In:
Annual Review of Plant Biology, Annual Reviews, Vol. 68, No. 1 ( 2017-04-28), p. 197-224
Abstract:
The plant endomembrane system is an extensively connected functional unit for exchanging material between compartments. Secretory and endocytic pathways allow dynamic trafficking of proteins, lipids, and other molecules, regulating a myriad of biological processes. Chemical genetics—the use of compounds to perturb biological processes in a fast, tunable, and transient manner—provides elegant tools for investigating this system. Here, we review how chemical genetics has helped to elucidate different aspects of membrane trafficking. We discuss different strategies for uncovering the modes of action of such compounds and their use in unraveling membrane trafficking regulators. We also discuss how the bioactive chemicals that are currently used as probes to interrogate endomembrane trafficking were discovered and analyze the results regarding membrane trafficking and pathway crosstalk. The integration of different expertises and the rational implementation of chemical genetic strategies will improve the identification of molecular mechanisms that drive intracellular trafficking and our understanding of how trafficking interfaces with plant physiology and development.
Type of Medium:
Online Resource
ISSN:
1543-5008
,
1545-2123
DOI:
10.1146/arplant.2017.68.issue-1
DOI:
10.1146/annurev-arplant-042916-041107
Language:
English
Publisher:
Annual Reviews
Publication Date:
2017
detail.hit.zdb_id:
2098209-4
SSG:
12
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