In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 80, No. 18 ( 1983-09), p. 5530-5534
Abstract:
Certain Escherichia coli rho mutations, exemplified by rho026 , block the growth of phage λ by interfering with phage gene expression. The phage gene N , whose product suppresses transcription termination, appears to be expressed normally in the mutants, and the functional stability of the N protein is not affected. Our data suggest that these rho mutations allow transcription to terminate despite the presence of N. Other E. coli mutants displaying a similar phenotype (Nus - ) fail to propagate wild-type λ but permit the growth of the λ variant λ nin5 , which has undergone a deletion of the λ terminator t R2 . The phenotype of the rho026 mutant differs: the growth of λ is only marginally improved by the nin5 deletion. Interestingly, N activity at rho-independent terminators is not inhibited by the mutations, whereas its ability to suppress rho-dependent terminators is markedly reduced. The relevance of this specificity in terms of models of N action is discussed.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.80.18.5530
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1983
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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