In:
Journal of Clinical Oncology, American Society of Clinical Oncology (ASCO), Vol. 35, No. 15_suppl ( 2017-05-20), p. e20011-e20011
Abstract:
e20011 Background: Small cell lung cancer (SCLC), which accounts for 15-20% of lung cancer, is a highly aggressive neoplasm. Different from non-small cell lung cancer, SCLC is characterized by a high proliferation rate and early metastasis. Only about 25% of patients with limited stage disease will be cured after first-line standard treatment and majority of the patients will relapse after initial sensitivity to the chemotherapy and radiotherapy. The highly complex genetic landscape of SCLC may be the reason for its resistance to conventional therapy but the details are still unclear. Methods: Nine patients with SCLC received platinum-based chemotherapy and/or radiotherapy were included in this study. According to the prognosis, patients were classified into two groups: poor prognosis group (PP group, PFS 〈 6 months, n = 5) and better prognosis group (BP group, PFS 〉 24 months, n = 4). Tumor DNA was isolated from pre-treatment paraffin-embedded tumor tissue and subjected to enrichment for a 1.15M size panel cover exon regions from 1,086 genes. Followed by next generation sequencing on an Illumina X10 platform, the captured sequencing data was further processed using bioinformatics analysis to identify somatic mutations, including single nucleotide variants (SNV), short insertions/deletions (indels) and copy number variation (CNV). Changes in tumor DNA were compared with clinical efficiency. Results: We show that the tumor mutation burden in PP group is higher than the BP group (p = 0.06). Using the mutation burden of 12 genes (PTEN, TP53, JAK2, BCL2, CCNE1, EGFR, ROS1, NTRK3, MYC, FGFR1, RANBP2, and CREBBP) as a factor, the PP group can be distinguished from BP group. In addition, TP53 loss is more frequently appeared in the PP group. Conclusions: Our results suggest that high mutation burden status of 12 genes (PTEN, TP53, JAK2, BCL2, CCNE1, EGFR, ROS1, NTRK3, MYC, FGFR1, RANBP2, and CREBBP) might correlate with poor prognosis of small cell lung cancer.
Type of Medium:
Online Resource
ISSN:
0732-183X
,
1527-7755
DOI:
10.1200/JCO.2017.35.15_suppl.e20011
Language:
English
Publisher:
American Society of Clinical Oncology (ASCO)
Publication Date:
2017
detail.hit.zdb_id:
2005181-5
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