In:
cclm, Walter de Gruyter GmbH, Vol. 38, No. 8 ( 2000-08-21), p. 743-745
Abstract:
The aim of our work was to develop a method to determine the the H + /K + -ATPase activity of human gastric biopsy samples. Our method is based on the phosphatase activity and the K + -induceable property of the enzyme. K + -induceable pNPPase activity was determined from homogenated corpus and antrum biopsy samples. H + /K + -ase activity was calculated as the difference between the corpus and antrum K + -induceable pNPPase activities. Quality control measurements were done during 20 successive days from pooled homogenates. The total, between-day and between-run, within-day and within-run coefficients of variations were between 10 and 16%. The healthy mean and reference range of K + -induceable pNPPase activity in the corpus was 95.8 (95% CI: 83.4–108.2 mU/mg protein); in the antrum it was 28.3 (21.6–35.0) mU/mg protein. The calculated H + /K + -ATPase activity was 67.2 (56.9–77.5) mU/mg protein. The measured activities were independent of the age and gender. Summarizing our results we have concluded, that our novel method might be a potential tool to gather data about the functional acid producing capability of human gastric mucosa.
Type of Medium:
Online Resource
ISSN:
1434-6621
DOI:
10.1515/CCLM.2000.105
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
2000
detail.hit.zdb_id:
1492732-9
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