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The order of sequential exposure of U2OS cells to gamma and alpha radiation influences the formation and decay dynamics of NBS1 foci

Tartas, Adrianna ; Lundholm, Lovisa ; Scherthan, Harry ; [et al.]

Public Library of Science (PLoS) ; 2023

In: PLOS ONE Vol. 18, No. 6 ( 2023-6-12), p. e0286902-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 6 ( 2023-6-12), p. e0286902-

Abstract: DNA double strand breaks (DSBs) are a deleterious form of DNA damage. Densely ionising alpha radiation predominantly induces complex DSBs and sparsely ionising gamma radiation—simple DSBs. We have shown that alphas and gammas, when applied simultaneously, interact in producing a higher DNA damage response (DDR) than predicted by additivity. The mechanisms of the interaction remain obscure. The present study aimed at testing whether the sequence of exposure to alphas and gammas has an impact on the DDR, visualised by live NBS1-GFP (green fluorescent protein) focus dynamics in U2OS cells. Focus formation, decay, intensity and mobility were analysed up to 5 h post exposure. Focus frequencies directly after sequential alpha → gamma and gamma → alpha exposure were similar to gamma alone, but gamma → alpha foci quickly declined below the expected values. Focus intensities and areas following alpha alone and alpha → gamma were larger than after gamma alone and gamma → alpha. Focus movement was most strongly attenuated by alpha → gamma. Overall, sequential alpha → gamma exposure induced the strongest change in characteristics and dynamics of NBS1-GFP foci. Possible explanation is that activation of the DDR is stronger when alpha-induced DNA damage precedes gamma-induced DNA damage.

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0286902

DOI: 10.1371/journal.pone.0286902.g001

DOI: 10.1371/journal.pone.0286902.g002

DOI: 10.1371/journal.pone.0286902.g003

DOI: 10.1371/journal.pone.0286902.g004

DOI: 10.1371/journal.pone.0286902.g005

DOI: 10.1371/journal.pone.0286902.g006

DOI: 10.1371/journal.pone.0286902.t001

DOI: 10.1371/journal.pone.0286902.t002

DOI: 10.1371/journal.pone.0286902.t003

DOI: 10.1371/journal.pone.0286902.s001

DOI: 10.1371/journal.pone.0286902.s002

DOI: 10.1371/journal.pone.0286902.s003

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2023

detail.hit.zdb_id: 2267670-3

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Monte Carlo Modeling of DNA Lesions and Chromosomal Aberrations Induced by Mixed Beams of Alpha Particles and X-Rays

Brzozowska, Beata ; Tartas, Adrianna ; Wojcik, Andrzej

Frontiers Media SA ; 2020

In: Frontiers in Physics Vol. 8 ( 2020-11-12)

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In: Frontiers in Physics, Frontiers Media SA, Vol. 8 ( 2020-11-12)

Type of Medium: Online Resource

ISSN: 2296-424X

URL: Article

DOI: 10.3389/fphy.2020.567864

Language: Unknown

Publisher: Frontiers Media SA

Publication Date: 2020

detail.hit.zdb_id: 2721033-9

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Freeware tool for analysing numbers and sizes of cell colonies

Brzozowska, Beata ; Gałecki, Maciej ; Tartas, Adrianna ; [et al.]

Springer Science and Business Media LLC ; 2019

In: Radiation and Environmental Biophysics Vol. 58, No. 1 ( 2019-3), p. 109-117

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In: Radiation and Environmental Biophysics, Springer Science and Business Media LLC, Vol. 58, No. 1 ( 2019-3), p. 109-117

Type of Medium: Online Resource

ISSN: 0301-634X , 1432-2099

URL: Article

DOI: 10.1007/s00411-018-00772-z

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2019

detail.hit.zdb_id: 1462083-2

SSG: 12

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Exosome secretion and cellular response of DU145 and PC3 after exposure to alpha radiation

Pszczółkowska, Beata ; Olejarz, Wioletta ; Filipek, Mateusz ; [et al.]

