Resource
    PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
    Springer Science and Business Media LLC ; 2021
    In:  Scientific Reports Vol. 11, No. 1 ( 2021-05-03)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-05-03)
    Abstract: Pear [ Pyrus bretschneideri cv. Dangshan Su] fruit quality is not always satisfactory owing to the presence of stone cells, and lignin is the main component of stone cells in pear fruits. Caffeoyl shikimate esterase (CSE) is a key enzyme in the lignin biosynthesis. Although CSE -like genes have been isolated from a variety of plant species, their orthologs are not characterized in pear. In this study, the CSE gene family ( PbCSE ) from P. bretschneideri was identified. According to the physiological data and quantitative RT-PCR (qRT-PCR), PbCSE1 was associated with lignin deposition and stone cell formation. The overexpression of PbCSE1 increased the lignin content in pear fruits. Relative to wild-type (WT) Arabidopsis , the overexpression of PbCSE1 delayed growth, increased the lignin deposition and lignin content in stems. Simultaneously, the expression of lignin biosynthetic genes were also increased in pear fruits and Arabidopsis . These results demonstrated that PbCSE1 plays an important role in cell lignification and will provide a potential molecular strategy to improve the quality of pear fruits.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-021-88825-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2615211-3
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  • 5
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    Online Resource
    The genome of the pear ( Pyrus bretschneideri Rehd.)
    Cold Spring Harbor Laboratory ; 2013
    In:  Genome Research Vol. 23, No. 2 ( 2013-02), p. 396-408
    Details
    In: Genome Research, Cold Spring Harbor Laboratory, Vol. 23, No. 2 ( 2013-02), p. 396-408
    Abstract: The draft genome of the pear ( Pyrus bretschneideri ) using a combination of BAC-by-BAC and next-generation sequencing is reported. A 512.0-Mb sequence corresponding to 97.1% of the estimated genome size of this highly heterozygous species is assembled with 194× coverage. High-density genetic maps comprising 2005 SNP markers anchored 75.5% of the sequence to all 17 chromosomes. The pear genome encodes 42,812 protein-coding genes, and of these, ∼28.5% encode multiple isoforms. Repetitive sequences of 271.9 Mb in length, accounting for 53.1% of the pear genome, are identified. Simulation of eudicots to the ancestor of Rosaceae has reconstructed nine ancestral chromosomes. Pear and apple diverged from each other ∼5.4–21.5 million years ago, and a recent whole-genome duplication (WGD) event must have occurred 30–45 MYA prior to their divergence, but following divergence from strawberry. When compared with the apple genome sequence, size differences between the apple and pear genomes are confirmed mainly due to the presence of repetitive sequences predominantly contributed by transposable elements (TEs), while genic regions are similar in both species. Genes critical for self-incompatibility, lignified stone cells (a unique feature of pear fruit), sorbitol metabolism, and volatile compounds of fruit have also been identified. Multiple candidate SFB genes appear as tandem repeats in the S -locus region of pear; while lignin synthesis-related gene family expansion and highly expressed gene families of HCT , C3′H , and CCOMT contribute to high accumulation of both G-lignin and S-lignin. Moreover, alpha-linolenic acid metabolism is a key pathway for aroma in pear fruit.
    Type of Medium: Online Resource
    ISSN: 1088-9051
    URL: Article
    DOI: 10.1101/gr.144311.112
    RVK:
    XA 10000
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2013
    detail.hit.zdb_id: 1483456-X
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)
    Elsevier BV ; 2016
    In:  Scientia Horticulturae Vol. 206 ( 2016-07), p. 33-42
    Details
    In: Scientia Horticulturae, Elsevier BV, Vol. 206 ( 2016-07), p. 33-42
    Type of Medium: Online Resource
    ISSN: 0304-4238
    URL: Article
    DOI: 10.1016/j.scienta.2016.04.019
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2016
    detail.hit.zdb_id: 2016351-4
    SSG: 12
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  • 7
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    Online Resource
    Mitochondrial dysfunction mediated by cytoplasmic acidification results in pollen tube growth cessation in Pyrus pyrifolia
    Wiley ; 2015
    In:  Physiologia Plantarum Vol. 153, No. 4 ( 2015-04), p. 603-615
    Details
    In: Physiologia Plantarum, Wiley, Vol. 153, No. 4 ( 2015-04), p. 603-615
    Abstract: The length of pollen tubes grown in synthetic media is normally shorter than those grown in vivo. However, the mechanism(s) underlying the cessation of pollen tube growth under culture conditions remain(s) largely unknown. Here, we report a previously unknown correlation between vacuolar function and the cell's ability to sustain mitochondrial functions in pear pollen tubes. The pear pollen tubes in vitro grew slowly after 15 hours post‐cultured ( HPC ) and nearly ceased growth at 18 HPC . There was increased malondialdehyde content and membrane ion leakage at 15 HPC compared with 12 HPC . Furthermore, cytoplasmic acidification mainly mediated by decreased vacuolar H + ‐ ATP ase [ V‐ATP ase, Enzyme Commission (EC) 3.6.1.3] activity was observed in pollen tubes after 15 HPC , and this further resulted in mitochondrial dysfunction, including mitochondrial structure disruption, mitochondrial membrane potential collapse and decreases in both oxygen consumption and ATP production. Our findings suggest that vacuoles and mitochondria intimately linked in regulating pollen tube elongation.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.2015.153.issue-4
    DOI: 10.1111/ppl.12260
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 8
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    Online Resource
    Correlation between ABO blood group and prognosis of hepatectomy for hepatitis B virus-associated hepatocellular carcinoma
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  European Journal of Gastroenterology & Hepatology Vol. 35, No. 9 ( 2023-09), p. 1012-1022
    Details
    In: European Journal of Gastroenterology & Hepatology, Ovid Technologies (Wolters Kluwer Health), Vol. 35, No. 9 ( 2023-09), p. 1012-1022
    Abstract: The relationship between ABO blood group and prognosis of patients with hepatocellular carcinoma (HCC) remains unclear. We investigated the relationship between prognosis and ABO blood group in patients with hepatitis B-associated HCC after radical hepatectomy. Methods The medical records of 874 patients with hepatitis B-associated HCC who underwent radical liver tumor resection were retrospectively collected. Cox proportional risk models were constructed for analysis, and the patient data were further balanced using propensity score matching (PSM) analysis to assess the impact of ABO blood group on the prognosis of patients with hepatitis B-associated HCC. Results In univariate Cox regression analysis, the overall survival (OS) of non-A blood type group vs. A blood type group [hazard ratio (HR) (95% confidence interval [CI]) = 1.504 (1.003–2.255), P  = 0.048], in multivariate Cox regression analysis the OS of non-A blood type group versus A blood type group [HR (95% CI) = 1.596 (1.054–2.417), P  = 0.027]. After PSM, the baseline information was more balanced between the two groups, yielding the same results as above [HR (95% CI) = 1.550 (1.012–2.373), P  = 0.044]. Conclusion The difference in OS after radical hepatectomy in patients with hepatitis B-associated HCC was statistically significant in terms of ABO blood group, OS was lower in patients with non-A blood group than in patients with A blood group.
