In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2022-5-26), p. e0266620-
Abstract:
Small proline-rich protein 1A (SPRR1A) is recognized as a squamous differentiation marker but is also upregulated in some non-squamous cancers. However, its expression in pancreatic ductal adenocarcinoma (PDAC) has not been investigated. This study elucidated the expression of SPRR1A in PDAC and its effect on the prognosis and malignant behavior of PDAC. Methods We examined the SPRR1A expression by immunohistochemistry in 86 surgical PDAC cases and revealed the relationship between its expression and the prognosis of the PDAC patients. Furthermore, we overexpressed SPRR1A in pancreatic cancer cell lines (PK-1 and Panc-1) and assessed the phenotype and gene expression changes in vitro . Results Among the 84 cases, excluding 2 with squamous differentiation, 31 (36.9%) had a high SPRR1A expression. The overall survival (median 22.1 months vs. 33.6 months, p = 0.0357) and recurrence-free survival (median 10.7 months vs. 15.5 months, p = 0.0298) were significantly lower in the high-SPRR1A-expression group than in the low-SPRR1A-expression group. A multivariate analysis indicated that a high SPRR1A expression (HR 1.706, 95% CI 1.018 to 2.862, p = 0.0427) and residual tumor status (HR 2.687, 95% CI 1.487 to 4.855, p = 0.00106) were independent prognostic factors. The analysis of TCGA transcriptome data demonstrated that the high-SPRR1A-expression group had a significantly worse prognosis than the low-SPRR1A-expression group, which supported our data. SPRR1A overexpression in PK-1 and Panc-1 did not result in remarkable changes to in vitro phenotypes, such as the cell proliferation, chemo-resistance, EMT, migration or global gene expression. Conclusion Increased expression of SPRR1A is associated with a poor prognosis in PDAC and may serve as a novel prognostic marker. However, our in vitro study suggests that the SPRR1A expression may be a consequence, not a cause, of the aggressive behavior of PDAC.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0266620
DOI:
10.1371/journal.pone.0266620.g001
DOI:
10.1371/journal.pone.0266620.g002
DOI:
10.1371/journal.pone.0266620.g003
DOI:
10.1371/journal.pone.0266620.g004
DOI:
10.1371/journal.pone.0266620.t001
DOI:
10.1371/journal.pone.0266620.t002
DOI:
10.1371/journal.pone.0266620.s001
DOI:
10.1371/journal.pone.0266620.s002
DOI:
10.1371/journal.pone.0266620.s003
DOI:
10.1371/journal.pone.0266620.s004
DOI:
10.1371/journal.pone.0266620.s005
DOI:
10.1371/journal.pone.0266620.s006
DOI:
10.1371/journal.pone.0266620.s007
DOI:
10.1371/journal.pone.0266620.s008
DOI:
10.1371/journal.pone.0266620.s009
DOI:
10.1371/journal.pone.0266620.s010
DOI:
10.1371/journal.pone.0266620.s011
DOI:
10.1371/journal.pone.0266620.s012
DOI:
10.1371/journal.pone.0266620.s013
DOI:
10.1371/journal.pone.0266620.r001
DOI:
10.1371/journal.pone.0266620.r002
DOI:
10.1371/journal.pone.0266620.r003
DOI:
10.1371/journal.pone.0266620.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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