Springer Science and Business Media LLC ; 2022

In: Radiation and Environmental Biophysics Vol. 61, No. 4 ( 2022-11), p. 639-650

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In: Radiation and Environmental Biophysics, Springer Science and Business Media LLC, Vol. 61, No. 4 ( 2022-11), p. 639-650

Abstract: Exosomes are spherical membrane nanovesicles secreted from cells, and they play an important role in tumor immune response, metastasis, angiogenesis, and survival. Studies investigating exosomes isolated from cells exposed to photon radiation commonly used in conventional radiotherapy demonstrate the influence of this type of radiation on exosome characteristics and secretion. There is currently no research investigating the effects of densely ionizing particles such as protons and alpha radiation on exosomes. Thus we have evaluated the cellular response of human prostate cancer cells exposed to 0, 2, and 6 Gy of alpha radiation emitted from the Am-241 source. Irradiated PC3 and DU145 cell lines, characterized by differences in radiosensitivity, were studied using apoptosis, LDH, and IL-6 assays. Additionally, the corresponding concentration and size of isolated exosomes were measured using NTA. We found that exposure to ionizing radiation resulted in gross changes in viability and cell damage. There were increased amounts of apoptotic or necrotic cells as a function of radiation dose. We demonstrated that irradiated PC3 cells secrete higher quantities of exosomes compared to DU145 cells. Additionally, we also found no statistical difference in exosome size for control and irradiated cells.

Type of Medium: Online Resource

ISSN: 0301-634X , 1432-2099

URL: Article

DOI: 10.1007/s00411-022-00991-5

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2022

detail.hit.zdb_id: 1462083-2

SSG: 12

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Cell Type-Specific Patterns in the Accumulation of DNA Damage Following Multifractional Radiation Exposure

Akuwudike, Pamela ; Tartas, Adrianna ; López-Riego, Milagrosa ; [et al.]

MDPI AG ; 2022

In: International Journal of Molecular Sciences Vol. 23, No. 21 ( 2022-10-25), p. 12861-

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In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 21 ( 2022-10-25), p. 12861-

Abstract: Predicting the risk of second malignant neoplasms is complicated by uncertainties regarding the shape of the dose–response relationship at high doses. Limited understanding of the competitive relationship between cell killing and the accumulation of DNA lesions at high doses, as well as the effects of other modulatory factors unique to radiation exposure during radiotherapy, such as dose heterogeneity across normal tissue and dose fractionation, contribute to these uncertainties. The aim of this study was to analyze the impact of fractionated irradiations on two cell systems, focusing on the endpoints relevant for cancer induction. To simulate the heterogeneous dose distribution across normal tissue during radiotherapy, exponentially growing VH10 fibroblasts and AHH-1 lymphoblasts were irradiated with 9 and 12 fractions (VH10) and 10 fractions (AHH-1) at 0.25, 0.5, 1, or 2 Gy per fraction. The effects on cell growth, cell survival, radiosensitivity and the accumulation of residual DNA damage lesions were analyzed as functions of dose per fraction and the total absorbed dose. Residual γH2AX foci and other DNA damage markers (micronuclei, nuclear buds, and giant nuclei) were accumulated at high doses in both cell types, but in a cell type-dependent manner. The competitive relationship between cell killing and the accumulation of carcinogenic DNA damage following multifractional radiation exposure is cell type-specific.

Type of Medium: Online Resource

ISSN: 1422-0067

URL: Article

DOI: 10.3390/ijms232112861

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2019364-6

SSG: 12

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ITV, mid-ventilation, gating or couch tracking – A comparison of respiratory motion-management techniques based on 4D dose calculations

Ehrbar, Stefanie ; Jöhl, Alexander ; Tartas, Adrianna ; [et al.]

Elsevier BV ; 2017

In: Radiotherapy and Oncology Vol. 124, No. 1 ( 2017-07), p. 80-88

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In: Radiotherapy and Oncology, Elsevier BV, Vol. 124, No. 1 ( 2017-07), p. 80-88

Type of Medium: Online Resource

ISSN: 0167-8140

URL: Article

DOI: 10.1016/j.radonc.2017.05.016

Language: English

Publisher: Elsevier BV

Publication Date: 2017

detail.hit.zdb_id: 1500707-8

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A matter of space: how the spatial heterogeneity in energy deposition determines the biological outcome of radiation exposure

Baiocco, Giorgio ; Bartzsch, Stefan ; Conte, Valeria ; [et al.]