    Type of Medium: Online Resource
    ISSN: 0954-691X
    URL: Article
    DOI: 10.1097/MEG.0000000000002593
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2023
    detail.hit.zdb_id: 2030291-5
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  • 9
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    Online Resource
    Cinnamate-4-Hydroxylase Gene Is Involved in the Step of Lignin Biosynthesis in Chinese White Pear
    American Society for Horticultural Science ; 2015
    In:  Journal of the American Society for Horticultural Science Vol. 140, No. 6 ( 2015-11), p. 573-579
    Details
    In: Journal of the American Society for Horticultural Science, American Society for Horticultural Science, Vol. 140, No. 6 ( 2015-11), p. 573-579
    Abstract: Lignin is the main component of stone cells, and stone cell content is one of the crucial factors for fruit quality in chinese white pear ( Pyrus × bretschneideri ). The lignin biosynthesis pathway is complex and involves many enzymatic reactions. Cinnamate-4-hydroxylase [C4H (EC.1.14.13.11)] is an essential enzyme in lignin metabolism. This study was conducted to investigate the effect of bagging on lignin metabolism during fruit development in chinese white pear. The study showed that bagging had little effect on stone cell content, lignin content, C4H activity, and C4H gene expression and that there was a positive correlation between C4H gene expression and lignin content as well as stone cell content. Moreover, a full-length complementary DNA (cDNA) encoding C4H ( PbrC4H , GenBank accession number KJ577541.1) was isolated from chinese white pear fruit. The cDNA is 1515 bp long and encodes a protein of 504 amino acids. Sequence alignment suggested that the deduced protein belongs to the P450 gene family and that C4H might be located subcellularly in the cell membrane. The results indicate that bagging cannot change the lignin and stone cell content significantly and that C4H catalyzes a step in lignin biosynthesis. These findings provide certain theoretical references and practical criteria for improving the quality of chinese white pear.
    Type of Medium: Online Resource
    ISSN: 0003-1062 , 2327-9788
    URL: Article
    DOI: 10.21273/JASHS.140.6.573
    Language: Unknown
    Publisher: American Society for Horticultural Science
    Publication Date: 2015
    detail.hit.zdb_id: 2040057-3
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  • 10
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    Online Resource
    Identification and Characterization of the CCoAOMT Gene Family in Apple, Chinese White Pear, and Peach
    American Society for Horticultural Science ; 2021
    In:  Journal of the American Society for Horticultural Science Vol. 146, No. 3 ( 2021-05), p. 184-195
    Details
    In: Journal of the American Society for Horticultural Science, American Society for Horticultural Science, Vol. 146, No. 3 ( 2021-05), p. 184-195
    Abstract: Lignin is one of the main components of plant cell walls, which provides mechanical support for plants and also contributes to resisting against plant pathogenic fungi. In the fruit industry, the lignin content can affect the quality of fruit. The biosynthesis of lignin involves a variety of enzymes, of which caffeoyl-CoA 3-O-methyltransferase ( CCoAOMT ) is a class of methyltransferases that plays an essential role in lignin biosynthesis. Studies have been conducted on the CCoAOMT gene family in several species, including arabidopsis ( Arabidopsis thaliana ), black poplar ( Populus nigra ), and cotton ( Gossypium hirsutum ). Still, there is relatively little research on this gene family in the Rosaceae. In this study, we used bioinformatics to identify and characterize the CCoAOMT gene family in apple ( Malus domestica ), chinese white pear ( Pyrus bretschneideri ), and peach ( Prunus persica ). In total, 35 CCoAOMT genes were identified in the three Rosaceae species: 8 from chinese white pear, 12 from apple, and 15 from peach. By using structure analysis and collinearity analysis, we found 12 conserved motifs and 12 pairs of CCoAOMT genes with collinearity. In the phylogenetic tree, the gene family was mainly divided into two groups. The genes had different expression patterns during the growth and development stage of fruit, a finding that is consistent with the pattern of lignin accumulation. This study will be beneficial for further study of CCoAOMT genes.
    Type of Medium: Online Resource
    ISSN: 0003-1062 , 2327-9788
    URL: Article
    DOI: 10.21273/JASHS04950-20
    Language: Unknown
    Publisher: American Society for Horticultural Science
    Publication Date: 2021
    detail.hit.zdb_id: 2040057-3
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