Springer Science and Business Media LLC ; 2022

In: Radiation and Environmental Biophysics Vol. 61, No. 4 ( 2022-11), p. 545-559

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In: Radiation and Environmental Biophysics, Springer Science and Business Media LLC, Vol. 61, No. 4 ( 2022-11), p. 545-559

Abstract: The outcome of the exposure of living organisms to ionizing radiation is determined by the distribution of the associated energy deposition at different spatial scales. Radiation proceeds through ionizations and excitations of hit molecules with an ~ nm spacing. Approaches such as nanodosimetry/microdosimetry and Monte Carlo track-structure simulations have been successfully adopted to investigate radiation quality effects: they allow to explore correlations between the spatial clustering of such energy depositions at the scales of DNA or chromosome domains and their biological consequences at the cellular level. Physical features alone, however, are not enough to assess the entity and complexity of radiation-induced DNA damage: this latter is the result of an interplay between radiation track structure and the spatial architecture of chromatin, and further depends on the chromatin dynamic response, affecting the activation and efficiency of the repair machinery. The heterogeneity of radiation energy depositions at the single-cell level affects the trade-off between cell inactivation and induction of viable mutations and hence influences radiation-induced carcinogenesis. In radiation therapy, where the goal is cancer cell inactivation, the delivery of a homogenous dose to the tumour has been the traditional approach in clinical practice. However, evidence is accumulating that introducing heterogeneity with spatially fractionated beams (mini- and microbeam therapy) can lead to significant advantages, particularly in sparing normal tissues. Such findings cannot be explained in merely physical terms, and their interpretation requires considering the scales at play in the underlying biological mechanisms, suggesting a systemic response to radiation.

Type of Medium: Online Resource

ISSN: 0301-634X , 1432-2099

URL: Article

DOI: 10.1007/s00411-022-00989-z

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2022

detail.hit.zdb_id: 1462083-2

SSG: 12

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Modeling of dose and linear energy transfer homogeneity in cell nuclei exposed to alpha particles under various setup conditions

Tartas, Adrianna ; Filipek, Mateusz ; Pietrzak, Marcin ; [et al.]

Informa UK Limited ; 2023

In: International Journal of Radiation Biology Vol. 99, No. 8 ( 2023-08-03), p. 1248-1256

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In: International Journal of Radiation Biology, Informa UK Limited, Vol. 99, No. 8 ( 2023-08-03), p. 1248-1256

Type of Medium: Online Resource

ISSN: 0955-3002 , 1362-3095

URL: Article

DOI: 10.1080/09553002.2023.2161659

RVK:

WA 15000

Language: English

Publisher: Informa UK Limited

Publication Date: 2023

detail.hit.zdb_id: 1498203-1

SSG: 12

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Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers

Gałecki, Maciej ; Tartas, Adrianna ; Szymanek, Agata ; [et al.]

Informa UK Limited ; 2019

In: International Journal of Radiation Biology Vol. 95, No. 9 ( 2019-09-02), p. 1251-1258

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In: International Journal of Radiation Biology, Informa UK Limited, Vol. 95, No. 9 ( 2019-09-02), p. 1251-1258

Type of Medium: Online Resource

ISSN: 0955-3002 , 1362-3095

URL: Article

DOI: 10.1080/09553002.2019.1625462

RVK:

WA 15000

Language: English

Publisher: Informa UK Limited

Publication Date: 2019

detail.hit.zdb_id: 1498203-1

SSG: 12

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The Risk Function of Breast and Ovarian Cancers in the Avrami–Dobrzyński Cellular Phase-Transition Model

Zawadzka, Anna ; Brzozowska, Beata ; Matyjanka, Anna ; [et al.]

MDPI AG ; 2024

In: International Journal of Molecular Sciences Vol. 25, No. 2 ( 2024-01-22), p. 1352-

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In: International Journal of Molecular Sciences, MDPI AG, Vol. 25, No. 2 ( 2024-01-22), p. 1352-

Abstract: Specifying the role of genetic mutations in cancer development is crucial for effective screening or targeted treatments for people with hereditary cancer predispositions. Our goal here is to find the relationship between a number of cancerogenic mutations and the probability of cancer induction over the lifetime of cancer patients. We believe that the Avrami–Dobrzyński biophysical model can be used to describe this mechanism. Therefore, clinical data from breast and ovarian cancer patients were used to validate this model of cancer induction, which is based on a purely physical concept of the phase-transition process with an analogy to the neoplastic transformation. The obtained values of model parameters established using clinical data confirm the hypothesis that the carcinogenic process strongly follows fractal dynamics. We found that the model’s theoretical prediction and population clinical data slightly differed for patients with the age below 30 years old, and that might point to the existence of an ancillary protection mechanism against cancer development. Additionally, we reveal that the existing clinical data predict breast or ovarian cancers onset two years earlier for patients with BRCA1/2 mutations.

Type of Medium: Online Resource

ISSN: 1422-0067

URL: Article

DOI: 10.3390/ijms25021352

Language: English

Publisher: MDPI AG

Publication Date: 2024

detail.hit.zdb_id: 2019364-6

SSG: 12

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